| TLR4/NF-κB通路参与大鼠膝骨关节炎滑膜早期病变的研究 |
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Received:October 30, 2018
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| 作者 | Author | 单位 | Unit | E-Mail |
| 王学宗 |
WANG Xue-zong |
上海中医药大学附属曙光医院石氏伤科医学中心, 上海 201203
上海市中医药研究院骨伤科研究所, 上海 201203 |
Shi's Center of Orthopaedics and Traumatology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China |
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| 丁道芳 |
DING Dao-fang |
上海市中医药研究院骨伤科研究所, 上海 201203 |
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| 薛艳 |
XUE Yan |
上海中医药大学附属曙光医院石氏伤科医学中心, 上海 201203 |
Shi's Center of Orthopaedics and Traumatology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China |
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| 顾新丰 |
GU Xin-feng |
上海中医药大学附属曙光医院石氏伤科医学中心, 上海 201203 |
Shi's Center of Orthopaedics and Traumatology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China |
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| 庞坚 |
PANG Jian |
上海市中医药研究院骨伤科研究所, 上海 201203 |
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| 张旻 |
ZHANG Min |
上海市中医药研究院骨伤科研究所, 上海 201203 |
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| 郑昱新 |
ZHENG Yu-xin |
上海中医药大学附属曙光医院石氏伤科医学中心, 上海 201203 |
Shi's Center of Orthopaedics and Traumatology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China |
sg_zyx1728@126.com |
| 曹月龙 |
CAO Yue-long |
上海中医药大学附属曙光医院石氏伤科医学中心, 上海 201203
上海市中医药研究院骨伤科研究所, 上海 201203 |
Shi's Center of Orthopaedics and Traumatology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China |
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| 詹红生 |
ZHAN Hong-sheng |
上海中医药大学附属曙光医院石氏伤科医学中心, 上海 201203
上海市中医药研究院骨伤科研究所, 上海 201203 |
Shi's Center of Orthopaedics and Traumatology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China |
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| 期刊信息：《中国骨伤》2019年32卷，第1期，第68-71页 |
| DOI：10.3969/j.issn.1003-0034.2019.01.015 |
| 基金项目:国家自然科学基金（编号：81503598；81503592；81674003；81774340）；上海市进一步加快中医药事业发展三年行动计划（编号：ZY3-LCPT-2-1005） |
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目的：探讨TLR4/NF-κB通路在大鼠膝骨关节炎（OA）滑膜早期病变的表达情况。
方法：将8周龄体重为（200±20）g的18只雄性SD大鼠按照随机数字表法分为模型组和对照组，每组9只。模型组按照改良Hulth法构建膝OA模型，对照组不做手术。分别于术后4、21 d提取滑膜组织和血清，采用PCR法检测CD14、TLR-4、IL-1β、TNF-α、MMP-13、ADAMTS-4表达；采用Western-blot法检测NF-κB p65蛋白表达；采用Elisa法检测血清中透明质酸（HA）及Ⅲ型前胶原氨基端（PⅢNP）浓度。
结果：术后4、21 d时模型组CD14、ADAMTS-4及NF-κB p65表达均较对照组升高，术后21 d时模型组TLR-4、Ⅱ-1β、TNF-α及MMP-13表达较对照组升高（P<0.01）。模型组术后4 d时血清PⅢNP和HA浓度高于对照组，术后21 d时两组比较差异无统计学意义。
结论：NF-κB通路可通过CD14/TLR-4早期激活进而触发滑膜分泌炎性因子IL-1β、TNF-a、MMP-13、ADAMTS-4，PⅢNP和HA增加介导膝OA发生。 |
| [关键词]:骨关节炎,膝 滑膜 大鼠 |
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| Role of TLR4/NF-κB pathway for early change of synovial membrane in knee osteoarthritis rats |
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Abstract:
Objective: To study role of TLR4/NF-κB pathway for early change of synovial membrane in knee osteoarthritis rats.
Methods: Eighteen male SD rats weighted (200±20) g were randomly divided into 2 groups,namely control and model group,and 9 in each group. Knee OA model group was established by using modified Hulth method in model group. Control group was not treated. Synovial tissue and serum was extracted at 4 and 21 d after operation. Expression of CD14,TLR4,IL-1β,TNF-α,ADAMTS-4,MMP-13 were detected by real-time PCR respectively. NF-κB p65 protein was detected by Western-blot;serum concentrations of haluronic acid (HA),N-propeptide of type Ⅲ procollagen(PⅢNP) was detected by Elisa.
Results: Expression of CD14,ADAMTS-4,and NF-κB p65 in model group were higher than that of control group at 4 and 21 days after operation,while expression of TLR4,IL-1β,TNF-α and MMP-13 were higher than that of control group at 21 days after operation(P<0.01). Concentration of PⅢNP and HA in model group were higher than that of control group at 4 days after operation,while there was no significant difference at 21 days after operation.
Conclusion: NF-κB pathway could mediate occurrence of KOA by early activating and triggeringg synovial increasingly secreting inflammatory secretion CD14,TLR4,IL-1β,TNF-α,ADAMTS-4,MMP-13,PⅢNP and HA. |
| KEYWORDS:Osteoarthritis,knee Synovial membrane Rats |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 王学宗,丁道芳,薛艳,顾新丰,庞坚,张旻,郑昱新,曹月龙,詹红生.TLR4/NF-κB通路参与大鼠膝骨关节炎滑膜早期病变的研究[J].中国骨伤,2019,32(1):68~71 |
| 英文格式: | WANG Xue-zong,DING Dao-fang,XUE Yan,GU Xin-feng,PANG Jian,ZHANG Min,ZHENG Yu-xin,CAO Yue-long,ZHAN Hong-sheng.Role of TLR4/NF-κB pathway for early change of synovial membrane in knee osteoarthritis rats[J].zhongguo gu shang / China J Orthop Trauma ,2019,32(1):68~71 |
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