| IGF-Ⅰ对培养兔关节软骨细胞作用的实验研究 |
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投稿时间：1999-12-24 修订日期：2000-03-03
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| 期刊信息：《中国骨伤》2001年，第14卷，第6期，第341-342页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目: |
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中文摘要:
目的：了解胰岛素样生长因子Ⅰ(IGFⅠ)对培养关节软骨细胞增殖及代谢的影响。
方法：体外单层培养兔关节软骨细胞,实验组以10,100,200ng/mlIGFⅠ作用细胞2、4、6天,对照组不加IGFⅠ作用培养细胞。检测细胞DNA及基质中糖醛酸含量。用流式细胞仪分析在100ng/mlIGFⅠ作用下细胞周期亚时相。
结果：IGFⅠ在10～100ng/ml浓度范围内,对培养软骨细胞增殖和代谢有促进作用,以100ng/ml浓度作用效果最佳。细胞周期分析,实验组DNA合成前期(G1期)较对照组缩短。
结论：IGFⅠ促进软骨细胞的增殖与代谢,且通过缩短细胞G1期而缩短细胞周期。 |
| 【关键词】胰岛素样生长因子Ⅰ 关节疾病 软骨 |
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| Effects of IGF-Ⅰ on proliferation and metabolism of cultured rabbit artiular chondrocytes |
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ABSTRACT
Objective:To investigate the effect of insulin like growth factor Ⅰ(IGF Ⅰ) on proliferation and metabolism of cultured articular chondrocytes.
Methods:The monolayer chondrocytes obtained from rabbit were cultured.The experimental group was treated with IGF Ⅰ at doses ranging from 10,100,200ng/ml at 2,4,and 6 days while the control group was cultured without IGF Ⅰ.The cell DNA content and matrical glucuronic acid were maesured.The cell cycle of chondrocytes treaed with IGF Ⅰ at 100ng/ml was determined by flow cytometer.
Results:IGF Ⅰ stimulated proliferation and metabolism of chondrocytes at doses ranging from 10 to 100ng/ml.Maximal effect of stimulation occurred at 100ng/ml after treatment.The time of DNA synthesis(G1) was shorter in experimental group than in control group.]
Conclusion:IGF Ⅰ stimulated proliferation and metabolism of chondrocytes and shortened the time needed for G1 phase. |
| KEY WORDS Insulin like growth factor Ⅰ Joint diseases Cartilage |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 蓝旭,刘雪梅,葛宝丰,许建中.IGF-Ⅰ对培养兔关节软骨细胞作用的实验研究[J].中国骨伤,2001,14(6):341~342 |
| 英文格式: | LAN Xu,LIU Xue mei,GE Bao Feng.Effects of IGF-Ⅰ on proliferation and metabolism of cultured rabbit artiular chondrocytes[J].zhongguo gu shang / China J Orthop Trauma ,2001,14(6):341~342 |
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