| 韧带组织工程中物理环境影响干细胞分化的研究进展 |
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Received:October 20, 2019
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| 作者 | Author | 单位 | Unit | E-Mail |
| 张亚强 |
ZHANG Ya-qiang |
解放军联勤保障部队第940医院骨科中心, 甘肃 兰州 730050
兰州大学第二临床医院, 甘肃 兰州 730030 |
The 940 th Hospital of Joint Logistics Support Force of Chinese People's Liberation Army, Lanzhou 730050, Gansu, China |
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| 杨成伟 |
YANG Cheng-wei |
解放军联勤保障部队第940医院骨科中心, 甘肃 兰州 730050 |
The 940 th Hospital of Joint Logistics Support Force of Chinese People's Liberation Army, Lanzhou 730050, Gansu, China |
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| 封国超 |
FENG Guo-chao |
解放军联勤保障部队第940医院骨科中心, 甘肃 兰州 730050 |
The 940 th Hospital of Joint Logistics Support Force of Chinese People's Liberation Army, Lanzhou 730050, Gansu, China |
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| 李闯兵 |
LI Chuang-bing |
解放军联勤保障部队第940医院骨科中心, 甘肃 兰州 730050 |
The 940 th Hospital of Joint Logistics Support Force of Chinese People's Liberation Army, Lanzhou 730050, Gansu, China |
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| 时培晟 |
SHI Pei-sheng |
解放军联勤保障部队第940医院骨科中心, 甘肃 兰州 730050 |
The 940 th Hospital of Joint Logistics Support Force of Chinese People's Liberation Army, Lanzhou 730050, Gansu, China |
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| 甄平 |
ZHEN Ping |
解放军联勤保障部队第940医院骨科中心, 甘肃 兰州 730050 |
The 940 th Hospital of Joint Logistics Support Force of Chinese People's Liberation Army, Lanzhou 730050, Gansu, China |
Zhenpinggok@163.com |
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| 期刊信息：《中国骨伤》2020年33卷，第11期，第1080-1084页 |
| DOI：10.12200/j.issn.1003-0034.2020.11.019 |
| 基金项目:国家自然科学基金（编号：81601905，81371983）；全军后勤科研面上项目（编号：CWH17J007） |
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| 韧带组织工程是目前治疗韧带损伤的一种新型的方法，其可以替代自体移植物的不足。韧带组织工程包括4个基本要素：种子细胞、纳米支架、生长因子、机械刺激。目前韧带组织工程存在的主要问题是如何更可控的使种子细胞向韧带细胞分化。研究发现天然生物韧带的每一个物理特性及机械刺刺激（单轴拉伸）在干细胞向韧带细胞分化中起十分重要的作用，所以纳米纤维支架的设计必须要考虑材料的弹性模量、材料的结构（材料的排列方式、孔隙率及直径等），不同范围内的弹性模量及材料结构会引导细胞向不同谱系分化。考虑到韧带是人体主要的受力组织，所以机械刺激对干细胞分化也是必不可少的，尤其是单轴拉伸，其最符合韧带在体内的受力情况，大量研究发现拉伸的频率、幅度及时间也会引导细胞向不同方向分化。RhoA/卷曲螺旋蛋白激酶对细胞骨架的重构和分化起调节作用，同时研究发现RhoA/卷曲螺旋蛋白激酶蛋白参与了纳米纤维排布及机单轴拉伸引导干细胞向韧带细胞分化的过程，具体是如何影响干细胞分化的，目前并不清楚，了解物理特性对干细胞分化的影响并且弄清RhoA/卷曲螺旋蛋白激酶蛋白的作用机制，将为进一步优化韧带组织工程提供了新的理论依据。 |
| [关键词]:韧带组织工程 机械刺激 弹性模量 单轴拉伸 |
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| Advances on research of physical environment affecting stem cell differentiation in ligament tissue engineering |
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| Abstract:Ligament tissue engineering is currently a novel approach to the treatment of ligament injury,which can replace the deficiency of autografts. Ligament tissue engineering consists of four basic elements:seed cells,nanoscaffolds,growth factors,and mechanical stimulation. At present,the main problem in ligament tissue engineering is how to control seed cells to ligament cells more controllly. The study found that each physical property of the natural bio-ligament and mechanical stimulation(uniaxial stretching) plays an important role in the differentiation of stem cells into ligament cells. Therefore,the design of nanofiber scaffolds must consider the elastic modulus of the material and the material. Structure(material arrangement,porosity and diameter,etc.),elastic modulus and material structure in different ranges will guide cells to differentiate into different lineages. Considering that the ligament is the main force-bearing tissue of the human body,mechanical stimulation is also essential for stem cell differentiation,especially uniaxial stretching,which best meets the stress of the ligament in the body. A large number of studies have found the frequency and amplitude of stretching. And time will also lead the cells to differentiate in different directions. RhoA/ROCK plays a regulatory role in cytoskeletal remodeling and cell differentiation. It is also found that RhoA/ROCK protein participates in the process of nanofiber arrangement and uniaxial stretching to guide stem cells to differentiate into ligament cells,specifically how to influence stem cell differentiation. It is not clear at present that understanding the effects of physical properties on stem cell differentiation and understanding the mechanism of action of RhoA/ROCK protein will provide a new theoretical basis for further optimization of ligament tissue engineering. |
| KEYWORDS:Ligament tissue engineering Mechanical stimulation Elastic modulus Uniaxial stretching |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 张亚强,杨成伟,封国超,李闯兵,时培晟,甄平.韧带组织工程中物理环境影响干细胞分化的研究进展[J].中国骨伤,2020,33(11):1080~1084 |
| 英文格式: | ZHANG Ya-qiang,YANG Cheng-wei,FENG Guo-chao,LI Chuang-bing,SHI Pei-sheng,ZHEN Ping.Advances on research of physical environment affecting stem cell differentiation in ligament tissue engineering[J].zhongguo gu shang / China J Orthop Trauma ,2020,33(11):1080~1084 |
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