| 骨康对去卵巢大鼠骨源性碱性磷酸酶和胰岛素样生长因子的影响 |
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投稿时间：2008-09-11
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| 期刊信息：《中国骨伤》2009年，第22卷，第2期，第119-121页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目: |
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中文摘要:
目的：探讨骨康对去卵巢大鼠血清中骨碱性磷酸酶(BALP)和胰岛素样生长因子-1(IGF-1)的影响，及其治疗骨质疏松症的作用机制。
方法：6月龄雌性SD大鼠68只按简单随机方法分为空白组和手术组，分别为22只和46只。空白组行假手术，手术组行卵巢切除术。术后3个月后从两组随机各选出10只大鼠行骨密度检查，以确定造模成功。在确定造模成功后，将手术组中剩下的36只大鼠再随机分为3组，每组12 只，分别为手术模型组、雌激素组、中药组。空白组中剩下的12只大鼠为空白对照组。对各组进行灌胃给药治疗，灌胃给药容积为10 ml/kg,空白对照组、手术模型组灌服蒸馏水；中药组灌服相应的药物，雌激素组给予尼尔雌醇灌胃。治疗3个月后采取动物血清进行实验指标检测，测定动物血清中E2、IGF-1、BALP含量。
结果：雌激素组、中药组血清E2、BALP水平高于手术模型组，其差异有统计学意义(P<0.05),而雌激素组与中药组血清E2水平相比较差异无统计学意义(P>0.05).中药组血清IGF-1水平高于手术模型组及空白对照组，差异有统计学意义(P<0.05).
结论：中药骨康能提高去卵巢大鼠血清中雌二醇、胰岛素样生长因子、骨源性碱性磷酸酶水平，间接促进成骨细胞增殖，抑制破骨细胞骨吸收，促进成骨细胞的骨形成功能。 |
| 【关键词】骨康 骨碱性磷酸酶 胰岛素样生长因子1 骨质疏松 去卵巢大鼠 |
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| Effects of Gukang(骨康) on bone-source alkaline phosphatase(BALP) and insulin-like growth factor-1(IGF-1) in serum of spaying rats |
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ABSTRACT
Objective: To investigate the effects of Gukang on bone-source alkaline phosphatase(BALP) and insulin-like growth factor 1(IGF-1) in serum of spaying rats and the mechanism of curative effect of Gukang on osteoporosis.
Methods: Sixty-eight 6-month-old SD rats were chosen and randomly divided into blank control group(22 rats with sham operation) and operation group(46 rats with spaying operation). Three months after operation,10 rats were randomly chosen from each group and tested with bone mineral density in order to determine models of osteoporosis made. After modeling,operation group was divided into 3 sub-groups:operation model group,estrogen group and Gukang group,12 rars in each group. Twelve rats remained in blank control group. Every group were treated through intragastric administration therapy(volum 10 ml/kg). Blank control group and operation model group were irrigated with distilled water,estrogen group with estrogen and Gukang group with Gukang. Three months after treatment,serum of all groups were collected and tested for E2,BALP and IGF-1 with ELISA.
Results: The concentration of serum E2,BALP in estrogen group and Gukang group were higher than operation model group,there were significant difference(P<0.05),but no significant difference in serum E2 between estrogen group and Gukang group (P>0.05). The concentration of serum IGF-1 in Gukang group was higher than operation model group and blank control group,there were significant difference(P<0.05).
Conclusion: Gukang can increase the level of E2,BALP and IGF-1 in serum of spaying rats. Thus,it can indirectly promote reproduction of osteoblasts,inhibit activity of osteoclasts and promote bone formation. |
| KEY WORDS Gukang(骨康) Bone-source alkaline phosphatases(BALP) Insulin-like growth factor 1(IGF-1) Osteoporosis Spaying rats |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 陈一凡,黄宏兴,李颖.骨康对去卵巢大鼠骨源性碱性磷酸酶和胰岛素样生长因子的影响[J].中国骨伤,2009,22(2):119~121 |
| 英文格式: | CHEN Yi-fan,HUANG Hong-xing,LI Ying.Effects of Gukang(骨康) on bone-source alkaline phosphatase(BALP) and insulin-like growth factor-1(IGF-1) in serum of spaying rats[J].zhongguo gu shang / China J Orthop Trauma ,2009,22(2):119~121 |
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