| bFGF与骨髓间充质干细胞联合应用对大鼠脊髓损伤的修复作用 |
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Received:October 17, 2018
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| 作者 | Author | 单位 | Unit | E-Mail |
| 黄兴锐 |
HUANG Xing-rui |
吴江区中医医院 吴江区第二人民医院骨科, 江苏 苏州 215221 |
Department of Orthopaedics, the Second People's Hospital of Wujiang District, Suzhou 215221, Jiangsu, China |
huangxingrui2008@163.com |
| 徐浩 |
XU Hao |
吴江区中医医院 吴江区第二人民医院骨科, 江苏 苏州 215221 |
Department of Orthopaedics, the Second People's Hospital of Wujiang District, Suzhou 215221, Jiangsu, China |
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| 张晔 |
ZHANG Ye |
苏州大学医学部博习临床解剖学研究所暨细胞神经生物学研究室, 江苏 苏州 215123 |
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| 蒋元斌 |
JIANG Yuan-bin |
吴江区中医医院 吴江区第二人民医院骨科, 江苏 苏州 215221 |
Department of Orthopaedics, the Second People's Hospital of Wujiang District, Suzhou 215221, Jiangsu, China |
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| 夏春林 |
XIA Chen-lin |
苏州大学医学部博习临床解剖学研究所暨细胞神经生物学研究室, 江苏 苏州 215123 |
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| 方姝晨 |
FANG Shu-chen |
吴江区中医医院 吴江区第二人民医院骨科, 江苏 苏州 215221 |
Department of Orthopaedics, the Second People's Hospital of Wujiang District, Suzhou 215221, Jiangsu, China |
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| 期刊信息：《中国骨伤》2019年32卷，第7期，第653-657页 |
| DOI：10.3969/j.issn.1003-0034.2019.07.013 |
| 基金项目:苏州吴江区卫计委"科教兴卫"项目（编号：WWK201623） |
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目的：观察骨髓间充质干细胞（BMSCs）与成纤维细胞生长因子（bFGF）联合移植对大鼠脊髓损伤的修复作用，并探讨其机制。
方法：利用血清培养技术获得SD大鼠BMSCs。80只健康雄性6周龄SD大鼠（重约240 g）随机分为4组，每组20只。假手术组行单纯椎板切除，不损伤脊髓，与其余3组同条件饲养。其余3组均采用左侧T9脊髓半切，建立脊髓损伤模型。制模9 d后进行损伤局部移植治疗，对照组损伤处植入吸附有生理盐水的明胶海绵；BMSCs移植组损伤处植入吸附有BMSCs的明胶海绵；bFGF+BMSCs移植组损伤处植入吸附有bFGF+BMSCs的明胶海绵。术后4、8周后Western blotting分析受损脊髓组织中NF-200、GFAP表达水平，Basso Beattie Bresnahan（BBB）运动功能评分量表评价大鼠的后肢功能恢复情况。
结果：术后4、8周后BMSCs移植组和bFGF+BMSCs移植组的（BBB）评分较对照组改善（P<0.05），且bFGF+BMSCs移植组与BMSCs移植组比较差异有统计学意义（P<0.05）；术后4、8周后NF200在对照组表达极少，在BMSCs移植组只有少量表达，而bFGF+BMSCs移植组呈高表达（P<0.05）。GFAP在对照组表达高，BMSCs移植组少量表达，bFGF+BMSCs移植组呈现低表达（P<0.05）。bFGF+BMSCs移植组与BMSCs移植组、对照组比较差异有统计学意义（P<0.05）。
结论：BMSCs与bFGF联合移植对大鼠脊髓损伤有修复作用，机制可能与其降低GFAP表达及升高NF-200表达有关。 |
| [关键词]:骨髓间充质干细胞 成纤维细胞生长因子 脊髓损伤 神经丝蛋白-200 胶质纤维酸性蛋白 |
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| Repair effect of bFGF combined with bone marrow mesenchymal stem cells on spinal cord injury in rats |
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Abstract:Objective:To observe the repair effect of bone marrow mesenchymal stem cells (BMSCs) combined with basic fibroblast growth factor (bFGF) on spinal cord injury in rats and explore its mechanism.
Methods:SD rat BMSCs were obtained by serum culture technique. Eighty healthy 6-week-old male SD rats(weight about 240 g) were randomly divided into 4 groups with 20 each. The sham operation group underwent simple laminectomy without damaging spinal cord and was kept in the same condition as the other 3 groups. The other 3 groups underwent left T9 spinal cord hemisection to establish spinal cord injury model. After 9 days of modeling the local transplantation was performed. The Control group was implanted with gelatin sponge containing normal saline. The BMSCs transplantation group was implanted with gelatin sponge containing BMSCs. The bFGF+BMSCs transplantation group was implanted with gelatin sponge containing bFGF+BMSCs. After 4 and 8 weeks,the expression of NF-200 and GFAP in injured spinal cord tissue was analyzed by Western blotting and the recovery of hind limb function was evaluated by Basso Beattie Bresnahan(BBB) motor function score scale.
Results:The BBB scores of BMSCs transplantation group and bFGF+BMSCs transplantation group were better than control group at 4 and 8 weeks after operation (P<0.05) and there was significant difference between bFGF+BMSCs transplantation group and BMSCs transplantation group (P<0.05). After 4 and 8 weeks postoperatively,NF-200 expression was minimal in control group and only a small amount was expressed in BMSCs transplantation group while in bFGF+BMSCs transplantation group NF-200 was highly expressed(P<0.05). GFAP expression was high in control group,middle in BMSCs transplantation group and low in bFGF BMSCs transplantation group(P<0.05). There was significant difference between bFGF+BMSCs transplantation group,BMSCs transplantation group and control group(P<0.05).
Conclusion:The combined transplantation of BMSCs and bFGF can repair the spinal cord injury in rats. The mechanism may be related to the decrease of GFAP expression and the increase of NF-200 expression. |
| KEYWORDS:Bone marrow mesenchymal stem cells Fibroblast growth factor Spinal cord injury Neurofilament protein-200 Glial fibrillary acidic protein |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 黄兴锐,徐浩,张晔,蒋元斌,夏春林,方姝晨.bFGF与骨髓间充质干细胞联合应用对大鼠脊髓损伤的修复作用[J].中国骨伤,2019,32(7):653~657 |
| 英文格式: | HUANG Xing-rui,XU Hao,ZHANG Ye,JIANG Yuan-bin,XIA Chen-lin,FANG Shu-chen.Repair effect of bFGF combined with bone marrow mesenchymal stem cells on spinal cord injury in rats[J].zhongguo gu shang / China J Orthop Trauma ,2019,32(7):653~657 |
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