| 通络止痛凝胶制剂对KOA滑膜组织中p53/miR-502-5p/NF-κBp65的影响 |
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Received:May 15, 2019
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| 作者 | Author | 单位 | Unit | E-Mail |
| 张晓哲 |
ZHANG Xiao-zhe |
北京中医药大学第三附属医院, 北京 100029 |
The Third Affiliated Hospital of Beijing University of Chinese Medicine, Beijing 100029, China |
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| 张栋 |
ZHANG Dong |
北京中医药大学第三附属医院, 北京 100029 |
The Third Affiliated Hospital of Beijing University of Chinese Medicine, Beijing 100029, China |
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| 阮安民 |
RUAN An-min |
北京中医药大学第三附属医院, 北京 100029 |
The Third Affiliated Hospital of Beijing University of Chinese Medicine, Beijing 100029, China |
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| 李惠娟 |
LI Hui-juan |
北京中医药大学第三附属医院, 北京 100029 |
The Third Affiliated Hospital of Beijing University of Chinese Medicine, Beijing 100029, China |
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| 陈谱 |
CHEN Pu |
北京中医药大学第三附属医院, 北京 100029 |
The Third Affiliated Hospital of Beijing University of Chinese Medicine, Beijing 100029, China |
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| 周俊 |
ZHOU Jun |
北京中医药大学第三附属医院, 北京 100029 |
The Third Affiliated Hospital of Beijing University of Chinese Medicine, Beijing 100029, China |
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| 马玉峰 |
MA Yu-feng |
北京中医药大学第三附属医院, 北京 100029 |
The Third Affiliated Hospital of Beijing University of Chinese Medicine, Beijing 100029, China |
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| 祁印泽 |
QI Yin-ze |
北京中医药大学第三附属医院, 北京 100029 |
The Third Affiliated Hospital of Beijing University of Chinese Medicine, Beijing 100029, China |
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| 王庆甫 |
WANG Qing-fu |
北京中医药大学第三附属医院, 北京 100029 |
The Third Affiliated Hospital of Beijing University of Chinese Medicine, Beijing 100029, China |
qingpu-wang@sohu.com |
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| 期刊信息：《中国骨伤》2020年33卷，第7期，第643-648页 |
| DOI：10.12200/j.issn.1003-0034.2020.07.010 |
| 基金项目:国家自然科学基金面上项目（编号：81373662）；北京中医药大学自主课题项目（编号：2018-JYBZZ-XS236） |
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目的： 观察通络止痛凝胶制剂对膝骨关节炎（knee osteoarthritis，KOA）滑膜组织中p53、miR-502-5p、NF-κBp65的影响，探讨通络止痛凝胶制剂治疗KOA的作用机制。
方法： 选取8周龄Wistar大鼠36只，体重200~220 g，平均208 g，采用随机数字表法分为正常组、模型组、中药组，每组12只。采用改良Hulth法建立KOA模型，造模4周后，中药组给予通络止痛凝胶制剂外擦，3次/天，共2周；正常组和模型组正常饲养、不干预。治疗结束后，观察各组标本形态学变化；采用qPCR检测滑膜组织miR-502-5p的变化，再分别采用qPCR、Western Blot法检测滑膜组织p53、NF-κBp65、IL-1β、TNF-α、MMP-13的含量。
结果： （1）标本形态学观察结果，模型组关节软骨透明度下降、表面不平整，滑膜肥厚增生并有大量炎性细胞浸润，关节液质地较黏稠；中药组关节软骨透亮度下降不明显，关节面较平整，滑膜轻度增生并有少量炎性细胞浸润，关节液质地较清晰。（2）与正常组比较，模型组、中药组滑膜组织miR-502-5p含量升高（P<0.05），p53的mRNA及蛋白表达下降（P<0.05），NF-κBp65、IL-1β、TNF-α、MMP-13的mRNA及蛋白表达升高（P<0.05）。（3）与模型组比较，中药组滑膜组织miR-502-5p含量降低（P<0.05），p53的mRNA及蛋白表达升高（P<0.05），NF-κBp65、IL-1β、TNF-α、MMP-13的mRNA及蛋白表达降低（P<0.05）。
结论： 滑膜组织中p53、miR-502-5p、NF-kBp65的表达与滑膜的增生及炎症反应密切相关，通络止痛凝胶制剂可能是通过调控滑膜组织中p53、miR-502-5p、NF-κBp65的表达，从而减轻KOA滑膜的增生及炎症反应。 |
| [关键词]:骨关节炎,膝 滑膜 凝胶类 基因表达 |
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| Effect of Tongluo Zhitong(通络止痛,TLZT) gel preparation on p53/miR-502-5p/NF-κBp65 in synovial tissue of knee osteoarthritis |
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Abstract:
Objective: To observe effects of Tongluo Zhitong(通络止痛,TLZT) gel preparation on p53,miR-502-5p,NF-κBp65 in synovial tissue of knee osteoarthritis(KOA),and to explore mechanism of TLZT gel preparation in treating KOA.
