| 蛇床子素与金雀异黄酮对大鼠峰值骨量影响的比较研究 |
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投稿时间：2013-05-29
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| 作者 | Author | 单位 | Address | E-Mail |
| 成魁 |
CHENG Kui |
兰州军区兰州总医院骨科研究所, 甘肃 兰州 730050 |
Institute of Orthopaedics, Lanzhou General Hospital of Lanzhou Military Area, Lanzhou 730050, Gansu, China |
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| 葛宝丰 |
GE Bao-feng |
兰州军区兰州总医院骨科研究所, 甘肃 兰州 730050 |
Institute of Orthopaedics, Lanzhou General Hospital of Lanzhou Military Area, Lanzhou 730050, Gansu, China |
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| 甄平 |
ZHEN Ping |
兰州军区兰州总医院骨科研究所, 甘肃 兰州 730050 |
Institute of Orthopaedics, Lanzhou General Hospital of Lanzhou Military Area, Lanzhou 730050, Gansu, China |
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| 陈克明 |
CHEN Ke-ming |
兰州军区兰州总医院骨科研究所, 甘肃 兰州 730050 |
Institute of Orthopaedics, Lanzhou General Hospital of Lanzhou Military Area, Lanzhou 730050, Gansu, China |
chkeming@aliyun.com |
| 马小妮 |
MA Xiao-ni |
兰州军区兰州总医院骨科研究所, 甘肃 兰州 730050 |
Institute of Orthopaedics, Lanzhou General Hospital of Lanzhou Military Area, Lanzhou 730050, Gansu, China |
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| 周建 |
ZHOU Jian |
兰州军区兰州总医院骨科研究所, 甘肃 兰州 730050 |
Institute of Orthopaedics, Lanzhou General Hospital of Lanzhou Military Area, Lanzhou 730050, Gansu, China |
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| 宋鹏 |
SONG Peng |
兰州军区兰州总医院骨科研究所, 甘肃 兰州 730050 |
Institute of Orthopaedics, Lanzhou General Hospital of Lanzhou Military Area, Lanzhou 730050, Gansu, China |
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| 马慧萍 |
MA Hui-ping |
兰州军区兰州总医院药材科, 甘肃 兰州 730050 |
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| 期刊信息：《中国骨伤》2014年，第27卷，第7期，第587-591页 |
| DOI：10.3969/j.issn.1003-0034.2014.07.013 |
| 基金项目:国家自然科学基金（编号：81270963）；甘肃省科技重大专项（092NKDA025） |
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中文摘要:
目的：研究蛇床子素（Osthole，OST）与金雀异黄酮（Genistein，GEN）对大鼠峰值骨密度与骨质量的影响。
方法：采用随机分组法将36只1月龄SD雌性大鼠（125±3） g分为3组：对照组（CON，等体积蒸馏水，n=12），蛇床子素组（OST，9 mg·kg-1d-1 i.g.，n=12），金雀异黄酮组（GEN，10 mg·kg-1d-1 i.g.；n=12）.每周监测体重，每月用双能X射线骨密度仪（DEXA）检测全身骨密度。3个月后处死所有动物，采用酶联免疫法测定血清骨钙素（Osteocalcin，OC）和抗酒石酸性磷酸酶5b（Tartaric acid phosphatase 5b，TRACP 5b）含量，用DEXA测定股骨骨密度，用μCT分析股骨组织微结构，树脂包埋不脱钙骨组织切片技术做胫骨骨形态分析，用万能材料试验机测定股骨生物力学，称量心、肝、胃、肾、肾上腺和子宫湿重，计算器官指数，并做常规病理学检测。
结果：大鼠的体重、器官指数差异无统计学意义（P>0.05）；病理学观察未见异常发生；第1、2个月全身骨密度无明显差异（P>0.05），第3个月后OST组全身骨密度显着高于CON组、GEN组，上述3组中股骨骨密度与全身骨密度的变化呈相同趋势（P<0.05）.与对照组相比，OST组、GEN组血清OC水平升高，而与CON组、GEN组相比，OST组的TRACP 5b含量下降（P<0.05）；OST组的骨体积百分率、骨小梁厚度和骨小梁数量均高于CON组，但骨小梁分离度和模型系数均显着低于CON组（P<0.05），OST组与GEN组的上述指标虽未出现统计学差异（P>0.05），但平均值OST组高于GEN组；OST组骨小梁厚度和骨小梁数量均高于CON组、GEN组，但骨小梁分离度低于CON组、GEN组；OST组股骨最大载荷、弹性模量和屈服强度均明显高于CON组（P<0.05），OST组与GEN组虽未出现统计学差异（P>0.05），但平均值OST组高于GEN组。
结论：口服蛇床子素能有效提高大鼠峰值骨量，从而为防治骨质疏松的发生提供新思路。 |
| 【关键词】蛇床子素 金雀异黄酮 骨密度 骨和骨组织 大鼠 |
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| Comparative study on effect of osthole and genistein on peak bone mass in rats |
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ABSTRACT
Objective: To compare the ability of osthole (OST) and genistein (GEN) in enhancing bone peak bone mass of rats to prevent osteoporosis.
Methods: Thirty-six female one-month-old SD rats of(125±3) g body weight were randomly divided into three groups,12 rats in each group,one group was orally administered osthole at 9 mg·kg-1d-1,one group was given genistein at 10 mg·kg-1d-1 and another was given equal quantity of distilled water as the control. The body weight was monitored weekly and the bone mineral density (BMD) of total body was measured every month. All rats were sacrificed after three months,the femoral bone mineral density,the serum levels of osteocalcin (OC) and anti-tartaric acid phosphatase 5b (TRACP 5b) were measured by Elisa. The bone microarchitectures were analyzed with micro-CT and the bone biomechanics properties were tested with universal material machine.
Results: No significant differences were observed between O-treated or GEN group and the control for the food-intake and body weight during three months. However,the rats treated with OST had significant higher BMD for both total body and femur than the control and GEN group. The O-treated rats also had higher level of serum OC and lower level of TRACP 5b. Besides,they owned bigger bone volume/tissue volume,trabecular thickness,trabecular number but smaller trabecular spacing. In the three point bending tests of femurs,they were found to have larger maximum load,the young's modulus and structural model index(SMI).
Conclusion: Orally administered osthole could efficiently increase the peak bone mass of rats,which provide new ideas for preventing osteoporosis. |
| KEY WORDS Osthole Genistein Bone density Bone and bones Rats |
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| 中文格式: | 成魁,葛宝丰,甄平,陈克明,马小妮,周建,宋鹏,马慧萍.蛇床子素与金雀异黄酮对大鼠峰值骨量影响的比较研究[J].中国骨伤,2014,27(7):587~591 |
| 英文格式: | CHENG Kui,GE Bao-feng,ZHEN Ping,CHEN Ke-ming,MA Xiao-ni,ZHOU Jian,SONG Peng,MA Hui-ping.Comparative study on effect of osthole and genistein on peak bone mass in rats[J].zhongguo gu shang / China J Orthop Trauma ,2014,27(7):587~591 |
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