| 持续劳损加冰敷法兔冻结肩模型的制备及评价 |
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Received:March 13, 2022
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| 作者 | Author | 单位 | Unit | E-Mail |
| 刘璐 |
LIU Lu |
上海市光华中西医结合医院, 上海 200052
上海中医药大学, 上海 201203 |
Shanghai Guanghua Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai 200052, China
Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China |
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| 程少丹 |
CHENG Shao-dan |
上海市光华中西医结合医院, 上海 200052
上海市中医药研究院中西医结合关节炎研究所, 上海 200052 |
Shanghai Guanghua Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai 200052, China
Arthritis Institute of Integrated Traditional Chinese and Western Medicine, Shanghai 200052, China |
chengshaodannew@126.com |
| 程杨 |
CHENG Yang |
上海市光华中西医结合医院, 上海 200052
上海市中医药研究院中西医结合关节炎研究所, 上海 200052 |
Shanghai Guanghua Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai 200052, China
Arthritis Institute of Integrated Traditional Chinese and Western Medicine, Shanghai 200052, China |
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| 彭思琛 |
PENG Si-chen |
上海市光华中西医结合医院, 上海 200052
上海中医药大学, 上海 201203 |
Shanghai Guanghua Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai 200052, China
Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China |
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| 葛程 |
GE Cheng |
上海市光华中西医结合医院, 上海 200052
上海市中医药研究院中西医结合关节炎研究所, 上海 200052 |
Shanghai Guanghua Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai 200052, China
Arthritis Institute of Integrated Traditional Chinese and Western Medicine, Shanghai 200052, China |
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| 王世辉 |
WANG Shi-hui |
上海市光华中西医结合医院, 上海 200052
上海市中医药研究院中西医结合关节炎研究所, 上海 200052 |
Shanghai Guanghua Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai 200052, China
Arthritis Institute of Integrated Traditional Chinese and Western Medicine, Shanghai 200052, China |
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| 期刊信息：《中国骨伤》2024年37卷，第4期，第392-398页 |
| DOI：10.12200/j.issn.1003-0034.20221303 |
| 基金项目:上海市科委课题(编号:19401935000,20Y21901600);上海市长宁区科委课题(编号:CNKW20185Y12);上海市长宁区医学硕博士创新人才基地项目(编号:RCJD2022S03);上海市长宁区名中医建设项目(编号:CNMZY-2021013);上海市长宁区青年中医医师培养项目(编号:2021CNQNZY002) |
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目的: 评估持续劳损加冰敷法制备兔冻结肩模型的成模情况。
方法: 选取质量为(2 500±500) g的清洁级、健康雄性新西兰兔12只,随机分为空白组和模型组,每组6只。模型组采用持续劳损加冰敷造模,观察兔大体情况及肩关节主被动活动情况,并记录体重。分别于造模后第6 天和第29 天对兔造模侧肩关节行MRI检查,观察积液及软组织情况;采用HE染色法观察兔肱二头肌长头肌腱及关节囊滑膜的形态;采用Masson染色法观察兔肱二头肌长头肌腱及关节囊滑膜的纤维沉积情况,并用Image J软件对纤维沉积行半定量分析。
结果: 造模结束6 d后,模型组兔肩关节主动活动受限,被动活动受限不明显,行走跛行;造模结束29 d后,模型组兔肩关节主、被动活动均严重受限;与空白组体重(2.50±0.14) kg相比,模型组体重(2.20±0.17) kg显著降低(P<0.01)。MRI显示,造模结束后6 d,肩周肌肉外形不光滑,关节囊结构模糊,关节腔内见大量积液;造模结束后29 d,肩周肌肉外形毛糙,关节囊结构不清,关节腔内积液较造模后6 d减少。病理染色显示,模型组兔肱二头肌长头肌腱肌纤维排列紊乱,形态蜷曲,甚至断裂,关节囊滑膜组织大量血管增生,胶原纤维沉积及严重炎性细胞浸润。通过Image J软件对Masson染色结果半定量分析,模型组造模后7、30 d兔患肩肱二头肌长头肌腱纤维沉积[(23.58±3.41) %、(27.56±3.70) %]及滑膜组织纤维沉积[(41.78±5.59) %、(62.19±7.54) %]均显著高于空白组肱二头肌长头肌腱纤维沉积[(1.79±1.03) %、(1.29±0.63) %]及滑膜组织纤维沉积[(8.15±3.61) %、(11.29±7.10) %](P<0.01),且时间越长,纤维化越严重(P<0.01)。
结论: 行为学、影像学和病理学均显示,持续劳损加冰敷法兔冻结肩模型符合冻结肩临床表现,切合冻结肩发病机制,是一种理想的冻结肩模型。 |
| [关键词]:冻结肩 模型 滑膜 |
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| Establishment and evaluation of a rabbit model of frozen shoulder induced by persistent strain injuries and ice compression |
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Abstract:
Objective To evaluate the rabbit modle of frozen shoulder induced by persistent strain injuries and ice compression.
