| 淫羊藿苷在大鼠脊髓损伤中的神经保护作用 |
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Received:May 08, 2018
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| 作者 | Author | 单位 | Unit | E-Mail |
| 任宪盛 |
REN Xian-sheng |
吉林大学第二医院骨科, 吉林 长春 130041 |
Department of Orthopaedics, the Second Hospital of Jilin University, Changchun 130041, Jilin, China |
antren@163.com |
| 丁巍 |
DING Wei |
吉林大学第二医院外科, 吉林 长春 130041 |
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| 杨小玉 |
YANG Xiao-yu |
吉林大学第二医院骨科, 吉林 长春 130041 |
Department of Orthopaedics, the Second Hospital of Jilin University, Changchun 130041, Jilin, China |
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| 期刊信息：《中国骨伤》2018年31卷，第11期，第1054-1060页 |
| DOI：10.3969/j.issn.1003-0034.2018.11.014 |
| 基金项目:国家自然科学基金资助项目（编号：31572217） |
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目的：研究淫羊藿苷在大鼠脊髓损伤中的神经保护作用。
方法：108只SPF级雄性3月龄SD大鼠按随机数字表法分为实验组、对照组及假手术组3组，每组36只。对照组和实验组采用改良Allen法制作脊髓损伤模型，假手术组仅切开椎板不损伤脊髓。术后即刻实验组给予淫羊藿苷（100 mg/kg）灌胃，对照组和假手术组给予等量生理盐水灌胃，每日2次。术后1、2、3 d采用BBB评分法评定大鼠运动功能；术后72 h采用分光光度法检测髓过氧化物酶（myeloperoxidase，MPO）的活性，酶联免疫吸附测定（ELISA）法检测肿瘤坏死因子（tumor necrosis factor，TNF）-α、白介素（interleukin，IL）-1β的含量，免疫组化染色检测MPO、TNF-α、IL-1β的表达；采用硫代巴比妥酸法检测丙二醛（malondialdehyde，MDA）含量，黄嘌呤氧化酶法检测超氧化物歧化酶（superoxide dismutase，SOD）活性；采用TUNEL法检测细胞凋亡并计算细胞凋亡指数（apoptosis index，AI）；光镜观察脊髓损伤后组织病理学的改变并行组织病理学评分。
结果：术后各时间点对照组和实验组大鼠BBB评分均显著低于假手术组（P<0.05）；术后2、3 d实验组大鼠BBB评分均显著高于对照组（P<0.05）。术后72 h，对照组和实验组MPO活性和TNF-α、IL-1β的含量显著高于假手术组（P<0.05）；实验组显著低于对照组（P<0.05）。对照组和实验组MPO、TNF-α、IL-1β的表达显著高于假手术组（P<0.05）；实验组显著低于对照组（P<0.05）。对照组和实验组MDA含量显著高于假手术组，实验组显著低于对照组（P<0.05）；对照组和实验组SOD活性显著低于假手术组，实验组显著高于对照组（P<0.05）。对照组和实验组脊髓组织中AI显著高于假手术组，实验组显著低于对照组（P<0.05）。对照组和实验组脊髓组织病理学评分均显著高于假手术组，实验组均显著低于对照组（P<0.05）。
结论：淫羊藿苷能够抑制脊髓损伤后的炎症、脂质过氧化和细胞凋亡，减轻脊髓组织病理学损伤，改善脊髓损伤大鼠的运动功能，有效保护脊髓组织，具有明显的神经保护作用。 |
| [关键词]:淫羊藿苷 脊髓损伤 神经保护 大鼠 |
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| Neuroprotective effect of icariin on spinal cord injury in rats |
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Abstract:
Objective: To study the neuroprotective effect of icariin on spinal cord injury in rats.
Methods: A total 108 SPF male 3-month-old SD rats were divided into experimental group, control group and sham operation group according to the random number table. There were 36 rats in each group. In the control group and the experimental group, the modified Allen's method was used to make the spinal cord injury model. In the sham operation group, only the lamina was cut without damaging the spinal cord. Immediately after operation, the experimental group was given intragastric administration of icariin (100 mg/kg), the control group and sham operation group were given an equal amount of normal saline by gavage, twice a day. BBB score was used to assess the motor function of rats on 1, 2, 3 days after operation. At 72 h after operation, the activity of myeloperoxidase (MPO) was measured by spectrophotometry. Tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) levels was detected by enzyme-linked immunosorbent assay (ELISA). MPO, TNF-α, IL-1β expression were detected by immunohistochemical staining. Malondialdehyde (MDA) content was detected by thiobarbituric acid method. Superoxide dismutase (SOD) activity was measured by xanthine oxidase method. TUNEL staining was used to detect the apoptosis and apoptosis index (AI) was calculated. The histopathological changes of the spinal cord were observed under a light microscope and the histopathological score was performed using Sirin score method.
Results: BBB score in the control group and the experimental group was significantly lower than that in the sham operation group at each postoperative time point (P<0.05). BBB score in the experimental group was significantly higher than that in the control group at 2 and 3 days after operation (P<0.05). At 72 h after operation, the MPO activity and the levels of TNF-α, IL-1β in the control group and experimental group were significantly higher than in the sham operation group (P<0.05), and the experimental group was obviously higher than control group (P<0.05). The expressions of MPO, TNF-α, IL-1β in the control group and experimental group were significantly higher than in the sham operation group (P<0.05), and the experimental group was significantly lower than of the control group (P<0.05). MDA content in the control group and the experimental group was significantly higher than that in the sham operation group, and the experimental group was significantly lower than that in the control group (P<0.05). SOD activity in the control group and the experimental group was significantly lower than that in the sham operation group, and the experimental group was significantly higher than that in the control group (P<0.05). The AI in the control group and the experimental group was significantly higher than that in the sham operation group, and the experimental group was significantly lower than that in the control group (P<0.05). The histopathological score in the control group and the experimental group was significantly higher than that in the sham operation group, and the experimental group was significantly lower than that in the control group (P<0.05).
Conclusion: Icariin can inhibit inflammation, lipid peroxidation and apoptosis after spinal cord injury, reduce histopathological damage of spinal cord, improve the motor function, effectively protect spinal cord tissue, and has an obvious neuroprotective effect. |
| KEYWORDS:Icariin Spinal cord injury Neuroprotection Rats |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 任宪盛,丁巍,杨小玉.淫羊藿苷在大鼠脊髓损伤中的神经保护作用[J].中国骨伤,2018,31(11):1054~1060 |
| 英文格式: | REN Xian-sheng,DING Wei,YANG Xiao-yu.Neuroprotective effect of icariin on spinal cord injury in rats[J].zhongguo gu shang / China J Orthop Trauma ,2018,31(11):1054~1060 |
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