| 50 Hz 1.8 mT正弦交变电磁场对生长期大鼠骨密度的影响研究 |
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Received:March 17, 2017
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| 作者 | Author | 单位 | Unit | E-Mail |
| 高玉海 |
GAO Yu-hai |
兰州军区总医院全军创伤骨科研究所, 甘肃 兰州 730050 |
Institute of Orthopaedics, Lanzhou General Hospital of Lanzhou Military Area Command of Chinese PLA, Lanzhou 730050, Gansu, China |
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| 周延峰 |
ZHOU Yan-feng |
兰州军区总医院全军创伤骨科研究所, 甘肃 兰州 730050 |
Institute of Orthopaedics, Lanzhou General Hospital of Lanzhou Military Area Command of Chinese PLA, Lanzhou 730050, Gansu, China |
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| 李少锋 |
LI Shao-feng |
兰州军区总医院全军创伤骨科研究所, 甘肃 兰州 730050 |
Institute of Orthopaedics, Lanzhou General Hospital of Lanzhou Military Area Command of Chinese PLA, Lanzhou 730050, Gansu, China |
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| 李文苑 |
LI Wen-yuan |
兰州军区总医院全军创伤骨科研究所, 甘肃 兰州 730050 |
Institute of Orthopaedics, Lanzhou General Hospital of Lanzhou Military Area Command of Chinese PLA, Lanzhou 730050, Gansu, China |
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| 葸慧荣 |
XI Hui-rong |
兰州军区总医院全军创伤骨科研究所, 甘肃 兰州 730050 |
Institute of Orthopaedics, Lanzhou General Hospital of Lanzhou Military Area Command of Chinese PLA, Lanzhou 730050, Gansu, China |
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| 杨芳芳 |
YANG Fang-fang |
兰州军区总医院全军创伤骨科研究所, 甘肃 兰州 730050 |
Institute of Orthopaedics, Lanzhou General Hospital of Lanzhou Military Area Command of Chinese PLA, Lanzhou 730050, Gansu, China |
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| 陈克明 |
CHEN Ke-ming |
兰州军区总医院全军创伤骨科研究所, 甘肃 兰州 730050 |
Institute of Orthopaedics, Lanzhou General Hospital of Lanzhou Military Area Command of Chinese PLA, Lanzhou 730050, Gansu, China |
chkeming@lut.cn |
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| 期刊信息：《中国骨伤》2017年30卷，第12期，第1113-1117页 |
| DOI：10.3969/j.issn.1003-0034.2017.12.008 |
| 基金项目:国家自然科学基金（编号：81270963）；国家国际科技合作专项项目（编号：2015DFR30940）；甘肃省空间辐射生物学重点实验室开放基金（编号：2016GKL002） |
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目的：探讨50 Hz 1.8 mT正弦交变电磁场（sinusoidal electromagnetic fields，SEMFs）对SD大鼠骨密度（bone mineral density，BMD）的影响。
方法：将30只1月龄体重为（110±10）g的SD大鼠随机分为正常对照组和电磁场组，各15只。分别给予对照组50 Hz 0 mT和电磁场组50 Hz 1.8 mT强度正弦交变电磁场干预，1.5 h/d，每周称取体重1次，每天监测进食量变化，6周后腹腔注射麻醉大鼠并用双能X骨密度检测全身骨密度，处死后检测股骨骨密度及椎体骨密度；ELISA法测定血清骨钙素（Osteocalcin，OC）和血清抗酒石酸性磷酸酶5b（tartrate-resistant acid phosphatase 5b，TRACP 5b）；剥离肝、肾、子宫称重，计算器官指数并做HE切片做常规病理学检测分析。
结果：与对照组相比，电磁场组大鼠各周体重、每日进食量未见明显变化；2、4周后全身骨密度无明显变化，6周后全身骨密度及离体股骨和椎骨骨密度显著增加；血清OC表达量显著增加，血清TRACP 5b表达量显著降低；子宫、肝脏、脾脏HE染色未见病变，脏器指数无异常。
结论：50 Hz 1.8 mT正弦交变电磁场能通过提高骨形成降低骨吸收相关因子的表达，从而提高青年大鼠的峰值骨密度，为临床电磁场预防骨质疏松的研究奠定基础。 |
| [关键词]:电磁场 骨密度 大鼠 |
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| Effect of 50 Hz 1.8 mT sinusoidal electromagnetic fields on bone mineral density in growing rats |
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Abstract:
Objective: To study effects of 50 Hz 1.8 mT sinusoidal electromagnetic fields (SEMFs) on bone mineral density (BMD) in SD rats.
Methods: Thirty SD rats weighted(110±10) and aged 1 month were randomly divided into control group and electromagnetic field group,15 in each group. Normal control group of 50 Hz 0 mT density and sinusoidal electromagnetic field group of 50 Hz 1.8 mT were performed respectively with 1.5 h/d and weighted weight once a week,and observed food-intake. Rats were anesthesia by intraperitoneal injection and dual energy X-ray absorptiometry were used to detect bone density of whole body,and detected bone density of femur and vertebral body. Osteocalcin and tartrate-resistant acid phosphatase 5b were detected by ELSA;weighted liver,kidney and uterus to calculate purtenance index,then detected pathologic results by HE.
Results: Compared with control group,there was no significant change in weight every week,food-intake every day; no obvious change of bone density of whole body at 2 and 4 weeks,however bone density of whole body,bone density of excised femur and vertebra were increased at 6 weeks. Expression of OC was increased,and TRACP 5b expression was decreased. No change of HE has been observed in liver,kidney and uterus and organic index.
Conclusion: 50 Hz 1.8 mT sinusoidal electromagnetic fields could improve bone formation to decrease relevant factors of bone absorbs,to improve peak bone density of young rats,in further provide a basis for clinical research electromagnetic fields preventing osteoporosis foundation. |
| KEYWORDS:Electromagnetic fields Bone density Rats |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 高玉海,周延峰,李少锋,李文苑,葸慧荣,杨芳芳,陈克明.50 Hz 1.8 mT正弦交变电磁场对生长期大鼠骨密度的影响研究[J].中国骨伤,2017,30(12):1113~1117 |
| 英文格式: | GAO Yu-hai,ZHOU Yan-feng,LI Shao-feng,LI Wen-yuan,XI Hui-rong,YANG Fang-fang,CHEN Ke-ming.Effect of 50 Hz 1.8 mT sinusoidal electromagnetic fields on bone mineral density in growing rats[J].zhongguo gu shang / China J Orthop Trauma ,2017,30(12):1113~1117 |
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