| 妥布霉素激活Wnt/β-catenin信号通路上调ALP和RUNX2蛋白表达促进骨折愈合的实验研究 |
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Received:November 06, 2020
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| 期刊信息：《中国骨伤》2021年34卷，第9期，第866-869页 |
| DOI：10.12200/j.issn.1003-0034.2021.09.015 |
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目的：探究妥布霉素（tobramycin，TOB）对大鼠股骨骨折愈合的影响。
方法：选取32只4~6周龄清洁级雄性SD大鼠，体重200~220 g，分为假手术组（A组）、骨折组（B组）、骨折+TOB组（C组）和骨折+TOB+IWR-1组（D组），每组8只。B、C、D组大鼠经闭合性股骨骨折造模法建立骨折模型；A组为假手术组，不做处理；D组大鼠于造模前1 d腹腔注射100 μl （8 μM） Wnt通路抑制剂（IWR-1-endo，IWR-1）。造模1 d后，C、D两组大鼠腹腔注射100 μl （100 μM） TOB，每天1次，共7 d。造模7周后，拍摄X线片观察B、C、D组大鼠的骨折愈合情况；Western-blotting法检测4组大鼠碱性磷酸酶（alkaline phosphatase，ALP），Runt相关转录因子2（Runt-related transcription factor 2，RUNX2）和Wnt信号通路中β-catenin蛋白的表达。
结果：X线片结果显示，C组与造模初期相比，骨折线消失，骨痂形成和骨折愈合良好；B、D两组仍可见少量骨折线，骨痂形成和骨折愈合不良。Western-blotting结果显示，B、C、D组的ALP、RUNX2、β-catenin蛋白表达均高于A组（P<0.05）；C组ALP、RUNX2、β-catenin蛋白表达水平较B、D两组升高（P<0.05）；应用通路抑制剂IWR-1后，D组ALP、RUNX2、β-catenin蛋白与B组比较差异无统计学意义（P>0.05）。
结论：妥布霉素可以通过激活Wnt/β-catenin信号通路，上调ALP、RUNX2蛋白的表达，促进成骨细胞的分化，进而促进骨折愈合。 |
| [关键词]:妥布霉素 成骨 骨折愈合 Wnt/β-catenin信号通路 |
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| Tobramycin promotes fracture healing by upregulating expressions of ALP and RUNX2 proteins through activating Wnt/β-catenin pathway |
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Abstract:
Objective: To explore effect of tobramycin (TOB) on healing of femoral fractures in rats.
Methods: Totally 32 male sprague-dawley (SD) rats were selected and randomly divided into sham group (group A),fracture group (group B),fracture with TOB group (group C) and fracture + TOB + IWR-1 group (group D),8 rats in each group. Close femoral fracture model in rats were established in group B,C and D,group A was sham operation without otherwise process. Group D was intraperitoneal injected 100 μl (8 μM) of Wnt pathway inhibitor IWR-1-endo (IWR-1) before molding at 1 day. At 1 day after molding,100 μl (100 μM) of TOB was intraperitoneally injected into group C and D at once a day for 7 days. At 7 weeks after modling,fracture healing of group B,C and D were observed by X-ray,Western-blotting was appilied to detect alkaline phosphatase(ALP) and Runt-related transcription factor 2 (RUNX2) and β-catenin of Wnt passway.
Results: X-ray results showed fracture line disappeared,callus formation and fracture healing well in group C compared with begning of molding; while a little fracture line,callus formation and fracture malunion in group B and d could be seen. Western-blotting results showed ALP,RUNX2 and expression of β-catenin in group B,C and D were higher than that of group A (P<0.05),while ALP,RUNX2 and β-catenin expression in group C was significantly higher than that of group B and D (P<0.05). After administration of pathway inhibitor IWR-1,there were no significance difference in ALP,RUNX2 and β-catenin protein expression between group D and group B(P>0.05).
Conclusion: Tobramycin could promote osteoblast differentiation and fracture healing by stimulating Wnt/β-catenin signaling pathway,up regulating expression of ALP and RUNX2. |
| KEYWORDS:Tobramycin Osteogenesis Fracture healing Wnt/β-catenin signaling pathway |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 崔宏刚.妥布霉素激活Wnt/β-catenin信号通路上调ALP和RUNX2蛋白表达促进骨折愈合的实验研究[J].中国骨伤,2021,34(9):866~869 |
| 英文格式: | CUI Hong-gang.Tobramycin promotes fracture healing by upregulating expressions of ALP and RUNX2 proteins through activating Wnt/β-catenin pathway[J].zhongguo gu shang / China J Orthop Trauma ,2021,34(9):866~869 |
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