Methods Twelve clean,healthy male New Zealand rabbits with a mass of (2 500±500) g were selected and randomly divided into a blank group and a control group with 6 rabbits in each group. In the control group,the rabbits were modeled with persistent strain injuries and ice compression,the general conditions of the rabbits and the active and passive activities of the shoulder joint were observed and their body weights were recorded. MRI was performed on the affected shoulder joints at 6 d and 29 d after modelling to observe the fluid and soft tissue;HE staining was used to observe the morphology of the rabbit biceps longus tendon and the synovial membrane of the joint capsule;Masson staining was used to observe the fibrous deposits of the rabbit biceps longus tendon and the synovial membrane of the joint capsule,and the fibrous deposits were analysed semi-quantitatively by Image J software.
Results Six days after the end of modeling,the active movement of the shoulder joints in the control group was limited,the passive movement was not significantly limited,and they walked with a limp;29 days after the end of the modeling,the active and passive movements of the shoulder joints in the model group were severely limited. Compared with the blank group (2.50±0.14) kg,the body weight of the model group (2.20±0.17) kg was significantly reduced(P<0.01). MRI showed that 6 days after modelling,the muscles around the shoulder joint were not smooth in shape,the joint capsule structure was narrowed and a large amount of fluid was seen in the joint cavity;29 days after modelling,the muscles around the shoulder joint were rough in shape,structure of the joint capsule was unclear and the fluid in the joint cavity was reduced compared with 6 days after modelling. Pathological staining showed that the long-headed biceps tendon fibres in the control group were disorganised,curled or even broken,and the synovial tissue of the joint capsule was heavily vascularised,with collagen fibre deposits and severe inflammatory cell infiltration. The fiber deposition of the long head of biceps brachii in the model group[(23.58±3.41) %,(27.56±3.70) %] and synovial tissue[(41.78±5.59) %,(62.19±7.54) %] were significantly higher than those in the blank group[(1.79±1.03) %,(1.29±0.63) %] at 7 and 30 days after modeling and synovial tissue fiber deposition[(8.15±3.61) %,(11.29±7.10) %],as shown by the semi-quantitative analysis of Masson staining results by Image J software. And the longer the time,the more severe the fibrosis (P<0.01).
Conclusion The behavioral,imaging and pathological findings showed that the rabbit frozen shoulder model with persistent strain injuries and ice compression is consistent with the clinical manifestations and pathogenesis of periarthritis,making it an ideal method for periarthritis research. |
| KEYWORDS:Frozen shoulder Model Synovial membrane |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 刘璐,程少丹,程杨,彭思琛,葛程,王世辉.持续劳损加冰敷法兔冻结肩模型的制备及评价[J].中国骨伤,2024,37(4):392~398 |
| 英文格式: | LIU Lu,CHENG Shao-dan,CHENG Yang,PENG Si-chen,GE Cheng,WANG Shi-hui.Establishment and evaluation of a rabbit model of frozen shoulder induced by persistent strain injuries and ice compression[J].zhongguo gu shang / China J Orthop Trauma ,2024,37(4):392~398 |
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