| 小针刀通过足三里对肌痛共病抑郁大鼠行为学及海马NLRP3和IL-1β表达的影响 |
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Received:June 20, 2019
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| 作者 | Author | 单位 | Unit | E-Mail |
| 王永志 |
WANG Yong-zhi |
首都医科大学附属北京友谊医院中医科, 北京 100050 |
Department of Traditional Chinese Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China |
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| 王安娜 |
WANG An-na |
首都医科大学附属北京友谊医院中医科, 北京 100050 |
Department of Traditional Chinese Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China |
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| 高雪松 |
GAO Xue-song |
北京市隆福医院康复医学科北苑院区, 北京 100010 |
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| 杜仪 |
DU Yi |
首都医科大学附属北京友谊医院中医科, 北京 100050 |
Department of Traditional Chinese Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China |
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| 李丽 |
LI Li |
首都医科大学附属北京友谊医院国际医学部, 北京 100050 |
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| 赵静洁 |
ZHAO Jing-jie |
首都医科大学附属北京友谊医院中医科, 北京 100050 |
Department of Traditional Chinese Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China |
13466376389@163.com |
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| 期刊信息：《中国骨伤》2019年32卷，第12期，第1151-1155页 |
| DOI：10.3969/j.issn.1003-0034.2019.12.017 |
| 基金项目:北京市自然科学基金资助项目（编号：7172063）；北京市卫生系统高层次卫生技术人才培养项目（编号：2014-3-001） |
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目的：观察小针刀刺激足三里穴对肌痛共病抑郁大鼠行为学及海马区Nod样受体蛋白3（Nod-likereceptor protein，NLRP3）和白介素1β（interleukin-1β，IL-1β）表达的影响。
方法：采用急性利血平腹腔注射法制备肌痛共病抑郁大鼠动物模型，将24只SD雄性大鼠随机分为正常组、模型组、小针刀组和阿米替林组，每组6只，分别给予对应处理。检测各组大鼠Open field行为学以及机械痛敏程度，智能热板仪检测各组大鼠热缩足阈值，应用Western blotting免疫印迹法检测大鼠海马NLRP3和IL-1β表达量。
结果：与模型组比较，小针刀组大鼠足底机械痛阈明显改善（P<0.01）；与阿米替林组比较，针刀刺激足三里可显著提高大鼠热缩足阈值（P<0.05）。在Open field行为学大鼠水平运动距离及穿格次数比较中，小针刀组大鼠旷场水平运动总距离明显增加（P<0.05）；与模型组比较，大鼠穿格次数有增多趋势，但差异无统计学意义（P>0.05）。模型组大鼠海马体NLRP3和IL-1β表达均明显升高（P<0.05），小针刀组IL-1β表达明显降低（P<0.05）；针刺足三里可抑制大鼠海马NLRP3的表达，但与模型组比较，差异无统计学意义（P>0.05）。
结论：小针刀可以改善利血平导致的肌痛共病抑郁大鼠的病理状态，其作用机制可能与抑制中枢海马内NLRP3炎性小体和IL-1β的表达有关。 |
| [关键词]:小针刀 足三里 肌痛 抑郁 |
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| Effect of the small needle knife through the Zusanli(ST 36) on behavior and hippocampal expression of NLRP3 and IL-1β in myalgia comorbid depressed rats |
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Abstract:
Objective: To observe the effect of the small needle knife through the Zusanli(ST 36) on behavior and hippocampal expression of NLRP3 and IL-1β in myalgia comorbid depressed rats.
Methods: The rat models of myalgia comorbid depression were prepared by intraperitoneal injection of acute reserpine. Twenty-four SD male rats were randomly divided into control group,model group,small needle knife group and amitriptyline group,6 rats in each group. The open field behavior and mechanical pain threshold of each group were detected. The thermal pain threshold was detected by intelligent hot plate test. The expression of NLRP3 and IL-1β in hippocampus of rats was detected by Western blotting.
Results: Compared with the model group,the mechanical pain threshold of the foot was significantly improved in the small needle knife group (P<0.01). Compared with the amitriptyline group,the small needle knife stimulation of Zusanli(ST 36) can significantly increase the thermal pain threshold in rats(P<0.05);in the comparison of the horizontal movement distance and the number of crossings in the open field behavioral rats,the total distance of the open field activity of the small needle knife group was significantly increased(P<0.05). Compared with the model group,the number of crossings in the small needle knife group had no statistically significant difference (P>0.05). The expressions of NLRP3 and IL-1β in the hippocampus of the model group were significantly increased(P<0.05),and the expression of IL-1β in the small needle knife group was significantly decreased (P<0.05). The stimulation of small needle knife at Zusanli(ST 36) could inhibit the expression of NLRP3 in hippocampus of rats. However,there was no statistically significant difference compared with the model group (P>0.05).
Conclusion: Small needle knife can improve the pathological state of myalgia comorbid depression caused by reserpine in rats. The mechanism may be related to the inhibition of NLRP3 inflammasome and IL-1β expression in central hippocampus. |
| KEYWORDS:Small needle knife Zusanli Myalgia Depression |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 王永志,王安娜,高雪松,杜仪,李丽,赵静洁.小针刀通过足三里对肌痛共病抑郁大鼠行为学及海马NLRP3和IL-1β表达的影响[J].中国骨伤,2019,32(12):1151~1155 |
| 英文格式: | WANG Yong-zhi,WANG An-na,GAO Xue-song,DU Yi,LI Li,ZHAO Jing-jie.Effect of the small needle knife through the Zusanli(ST 36) on behavior and hippocampal expression of NLRP3 and IL-1β in myalgia comorbid depressed rats[J].zhongguo gu shang / China J Orthop Trauma ,2019,32(12):1151~1155 |
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