| 电针对大鼠急性脊髓损伤后神经细胞凋亡及相关功能的影响 |
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Received:December 24, 2014
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| 期刊信息：《中国骨伤》2015年28卷，第8期，第733-738页 |
| DOI：10.3969/j.issn.1003-0034.2015.08.013 |
| 基金项目:浙江省中医药科研计划项目(编号:2008CB067) |
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目的:探讨电针对大鼠急性脊髓损伤后膀胱功能改善的影响及作用机制。
方法:取健康成年雄性SPF级SD大鼠60只,体重220～250 g,适应性饲养1周后,将大鼠按照随机数字表法随机分为假手术组、模型组、电针组和电针对照组,各15只。假手术组不予任何刺激,模型组、电针组和电针对照组大鼠采用改良Allens法制作脊髓损伤中度损伤SD大鼠模型,模型组不予治疗,电针组给予秩边与水道穴电针治疗,电针对照组给予秩边与水道穴旁开0.5寸电针治疗,频率 2/100 Hz,电流 1 mA,刺激15 min,电针左右隔次交替,每日1次,共7次;分别于术后1、7 d观察大鼠残余尿量、排尿量的变化;术后7 d处死大鼠取伤段脊髓观察各组大鼠凋亡情况,检测Bcl-2、Bax、Bad含量的变化。
结果:造模后3组大鼠均出现不同程度的膀胱功能障碍。术后7 d,电针组、电针对照组残余尿量较术后1 d明显降低(P <0.001),且电针组与电针对照组比较差异有统计学意义(P <0.01);电针组、电针对照组较模型组在术后7 d排尿量增加,且电针组与电针对照组比较差异有统计学意义(P <0.001); TUNEL发现电针可以抑制脊髓神经细胞的凋亡,电针组、电针对照组与模型组相比在术后7 d脊髓神经细胞凋亡率显着增加(P <0.01,P <0.05),且电针组与电针对照组比较差异有统计学意义(P <0.05);与模型组相比,电针组、电针对照组Bax、Bad的阳性表达率降低(P <0.01,P <0.05),而Bcl-2的阳性表达率升高(P <0.01);且电针组与电针对照组比较差异有统计学意义(P <0.05).
结论:电针能明显促进急性脊髓损伤的修复,其机制可能为通过增加Bcl-2、抑制Bax、Bad的表达,从而抑制脊髓神经元细胞的凋亡发生作用的。 |
| [关键词]:电针 脊髓损伤 细胞凋亡 动物实验 |
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| Effects of electro-acupuncture on neuronal apoptosis and associative function in rats with spinal cord injury |
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Abstract:
Objective:To explore the effect of electro-acupuncture to improve the bladder function after acute spinal cord injury in rats and its possible mechanism.
Methods:Sixty healthy adult male SD rats of SPF grade,with body weight of 220 to 250 g,one week after feeding adaptation,were randomly divided into sham operation group,model group,electro-acupuncture group,electro-acupuncture control group with 15 rats in each group. Sham operation group underwent no stimulation,and the moderate damage model of spinal cord injury were made in other three groups according to modified Allens method. The model group were not treated,electro-acupuncture group were treated with electro-acupuncture on Zhibianxue and Shuidaoxue,and electro-acupuncture control group were treated with electro-acupuncture on 0.5 inch next to Zhibianxue and Shuidaoxue. The frequency of 2/100 Hz,current of 1 mA,stimulation time of 15 min,once a day,left and right alternately stimulate every time,for a total of 7 times. The changes of residual urine volume and urine output in rats at the 1st and the 7th days after operation were observed. And 7 d later,the rats were sacrificed and the injured spinal cord were taken out to observe the apoptosis,and to detect the changes of Bcl-2,Bax,Bad content.
Results:After modeling,the rats of three groups showed different bladder dysfunction. In electro-acupuncture group and electro-acupuncture control group,the residual urine volume of the 7th day after operation was significant lower than the 1st day after operation (P <0.001),and there was statistically significant difference on the 7th day after operation between two groups (P <0.001). Compared with model group,the urine output of electro-acupuncture group and electro-acupuncture control group was significantly increased on the 7th day after operation,and there was significant difference between electro-acupuncture group and electro-acupuncture control group (P <0.001). Electro-acupuncture can inhibit apoptosis of spinal cord neurons by TUNEL detection. Postoperative at 7 d,the rate of nerve cell apoptosis in electro-acupuncture group and electro-acupuncture control group was significant increased than model group(P <0.01,P <0.05),and there was significant difference between electro-acupuncture group and electro-acupuncture control group(P <0.005). Compared with model group,the positive expression rate of Bax,Bad decreased (P <0.01,P <0.05),and Bcl-2 increased(P <0.01) in electro-acupuncture group and electro-acupuncture control group,there was significant difference between electro-acupuncture group and electro-acupuncture control group(P <0.01).
Conclusion:Electro-acupuncture can obviously promote the repair of acute spinal cord injury,its mechanism may be through increasing Bcl-2,inhibiting the expression of Bax,Bad,which inhibits the apoptosis of spinal cord neurons. |
| KEYWORDS:Electric acupuncture Spinal cord injury Apoptosis Animal experimentation |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 李长明,谢尚举,王拓,杜伟斌,杨宗保,全仁夫.电针对大鼠急性脊髓损伤后神经细胞凋亡及相关功能的影响[J].中国骨伤,2015,28(8):733~738 |
| 英文格式: | LI Chang-ming,XIE Shang-ju,WANG Tuo,DU Wei-bin,YANG Zhong-bao,QUAN Ren-fu.Effects of electro-acupuncture on neuronal apoptosis and associative function in rats with spinal cord injury[J].zhongguo gu shang / China J Orthop Trauma ,2015,28(8):733~738 |
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