| 退行性腰椎滑脱黄韧带病理组织学和免疫组化的观察 |
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投稿时间：2004-03-01
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| 作者 | Author | 单位 | Address | E-Mail |
| 毛兆光 |
MAO Zhao-guang |
浙江大学医学院附属邵逸夫医院骨科,浙江杭州310016 |
Department of Orthopaedics,Sir Run Run Shaw Hospital,Medical College of Zhejiang University,Zhejiang Hangzhou,310016,China |
mzg0906 @163. com |
| 范顺武 |
FAN Shun-wu |
浙江大学医学院附属邵逸夫医院骨科,浙江杭州310016 |
Department of Orthopaedics,Sir Run Run Shaw Hospital,Medical College of Zhejiang University,Zhejiang Hangzhou,310016,China |
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| 赵凤东 |
ZHAO Feng-dong |
浙江大学医学院附属邵逸夫医院骨科,浙江杭州310016 |
Department of Orthopaedics,Sir Run Run Shaw Hospital,Medical College of Zhejiang University,Zhejiang Hangzhou,310016,China |
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| 朱有法 |
ZHU You-fa |
浙江大学医学院病理学教研室 |
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| 期刊信息：《中国骨伤》2005年，第18卷，第1期，第32-34页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目: |
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中文摘要:
目的:探讨退行性腰椎滑脱症患者黄韧带椎板间部组织学特征和新生成胶原的主要种类。
方法:对手术切除的退行性腰椎滑脱椎板间部黄韧带进行常规HE染色和Masson三色改良法胶原染色,同时用免疫组化的方法检测退变黄韧带中Ⅱ型胶原的表达情况,普通光镜观察。
结果:退行性腰椎滑脱组黄韧带弹力纤维减少,胶原成分显著增加,结构紊乱;免疫组化示基质染色明显深于对照组,其浅黄色至棕褐色阳性表达产物也见于成纤维细胞、成软骨细胞和软骨细胞的胞浆中,Ⅱ型胶原表达阳性率和表达强度与正常组之间差异均具有显著性(P<001或P<005)。
结论:退行性腰椎滑脱黄韧带退变增生肥厚,并有软骨化和骨化倾向,新生的胶原以Ⅱ型胶原为主。 |
| 【关键词】腰椎滑脱 黄韧带 病理学 免疫组化 Ⅱ型胶原 |
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| Pathological and immunohistochemical observation on the ligamentum flavum of degenerative lumbar spondylolisthesis |
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ABSTRACT
Objective:To study the pathological features and the type of proliferative collagen of the interlaminar portion of the ligamentum flavum in degenerative lumbar spondylolisthesis(DLS).
Methods:Pathological observation with HE staining and Masson trichrome staining and immunostaining for collagen type Ⅱ in DLS were done under optical microscope using surgical specimens of the interlaminar portion of ligamentum flavum from patients.
Results::The degenerative ligamentum flavum showed decrease of the elastic fibers and significant increase of collagen fibers,with distorted fibrous structure.The immunostaining of collagen type Ⅱ was more marked in the extracellular matrix of ligamentum flavum in DLS group than that of the control group.Collagen type Ⅱ was also presented in the cytoplasm of fibroblasts,chondroblasts and chondrocytes.The positive expression rate and expression intensity of collagen type Ⅱ in the cell of ligamentum flavum were statistical significane between the DLS group and the control group(P<0.01 or P<0.05).
Conclusion:The kind of proliferative collagen in the ligamentum flavum of degenerative lumbar spondylolisthesis is mainly collagen type Ⅱ.There is tendency of chondrogenesis and ossification of the ligamentum flavum in degenerative lumbar spondylolisthesis. |
| KEY WORDS Lumbar spondylolisthesis Ligamentum flavum Pathology Immunohistochemistry Collagen type Ⅱ |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 毛兆光,范顺武,赵凤东,朱有法.退行性腰椎滑脱黄韧带病理组织学和免疫组化的观察[J].中国骨伤,2005,18(1):32~34 |
| 英文格式: | MAO Zhao-guang,FAN Shun-wu,ZHAO Feng-dong,ZHU You-fa.Pathological and immunohistochemical observation on the ligamentum flavum of degenerative lumbar spondylolisthesis[J].zhongguo gu shang / China J Orthop Trauma ,2005,18(1):32~34 |
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