| 大鼠慢性脊髓受压及减压后骨骼肌细胞的凋亡 |
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投稿时间：2003-11-19
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| 作者 | Author | 单位 | Address | E-Mail |
| 刘京升 |
LIU Jing-sheng |
兰州医学院第二附属医院骨科,甘肃兰州730000 |
Department of Orthopaedics,The Second Affiliated Hospital,Medical School of Lanzhou |
jshliu@eyou.com |
| 孙正义 |
SUN Zheng-yi |
兰州医学院第二附属医院骨科,甘肃兰州730000 |
Department of Orthopaedics,The Second Affiliated Hospital,Medical School of Lanzhou |
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| 王栓科 |
WANG Shuan-ke |
兰州医学院第二附属医院骨科,甘肃兰州730000 |
Department of Orthopaedics,The Second Affiliated Hospital,Medical School of Lanzhou |
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| 汪玉良 |
WANG Yu-liang |
兰州医学院第二附属医院骨科,甘肃兰州730000 |
Department of Orthopaedics,The Second Affiliated Hospital,Medical School of Lanzhou |
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| 期刊信息：《中国骨伤》2004年，第17卷，第8期，第474-476页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目: |
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中文摘要:
目的:研究大鼠慢性脊髓损伤受压及减压后骨骼肌细胞的凋亡。
方法:45只SD大鼠分成3组:①受压组15只,作成慢性脊髓受压损伤模型;②减压组25只,在压迫模型作成10d后取出压迫装置;③正常对照组5只,不做任何处理。应用HE染色、TUNEL和原位杂交技术研究慢性脊髓受压及减压后肌细胞的萎缩和凋亡。
结果:正常组鲜见凋亡细胞,受压组出现骨骼肌细胞凋亡,bcl2mRNA表达减弱,BaxmRNA表达增强;减压后肌细胞凋亡数量明显减少,bcl2mRNA表达增强而BaxmRNA表达减少。
结论:慢性脊髓受压肌细胞凋亡是失神经骨骼肌萎缩的机制之一。 |
| 【关键词】脊髓损伤 骨骼肌 细胞凋亡 |
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| Apoptosis of skeletal muscle cells following chronic compression and decompression of rats spinal cord injury |
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ABSTRACT
Objective:To study the apoptosis of skeletal musculocytes after chronic compression and decompression of the rats spinal cord injury.
Methods:Forty-five SD rats were divided into three groups:Group A(chronic degree compressive injury of spinal cord) of fifteen samples and group B (decompression) of twenty-five samples and group C (normal control) of five samples.HE and TUNEL staining and hybridization in situ were used to investigate the apoptosis and atrophy of muscles.
Results:Apoptosis nucleus of skeletal muscle were rarely seen in group C,but increased in group A and decreased in group B.The expression of bcl-2mRNA was decreased in group A and increased in group B,on the contrary,the expression of BaxmRNA increased in group A and decreased in group B.
Conclusion:Apoptosis of muscleus is an important morphological change after chronic compressive injury of spine cord.Aopotosis is one of the reason for atrophy of skeletal muscle.The expression of bcl-2 and Bax mRNA related with apoptosis of muscle following chronic compressed injury of spinal cord. |
| KEY WORDS Spinal cord injuries Skeletal muscle Apoptosis |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 刘京升,孙正义,王栓科,汪玉良.大鼠慢性脊髓受压及减压后骨骼肌细胞的凋亡[J].中国骨伤,2004,17(8):474~476 |
| 英文格式: | LIU Jing-sheng,SUN Zheng-yi,WANG Shuan-ke,WANG Yu-liang.Apoptosis of skeletal muscle cells following chronic compression and decompression of rats spinal cord injury[J].zhongguo gu shang / China J Orthop Trauma ,2004,17(8):474~476 |
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