| 中药脊髓康对脊髓损伤大鼠Nogo-NgR基因表达的影响 |
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Received:July 25, 2014
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| 作者 | Author | 单位 | Unit | E-Mail |
| 尤武林 |
YOU Wu-lin |
南京中医药大学无锡附属医院骨科, 江苏无锡 214071 |
Department of Orthopaedics, Wuxi Affilliated Hospital of TCM of Nanjing University of Chinese Medicine, Wuxi 214071, Jiangsu, China |
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| 张亚峰 |
ZHANG Ya-feng |
南京中医药大学无锡附属医院骨科, 江苏无锡 214071 |
Department of Orthopaedics, Wuxi Affilliated Hospital of TCM of Nanjing University of Chinese Medicine, Wuxi 214071, Jiangsu, China |
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| 陈剑峰 |
CHEN Jian-feng |
南京中医药大学无锡附属医院骨科, 江苏无锡 214071 |
Department of Orthopaedics, Wuxi Affilliated Hospital of TCM of Nanjing University of Chinese Medicine, Wuxi 214071, Jiangsu, China |
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| 尹恒 |
YIN Heng |
南京中医药大学无锡附属医院骨科, 江苏无锡 214071 |
Department of Orthopaedics, Wuxi Affilliated Hospital of TCM of Nanjing University of Chinese Medicine, Wuxi 214071, Jiangsu, China |
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| 杨俊锋 |
YANG Jun-feng |
南京中医药大学无锡附属医院骨科, 江苏无锡 214071 |
Department of Orthopaedics, Wuxi Affilliated Hospital of TCM of Nanjing University of Chinese Medicine, Wuxi 214071, Jiangsu, China |
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| 王建伟 |
WANG Jian-wei |
南京中医药大学无锡附属医院骨科, 江苏无锡 214071 |
Department of Orthopaedics, Wuxi Affilliated Hospital of TCM of Nanjing University of Chinese Medicine, Wuxi 214071, Jiangsu, China |
osteowjw@163.com |
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| 期刊信息：《中国骨伤》2015年28卷，第3期，第235-239页 |
| DOI：10.3969/j.issn.1003-0034.2015.03.010 |
| 基金项目:江苏省自然科学基金资助项目(编号:BK2011180);江苏省2013年度普通高校研究生科研创新计划项目(编号:CXZZ13-0614) |
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目的:观察中药脊髓康对急性大鼠脊髓损伤后Nogo-A,NgR基因表达的影响,探讨其对急性脊髓损伤的治疗保护作用及机制。
方法:180只雌性SD大鼠,随机分为假手术组、模型组、强的松组及脊髓康高、中、低剂量组,每组30只。假手术组:切除T10节段椎板,不损伤脊髓;其余组采用改良Allen法建立脊髓损伤模型。强的松组:术后至处死前每日灌胃强的松0.06 g/kg,首次在术后30 min给药。脊髓康高、中、低剂量组:在同一时间分别给予脊髓康,每日50、25、12.5 g/kg.术后3、7、14 d分批处死动物,取损伤节段脊髓9例,分别利用Western Blot及Real-time PCR检测脊髓组织中Nogo-A、NgR蛋白及mRNA表达水平。
结果:假手术组在各时间点Nogo-A及NgR的表达维持在基础水平。与模型组比较,各时间点假手术组、强的松组及脊髓康中剂量组Nogo-A、NgR蛋白表达差异均有统计学意义,脊髓康高、低剂量组差异无统计学意义。术后3 d,各治疗组Nogo-A、NgR mRNA水平均低于模型组;术后7 d,模型组Nogo-A、NgR mRNA水平升至最高,14 d时有所下降,但仍高于其他治疗组,其中强的松、脊髓康中剂量组效果最显着,两者之间差异无统计学意义。
结论:在Allen急性大鼠脊髓损伤后,脊髓康能调节Nogo-A及NgR基因表达,激活Nogo-NgR信号通路,促进脊髓损伤后神经细胞再生。 |
| [关键词]:脊髓损伤 基因表达 中草药 活血通络 |
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| Effects of Jisuikang(脊髓康) on Nogo-NgR gene expression in spinal cord rats with injury |
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Abstract:
Objective:To study the effects of Jisuikang(脊髓康) on Nogo-NgR gene expression,and to explore the protective effects and mechanism of Jisuikang(脊髓康) on spinal cord injury in rats.
Methods:One hundred eighty female rats were randomly assigned to 6 groups(30 rats per group). Sham group:T10 lamina was resected only and spinal cord was untreated. Model group:spine cord injury(SCI) was created with a modified impinger of Allen's by impacting on the T10 spinal cord. Prednisolone group:Prednisolone (0.06 g/kg) was given by intragastric administration at a time interval of 24 hours after operation. The Jisuikang (脊髓康) high,moderate and low dose groups:Jisuikang (脊髓康) was supplied with different dose (50 g/kg,25 g/kg,12.5 g/kg) by intragastric administration in rats after operation,for the first time at 30 min after surgery. Animals were killed 3,7,14 days after surgery. The expression levels of Nogo-A and NgR were observed by Western Blot and Real-time PCR.
Results:The expression of Nogo-A and NgR was at the basic level at all time points in sham group. Compared with model group,the protein expression levels of Nogo-A and NgR in sham,prednisolone,Jisuikang(脊髓康) moderate dose groups were statistically significant at all time points(P<0.05). No difference was found in Jisuikang(脊髓康) high and low dose groups (P>0.05). Three days after surgery,the mRNA levels of Nogo-A and NgR in treatment group were significantly lower than that in model group(P<0.01);7 days after surgery,Nogo-A and NgR mRNA expression were dramatically upregulated and peaked;14 days after operation,the expression was decreased,but still significantly higher than that in other treatment groups(P<0.01). Prednisolone and Jisuikang (脊髓康) moderate dose groups showed the most significant effects among all groups,but there was no statistically significant difference between two groups(P>0.05).
Conclusion:The decoction Jisuikang (脊髓康) can promote the nerve cell regeneration by regulating Nogo-A and NgR gene expression,activating Nogo-NgR signaling pathways after acute spinal cord injury. |
| KEYWORDS:Spinal cord injuries Gene expression Drugs,Chinese herbal Activate blood dredging collaterals |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 尤武林,张亚峰,陈剑峰,尹恒,杨俊锋,王建伟.中药脊髓康对脊髓损伤大鼠Nogo-NgR基因表达的影响[J].中国骨伤,2015,28(3):235~239 |
| 英文格式: | YOU Wu-lin,ZHANG Ya-feng,CHEN Jian-feng,YIN Heng,YANG Jun-feng,WANG Jian-wei.Effects of Jisuikang(脊髓康) on Nogo-NgR gene expression in spinal cord rats with injury[J].zhongguo gu shang / China J Orthop Trauma ,2015,28(3):235~239 |
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