| 土鳖虫对激素诱导骨髓间充质干细胞成骨分化的影响 |
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投稿时间：2010-03-25
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| 期刊信息：《中国骨伤》2010年，第23卷，第12期，第921-924页 |
| DOI：10.3969/j.issn.1003-0034.2010.12.012 |
| 基金项目:福建省科技计划项目(编号：2007F5060) |
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中文摘要:
目的：探讨激素性股骨头缺血坏死的发病机制及土鳖虫防治该病的作用机制。
方法：体外培养骨髓间充质干细胞，传3代BMSCs随机分为空白组，模型组及中药低、中、高剂量组。模型组应用大剂量地塞米松诱导体外培养的骨髓间充质干细胞成脂分化，抑制其成骨分化。中药低、中、高剂量组在诱导成脂的同时给予土鳖虫含药血清干预。检测干预6 d后各组细胞内成骨标志物骨钙素、碱性磷酸酶及Ⅰ型胶原 mRNA的表达。
结果：土鳖虫含药血清可逆转激素诱导下的BMSCs内碱性磷酸酶含量，骨钙素及Ⅰ型胶原 mRNA表达均降低。
结论：土鳖虫防治激素性股骨头缺血坏死的机制不仅是改善股骨头的微循环，同时还与其抑制激素诱导下的BMSCs成骨分化减少有关。 |
| 【关键词】股骨头坏死 活血化瘀剂 骨髓 间质干细胞 |
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| Influence of Ground Beetle(土鳖虫) on steroid-induced BMSCs osteogenic differentiation |
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ABSTRACT
Objective: To investigate the pathogenesis of steroid-induced avascular necrosis of femoral head and the control mechanisms of Ground Beetle(土鳖虫).
Methods: The bone marrow-derived mesenchymal stem cells were cultured in vitro. The BMSCS in the 3rd generation were randomly divided into blank group,model group and Chinese medicine low,medium and high dose group. BMSCS of model group using high-dose steroid-induced in vitro were adipogenic differentiation to inhibite osteogenesis. Chinese medicine low,medium and high-dose groups at the same time were given the Ground Beetle(土鳖虫) intervention serum containing insects. The expression of osteocalcin,Alkaline phosphatase and Type I Collagen mRNA were detected and interfered 6 days later.
Results: Serum containing Ground Beetle(土鳖虫) could reverse content of steroid-induced alkaline phosphatase of BMSCS,the expression of ostercalcin and Type I Collagen mRNA decreased.
Conclusion: The control mechanism of Ground Beetle(土鳖虫) on steroid-induced avascular necrosis is not only to improve the microcirculation,but also to inhibite steroid-induced osteogenic differentiation of BMSCs reduced. |
| KEY WORDS Femur head necrosis Blood act stasis remov agents Bone marrow Mesenchymal stem cells |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 李树强,于涛,齐振熙.土鳖虫对激素诱导骨髓间充质干细胞成骨分化的影响[J].中国骨伤,2010,23(12):921~924 |
| 英文格式: | LI Shu-qiang,YU Tao,QI Zhen-xi.Influence of Ground Beetle(土鳖虫) on steroid-induced BMSCs osteogenic differentiation[J].zhongguo gu shang / China J Orthop Trauma ,2010,23(12):921~924 |
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