| 锂剂促进自噬保护受损神经细胞的研究 |
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Received:December 06, 2018
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| 作者 | Author | 单位 | Unit | E-Mail |
| 张舵 |
ZHANG Duo |
首都医科大学附属北京天坛医院骨科, 北京 100050 |
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| 寨旭 |
ZHAI Xu |
西安交通大学第二附属医院急诊科, 陕西 西安 710004 |
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| 王放 |
WANG Fang |
西安交通大学第二附属医院骨二科, 陕西 西安 710004 |
Department of Orthopaedics, the Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, Shaanxi, China |
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| 李晓会 |
LI Xiao-hui |
西安交通大学第二附属医院影像科, 陕西 西安 710004 |
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| 贺西京 |
HE Xi-jing |
西安交通大学第二附属医院骨二科, 陕西 西安 710004 |
Department of Orthopaedics, the Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, Shaanxi, China |
xijing_h@vip.tom.com |
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| 期刊信息：《中国骨伤》2019年32卷，第10期，第952-956页 |
| DOI：10.3969/j.issn.1003-0034.2019.10.016 |
| 基金项目:北京市优秀人才培养资助项目（编号：2017000021469G215）；首都医科大学校自然基金（编号：PYZ2017082）；北京天坛医院青年科研基金（编号：2016-YQN-14） |
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目的：研究锂剂能否通过促进细胞自噬发挥神经细胞保护作用。
方法：将SH-SY5Y神经细胞分为4组，包括对照组（正常换液），模型组（培养液含有200 μmol/L H2O2的培养液），锂剂组（培养液含200 μmol/L H2O2及1.0 mmol/L LiCl的培养液），3-MA干预组（培养液含200 μmol/L H2O2、1.0 mmol/L LiCl及5 mmol/L 3-MA的培养液）。培养6 h后进行MTT检测及Beclin 1、LC3b免疫组化染色，评估细胞存活情况及自噬水平。
结果：3-MA干预组细胞存活率较模型组降低（P<0.05），锂剂组细胞存活率较模型组提高（P<0.05）。经过3-MA干预后，细胞存活率较对照组、模型组及锂剂组均有所降低（P<0.05）。经过H2O2处理后，细胞Beclin 1、LC3b染色面积增大，染色加深。经锂剂处理后细胞Beclin 1、LC3b染色面积进一步增大，染色更深。3-MA干预组细胞Beclin 1、LC3b染色面积较对照组大、染色深，但细胞染色面积较模型组和锂剂组均小，且染色更为浅淡。
结论：锂剂能够促进受损神经细胞存活，促进自噬作用是锂剂发挥神经保护作用的机制之一。 |
| [关键词]:神经损伤 锂剂 神经细胞 自噬 SH-SY5Y细胞 |
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| Study of the neural protective effect of lithium on enhancement of autophagy in vitro |
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Abstract:
Objective:To investigate whether lithium can exert neuroprotective effects by promoting autophagy.
Methods:SH-SY5Y cells were divided into 4 groups,including control group(handled with normal culture solution),model group (handled with 200 μmol/L H2O2),lithium group (handled with 200 μmol/L H2O2 and 1.0 mmol/L LiCl medium),3-MA group (handled with 200 μmol/L H2O2,1.0 mmol/L LiCl and 5 mmol/L 3-MA). After 6 hours of culture,MTT assay and immunohistochemical staining of Beclin 1 and LC3b were performed to evaluate cell survival and autophagy.
Results:The cell survival rate of lithium group was significantly high than that of the model group(P<0.05),while the 3-MA group was lower(P<0.05). After 3-MA intervention,the cell survival rate was lower than that of control group,model group and lithium group(P<0.05). After H2O2 treatment,the staining area of Beclin 1 and LC3b was increased and the staining was deeper,and after LiCl handling,the staining area of Beclin 1 and LC3b was further increased and the staining was more deeper. The staining area of Beclin 1 and LC3b in 3-MA group was larger than that in control group,but was smaller than that in model group and lithium group,and the staining was lighter.
Conclusion:Lithium can promote the survival of damaged nerve cells,and inducing autophagy is probably one of the neuroprotective mechanisms of lithium. |
| KEYWORDS:Neurological damage Lithium Neural cells Autophagy SH-SY5Y cells |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 张舵,寨旭,王放,李晓会,贺西京.锂剂促进自噬保护受损神经细胞的研究[J].中国骨伤,2019,32(10):952~956 |
| 英文格式: | ZHANG Duo,ZHAI Xu,WANG Fang,LI Xiao-hui,HE Xi-jing.Study of the neural protective effect of lithium on enhancement of autophagy in vitro[J].zhongguo gu shang / China J Orthop Trauma ,2019,32(10):952~956 |
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