| 蛇床子素对体外培养大鼠股骨组织吸收的抑制作用 |
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Received:December 14, 2014
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| 作者 | Author | 单位 | Unit | E-Mail |
| 周建 |
ZHOU Jian |
中国人民解放军区兰州军区兰州总医院骨科研究所, 甘肃 兰州 730050 |
Institute of Orthopaedics, Lanzhou General Hospital of PLA, Lanzhou 730050, Gansu, China |
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| 任雪梅 |
REN Xue-mei |
中国人民解放军区兰州军区兰州总医院输血科, 甘肃 兰州 730050 |
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| 马小妮 |
MA Xiao-ni |
中国人民解放军区兰州军区兰州总医院骨科研究所, 甘肃 兰州 730050 |
Institute of Orthopaedics, Lanzhou General Hospital of PLA, Lanzhou 730050, Gansu, China |
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| 高玉海 |
GAO Yu-hai |
中国人民解放军区兰州军区兰州总医院骨科研究所, 甘肃 兰州 730050 |
Institute of Orthopaedics, Lanzhou General Hospital of PLA, Lanzhou 730050, Gansu, China |
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| 闫丽娟 |
YAN Li-juan |
中国人民解放军区兰州军区兰州总医院骨科研究所, 甘肃 兰州 730050 |
Institute of Orthopaedics, Lanzhou General Hospital of PLA, Lanzhou 730050, Gansu, China |
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| 石文贵 |
SHI Wen-gui |
中国人民解放军区兰州军区兰州总医院骨科研究所, 甘肃 兰州 730050 |
Institute of Orthopaedics, Lanzhou General Hospital of PLA, Lanzhou 730050, Gansu, China |
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| 陈克明 |
CHEN Ke-ming |
中国人民解放军区兰州军区兰州总医院骨科研究所, 甘肃 兰州 730050 |
Institute of Orthopaedics, Lanzhou General Hospital of PLA, Lanzhou 730050, Gansu, China |
chkeming@yahoo.com.cn |
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| 期刊信息：《中国骨伤》2015年28卷，第9期，第832-837页 |
| DOI：10.3969/j.issn.1003-0034.2015.09.012 |
| 基金项目:国家自然科学基金(编号:81270963) |
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目的:研究蛇床子素(Osthole,OS)对体外培养股骨组织(骨干和骨骺端)吸收活性的影响.
方法:取(80±5)g雄性SD大鼠6只,体外分离培养大鼠股骨组织的骨干和骨骺端,随机分为对照组、雌二醇组(estradiol,E)和蛇床子素处理组.同时在股骨组织分离培养48 h后采用终浓度为1×10-5 mol/L的蛇床子素和1×10-8 mol/L雌二醇对体外培养骨干和骨骺端进行处理;分别在药物处理后的第3、6、9、12 天后测定抗酒石酸酸性磷酸酶(Tartrate-resistant acid phosphatase,StrACP)活性、培养基中葡萄糖(Glucose,Glu)、乳酸(Lactic acid,La)的含量、Real-Time RT-PCR StrACP、集落刺激因子(Macrophage colony stimulating factor,MCSF)、组织激酶K(Cathepsin K,CTSK)mRNA的表达水平.
结果:1×10-5 mol/L蛇床子素组碱性磷酸酶(ALP)活性为2226,1×10-8 mol/L雌二醇组ALP活性为2498;与对照组比较1×10-5 mol/L蛇床子素和1×10-8 mol/L雌二醇组在加药处理后的第6、9和12天显着抑制StrACP酶活性(P<0.05);1×10-5 mol/L蛇床子素处理体外培养大鼠骨骺端和骨干组织的第3、6、9天后显着增加培养基中乳酸含量(P<0.05),并显着减少培养基中葡萄糖的含量(P<0.05).与对照组比较,1×10-5 mol/L的蛇床子素处理体外培养大鼠股骨组织后的第6和9天蛇床子素能显着抑制StrACP,MCSF,CTSK mRNA的表达水平(P<0.05).
结论:蛇床子素抑制体外培养大鼠股骨组织的骨骺端和骨干吸收活性同时可提高营养代谢水平. |
| [关键词]:蛇床子素 股骨 骨和骨组织 抗酒石酸酸性磷酸酶 |
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| Inhibition of osthole for resorption of rats femur tissue in vitro |
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Abstract:
Objective: To investigate osthole effect on femoral tissue resorption activity of rat in vitro.
Methods: Six SD rats weighted(80±5) g were used to isolate and culture femoral tissue (diaphyses and metaphysis) in vitro. The cultured tissue were devided into control group, estradiol group and osthole group. The femoral tissue was treated with final concentration of 1×10-5 mol/L osthole and 1×10-8 mol/L estradiol culture in vitro at 48 hours after cultured. Tartrate-resistant acid phosphatase (StrACP) activity, glucose and Lactic acid content, StrACP, MCSF(Macrophage colony stimulating factor) and CTSK (Cathepsin K) mRNA was detected by Real-Time RT-PCR were detected.
Results: Concetration of Alkaline phosphatase activity were 2226 and 2498 in 1×10-5 mol/L osthole and 1×10-8 mol/L estradiol respectively. As compared with control group, the activity of StrACP of 1×10-5 mol/L osthole and 1×10-8 mol/L estradiol were inhibited at 6, 9, 12 days(P<0.05); under treatment of in 1×10-5 mol/L osthole, the content of Lactic acid were increased and the content of glucose were decreased at 3, 6, 9 days(P<0.05); StrACP, MCSF and CTSK mRNA expression level were inhibited at 6, 9 days(P<0.05).
Conclusion: Osthole can inhibit bone resorption and raise the level of nutrition metabolism of femurs tissue. |
| KEYWORDS:Osthole Femur Bone and bones Tartrate-resistant acid phosphatase |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 周建,任雪梅,马小妮,高玉海,闫丽娟,石文贵,陈克明.蛇床子素对体外培养大鼠股骨组织吸收的抑制作用[J].中国骨伤,2015,28(9):832~837 |
| 英文格式: | ZHOU Jian,REN Xue-mei,MA Xiao-ni,GAO Yu-hai,YAN Li-juan,SHI Wen-gui,CHEN Ke-ming.Inhibition of osthole for resorption of rats femur tissue in vitro[J].zhongguo gu shang / China J Orthop Trauma ,2015,28(9):832~837 |
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