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大鼠椎骨错缝模型下丘脑及背根神经节P物质含量的实验研究
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作者Author单位UnitE-Mail
陈博 CHEN Bo 上海中医药大学附属曙光医院石氏伤科医学中心, 上海市中医药研究院骨伤科研究所, 上海 201203 Shi's Center of Orthopaedics and Traumatology, Shuguang Hospital Affiliated to Shanghai University of TCM, Institute of Orthopaedics and Traumatology, Shanghai Academy of TCM, Shanghai 201203, China  
林勋 LIN Xun 上海中医药大学附属曙光医院石氏伤科医学中心, 上海市中医药研究院骨伤科研究所, 上海 201203 Shi's Center of Orthopaedics and Traumatology, Shuguang Hospital Affiliated to Shanghai University of TCM, Institute of Orthopaedics and Traumatology, Shanghai Academy of TCM, Shanghai 201203, China  
庞坚 PANG Jian 上海中医药大学附属曙光医院石氏伤科医学中心, 上海市中医药研究院骨伤科研究所, 上海 201203 Shi's Center of Orthopaedics and Traumatology, Shuguang Hospital Affiliated to Shanghai University of TCM, Institute of Orthopaedics and Traumatology, Shanghai Academy of TCM, Shanghai 201203, China  
孔令军 KONG Ling-jun 上海中医药大学附属岳阳中西医结合医院推拿研究所, 上海 201203  
詹红生 ZHAN Hong-sheng 上海中医药大学附属曙光医院石氏伤科医学中心, 上海市中医药研究院骨伤科研究所, 上海 201203 Shi's Center of Orthopaedics and Traumatology, Shuguang Hospital Affiliated to Shanghai University of TCM, Institute of Orthopaedics and Traumatology, Shanghai Academy of TCM, Shanghai 201203, China shgsyjs@139.com 
程英武 CHENG Ying-wu 上海中医药大学附属岳阳中西医结合医院推拿研究所, 上海 201203  
石印玉 SHI Yin-yu 上海中医药大学附属曙光医院石氏伤科医学中心, 上海市中医药研究院骨伤科研究所, 上海 201203 Shi's Center of Orthopaedics and Traumatology, Shuguang Hospital Affiliated to Shanghai University of TCM, Institute of Orthopaedics and Traumatology, Shanghai Academy of TCM, Shanghai 201203, China  
期刊信息：《中国骨伤》2015年28卷，第1期，第75-77页
DOI：10.3969/j.issn.1003-0034.2015.01.019
基金项目:国家自然基金青年基金项目(编号:81202707);高等学校博士学科点专项科研基金(新教师类)(编号:20123107120006);上海高校青年教师培养资助计划(编号:ZZszy13024);海派中医流派传承研究基地(编号:ZYSNXD-CC-HPGC-JD-001);上海市中医药事业发展3年行动计划项目(编号:ZYSNXD-CC-ZDYJ047)


目的: 检测腰椎椎骨错缝对大鼠下丘脑及背根神经节中P物质含量的影响.

方法: 选取120只体质量为350～450 g的SPF级雄性SD大鼠随机分为旋转固定组(RF组)、单纯固定组(SF组)和假手术组(Sham组).RF组和SF组大鼠腰椎L4-L6节段植入的椎体外部连接固定装置,RF组大鼠使L5棘突向右侧旋转,造成L5棘突与L4和L6棘突的不共线;SF组大鼠单纯植入椎体外部连接固定装置不进行旋转.Sham组大鼠不植入椎体外部连接固定装置,只切开后缝合.分别于手术后1、4、8和12周结束时,检测各组大鼠下丘脑及背根神经节中P物质含量.

结果: 手术1、4、8周后,RF组和SF组大鼠下丘脑中P物质含量均低于Sham组大鼠 (P<0.05);手术1、4、8、12周后,RF组和SF组大鼠背根神经节中P物质含量均高于Sham组大鼠 (P<0.05);固定12周后,RF组和SF组大鼠下丘脑中P物质与Sham组比较差异无统计学意义(P >0.05).

结论: 椎骨错缝可抑制下丘脑中P物质的镇痛作用,促进背根神经节中P物质的合成和传递,从而引起或加剧疼痛.
[关键词]:骨错缝  下丘脑  背根神经节  P物质
 
Experimental research on substance P content of hypothalamus and dorsal root ganglia in rats with lumbar vertebrae gucuofeng model
Abstract:

Objective: To detect the effects of lumbar vertebrae Gucuofeng on the substance P content of hypothalamus and dorsal root ganglia in rat models.

Methods: A hundred and twenty SPF level SD male rats with the weight of 350 to 450 g were randomly divided into rotary fixation group (RF group),simple fixation group (SF group) and sham operation group (Sham group). The external link fixation system was implanted into the L4-L6 of rats in RF group and SF group;and in RF group,that the L5 spinous process was rotated to the right resulted in L4,L5,L6 spinous process not collinear;in SF group,the external link fixation system was simply implanted and not rotated. The rats of Sham group were not implanted the external link fixation system and only open and suture. The substance P content of hypothalamus and dorsal root ganglia were detected at 1,4,8,12 weeks after operation.

Results: Substance P content of hypothalamus in RF group and SF group was lower than Sham group at 1,4,8 weeks after operation (P<0.05). Substance P content of dorsal root ganglia was higher than Sham group at 1,4,8,12 weeks after operation (P<0.05). There was no significant differences in the substance P content of hypothalamus among three groups at 12 weeks after operation (P >0.05).

Conclusion: Lumbar vertebrae Gucuofeng can inhibit the analgesic activity of substance P in hypothalamus and promote the synthesis and transmission of substance P in dorsal root ganglia,so as to cause or aggravate the pain.
KEYWORDS:Gucuofeng  Hypothalamus  Dorsal root ganglia  Substance P
 
引用本文，请按以下格式著录参考文献：
中文格式:陈博,林勋,庞坚,孔令军,詹红生,程英武,石印玉.大鼠椎骨错缝模型下丘脑及背根神经节P物质含量的实验研究[J].中国骨伤,2015,28(1):75~77
英文格式:CHEN Bo,LIN Xun,PANG Jian,KONG Ling-jun,ZHAN Hong-sheng,CHENG Ying-wu,SHI Yin-yu.Experimental research on substance P content of hypothalamus and dorsal root ganglia in rats with lumbar vertebrae gucuofeng model[J].zhongguo gu shang / China J Orthop Trauma ,2015,28(1):75~77
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