| 降钙素基因相关肽对大鼠成骨细胞增殖和分化的影响 |
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投稿时间：2001-03-20
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| 期刊信息：《中国骨伤》2002年，第15卷，第1期，第20-22页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目: |
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中文摘要:
目的:探讨外源性降钙素基因相关肽(CGRP)对大鼠颅盖骨来源成骨细胞的影响,以进一步了解CGRP促进成骨的作用机制。
方法:利用二次酶消化法培养的成骨细胞,加入含不同浓度CGRP的条件培养液,24小时后通过MTT比色,3H-TdR掺入以及细胞内碱性磷酸酶(ALP)含量的测定来观察CGRP对成骨细胞增殖和分化的影响。
结果:CGRP可以明显促进成骨细胞增殖,而且作用呈剂量依赖性。CGRP对细胞内ALP含量无明显影响。
结论:CGRP能够直接作用于成骨细胞并通过促进其增殖而有利于骨形成。 |
| 【关键词】降钙素基因相关肽 成骨细胞 骨再生 |
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| The effects of calcitonin gene-relate peptide on proliferation and differentiation of rat osteoblasts |
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ABSTRACT
Objective:To explicate the effects of exogenous calcitonin gene-related peptide(CGRP)on rat osteoblasts in vitro,and to investigate the mechanism by which CGRP promotes bone formation.
Methods:The osteoblasts were isolated from newborn rat calvaria by sequential enzyme digestion,and the secondary cells were treated with different concentrations of CGRP respectively.After 24 hours of incubation,the effects of CGRP on proliferation and differentiation of osteoblasts were observed with MTT assay, 3H-TdR incorporation and the determination of intracellular ALPase activity.
Results:CGRP could stimulate the proliferation of osteoblasts in dose-dependent manner,but was unable to affect the intracellular ALPase activity of osteoblasts.
Conclusion:CGRP can act directly on osteoblasts and increase bone formation by stimulating the proliferation of osteoblasts,but it does not promote the differentiation of osteoblasts. |
| KEY WORDS Calcitonin gene-related peptide Osteoblast Bone regeneration |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 廉凯,杜靖远,张银刚.降钙素基因相关肽对大鼠成骨细胞增殖和分化的影响[J].中国骨伤,2002,15(1):20~22 |
| 英文格式: | LIAN Kai,DU Jing-yuan,ZHANG Yin-gang..The effects of calcitonin gene-relate peptide on proliferation and differentiation of rat osteoblasts[J].zhongguo gu shang / China J Orthop Trauma ,2002,15(1):20~22 |
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