Methods: Thirthy-six Wistar rats aged 8 weeks and weighed 200 to 220 g(meaned 208 g) were randomly divided into normal group,model group and traditional Chinese medicine(TCM) group,12 rats in each group. KOA model was established by modified Hulth method. After 4 weeks of modeling,TCM group treated with TLZT gel preparation for external use,3 times daily for 2 weeks;normal group and model group were fed normally without intervention. After treatment,morphological changes of specimens in each group were observed,changes of miR-502-5p in synovial tissue were detected by qPCR,and contents of p53,NF-κBp65,IL-1β,TNF-α,MMP-13 in synovial tissue were detected by qPCR and Western Blot respectively.
Results: (1)Morphological observation of specimens showed that the articular cartilage in model group was hyaline and uneven,the synovial membranes were hypertrophic and proliferative with a large number of inflammatory cells infiltrating,the joint fluid was thicker in texture;the articular cartilage in TCM group was more transparent and smooth,synovial hyperplasia was mild with a small amount of inflammatory cell infiltration,the texture of articular fluid was clear and sparse. (2) Compared with normal group,content of miR-502-5p of synovial tissue in model and TCM group were increased,mRNA and expression of p53 decreased,expression of NF-κBp65,IL-1β,TNF-α,MMP-13 increased.(3)Compared with model group,content of miR-502-5p in synovial tissue of TCM group decreased (P<0.05),mRNA and protein expression of p53 increased (P<0.05),mRNA and protein expression of NF-κBp65,IL-1β,TNF-α,MMP-13 decreased(P<0.05).
Conclusion: Expression of p53,miR-502-5p,NF-κBp65 in synovial tissue is closely related to synovial hyperplasia and inflammatory reaction,TLZT gel preparation may reduce proliferation and inflammatory reaction of KOA synovium by regulating the expression of p53,miR-502-5p,NF-κBp65 in synovial tissues. |
| KEYWORDS:Osteoarthritis,knee Synovial membrane Gels Gene expression |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 张晓哲,张栋,阮安民,李惠娟,陈谱,周俊,马玉峰,祁印泽,王庆甫.通络止痛凝胶制剂对KOA滑膜组织中p53/miR-502-5p/NF-κBp65的影响[J].中国骨伤,2020,33(7):643~648 |
| 英文格式: | ZHANG Xiao-zhe,ZHANG Dong,RUAN An-min,LI Hui-juan,CHEN Pu,ZHOU Jun,MA Yu-feng,QI Yin-ze,WANG Qing-fu.Effect of Tongluo Zhitong(通络止痛,TLZT) gel preparation on p53/miR-502-5p/NF-κBp65 in synovial tissue of knee osteoarthritis[J].zhongguo gu shang / China J Orthop Trauma ,2020,33(7):643~648 |
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