| 花生四烯酸与红细胞急性损伤的相关性研究 |
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Received:April 19, 2015
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| 作者 | Author | 单位 | Unit | E-Mail |
| 袁涛 |
YUAN Tao |
南京军区南京总医院骨科, 江苏 南京 210002 |
Department of Orthopaedics, Nanjing General Hospital of Nanjing Military Region of Chinese PLA, Nanjing 210002, Jiangsu, China |
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| 赵建宁 |
ZHAO Jian-ning |
南京军区南京总医院骨科, 江苏 南京 210002 |
Department of Orthopaedics, Nanjing General Hospital of Nanjing Military Region of Chinese PLA, Nanjing 210002, Jiangsu, China |
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| 孟嘉 |
MENG Jia |
南京军区南京总医院骨科, 江苏 南京 210002 |
Department of Orthopaedics, Nanjing General Hospital of Nanjing Military Region of Chinese PLA, Nanjing 210002, Jiangsu, China |
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| 丛宇 |
CONG Yu |
南京军区南京总医院骨科, 江苏 南京 210002 |
Department of Orthopaedics, Nanjing General Hospital of Nanjing Military Region of Chinese PLA, Nanjing 210002, Jiangsu, China |
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| 陈双双 |
CHEN Shuang-shuang |
中国人民解放军第455医院眼科, 江苏 南京 200050 |
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| 包倪荣 |
BAO Ni-rong |
南京军区南京总医院骨科, 江苏 南京 210002 |
Department of Orthopaedics, Nanjing General Hospital of Nanjing Military Region of Chinese PLA, Nanjing 210002, Jiangsu, China |
bnrbnr@sina.com |
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| 期刊信息：《中国骨伤》2016年29卷，第2期，第179-183页 |
| DOI：10.3969/j.issn.1003-0034.2016.02.019 |
| 基金项目:江苏省临床科技基金(编号:BL2012002);江苏省自然科技基金(编号:BK2012776) |
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目的:观察花生四烯酸对大鼠红细胞的影响,构建隐性失血动物模型,并探讨隐性失血的病理机制.
方法:将50只体重(200±20) g的健康成年雄性Sprague-Dawley大鼠随机分为5组:对照组和4个实验组,其中实验组从尾静脉分别给予0.5 ml浓度5、10、20、40 mmol/L的花生四烯酸稀释液,建立体内高花生四烯酸水平的动物模型,对照组予以等量的空白对照液.分别在给药前及给药24 、48、72 h后采集眼静脉血液样本,采用全自动血液分析仪检测血液样本中血红蛋白、红细胞计数含量变化情况.对发生隐性失血的实验组,进一步测量其谷胱甘肽过氧化物酶(glutathion peroxidase,GSH-PX)活力,总超氧化物歧化酶(total superoxide dismutase,T-SOD)活力,过氧化氢(hydrogen peroxide,H2O2)的含量变化.
结果:当花生四烯酸的给药浓度为10 mmol/L时,实验组大鼠即出现隐性失血.在给药24 h后,各组大鼠的血红蛋白、红细胞均不同程度下降,实验组与对照组相比差异有统计学意义;GSH-PX活力、T-SOD活力、H2O2含量也均下降,实验组变化更显着.给药48 h后,对照组大鼠体内血液中血红蛋白、红细胞含量变化相对稳定,实验组血红蛋白、红细胞、GSH-PX活力、T-SOD活力、H2O2含量持续下降,且实验组变化更显着.72 h后,实验组大鼠血液中各项指标平稳,且有不同程度回升.
结论:血液中高浓度的花生四烯酸可诱导氧化应激反应,从而引起血液中红细胞及血红蛋白的急性损伤,导致隐性失血. |
| [关键词]:花生四烯酸 红细胞 失血,手术 |
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| Association of red blood cell damage with arachidonic acid |
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Abstract:
Objective:To study the correlation between arachidonic acid(AA) and acute red blood cells damage in rats,and to build a model with hidden blood loss in vivo,and to explore the pathological mechenism of hidden blood loss.
Methods:A total of 50 male adult Sprague-Dawley rats weighing(200±20) g were randomly divided into five groups (n=10):control group and four experimental groups. The rats in the experimental groups were given 0.5 ml different concentrations of AA diluents,5,10,20,40 mmol/L respectively. The blood samples were collected from orbital venous at the beginning and 24,48,72 hours after administration. Then the changes of hemoglobin (Hb),red blood cell count (RBC),glutathione peroxidase (GSH-PX) activity,total superoxide dismutase (T-SOD) activity and hydrogen peroxide (H2O2) in the blood samples were tested.
Results:Significant hidden blood loss occurred when the concentration was 10 mmol/L in the experimental group,with the RBC and Hb sharply reduced in blood samples. The Hb and RBC were reduced in all the experimental groups and control group at 24 hours after administration,while in the experimental groups they changed more obviously. The GSH-PX activity,T-SOD activity and H2O2 were also significantly reduced in all groups,and the changes showed significant differences. The Hb and RBC were relatively stable in the control group and the experimental groups at 48 hours after administration;while GSH-PX activity,T-SOD activity and H2O2 were all significantly decreased,and the changes in the experimental groups were more notable.
Conclusion:Elevated levels of AA in the blood causes oxidative stress in the red blood cells,leading to the damage of red blood cells and hemoglobin,which is responsible for hidden blood loss. |
| KEYWORDS:Arachidonic acid Erythrocytes Blood loss,surgical |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 袁涛,赵建宁,孟嘉,丛宇,陈双双,包倪荣.花生四烯酸与红细胞急性损伤的相关性研究[J].中国骨伤,2016,29(2):179~183 |
| 英文格式: | YUAN Tao,ZHAO Jian-ning,MENG Jia,CONG Yu,CHEN Shuang-shuang,BAO Ni-rong.Association of red blood cell damage with arachidonic acid[J].zhongguo gu shang / China J Orthop Trauma ,2016,29(2):179~183 |
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