| 地塞米松致骨质疏松肾阴虚型病证结合模型构建与评价的实验研究 |
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Received:July 06, 2013
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| 作者 | Author | 单位 | Unit | E-Mail |
| 夏炳江 |
XIA Bing-jiang |
浙江中医药大学, 浙江 杭州 310053 |
Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang, China |
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| 童培建 |
TONG Pei-jian |
浙江中医药大学, 浙江 杭州 310053 |
Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang, China |
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| 孙燕 |
SUN Yan |
浙江中医药大学, 浙江 杭州 310053 |
Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang, China |
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| 周罗瑜 |
ZHOU Luo-yu |
浙江中医药大学, 浙江 杭州 310053 |
Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang, China |
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| 金红婷 |
JIN Hong-ting |
浙江中医药大学, 浙江 杭州 310053 |
Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang, China |
xiabj2006@sina.com |
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| 期刊信息：《中国骨伤》2014年27卷，第8期，第673-679页 |
| DOI：10.3969/j.issn.1003-0034.2014.08.013 |
| 基金项目:国家自然科学基金项目（编号：81202710）；浙江省自然科学基金项目（编号：Y2111184）；浙江省中医药科技计划项目（编号：2009YA001）；浙江省科技厅“钱江人才”计划项目（编号：2012R10064） |
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目的：构建和评价地塞米松致骨质疏松肾阴虚型病证结合动物模型。
方法：雌性昆明小鼠45只，随机分为对照组、模型组、药物反证组（六味地黄丸），每组15只。模型组与六味地黄丸组采用肌肉注射地塞米松进 行造模，对照组予以等量生理盐水肌注，六味地黄丸组在进行激素造模的同时予以六味地黄丸灌胃。动态观察小鼠的症状、体征；6周后，测定血清ACTH、cAMP、cGMP、TSH、E2等来评价肾阴虚证；通过对骨密度、骨组织学等 来评估骨质疏松情况。
结果：①与对照组相比，模型组小鼠出现明显的毛发干枯、躁动、易激动等肾阴虚症状。②与对照组相比，模型组小鼠体重增加缓慢（P<0.01），肾上腺、脾脏、肝脏指数降低（P<0.01，P<0.01，P<0.01），ACTH和TSH含量升高（P<0.01，P<0.01），E2含量降低（P<0.01），cAMP/cGMP比值升高（P<0.05）.③模型组较对照组股骨近端、腰椎骨密度均明显下降（P<0.01，P<0.05），HE染色显示骨小梁排列稀疏、变细，数目减少，间距加宽，微骨折等。④与模型组比较，六味地黄丸组能部分拮抗HPA轴功能的紊乱，部分纠正cAMP/cGMP失衡，提高骨量。
结论：地塞米松肌注法构建肾阴虚骨质疏松病证结合模型是可行的，其造模因素单一，干扰因素少，可控性 相对较强，是值得推广的动物模型。 |
| [关键词]:肾阴虚 骨质疏松 地塞米松 动物模型 |
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| Methods and evaluations on the sterioid-induced osteoporosis mice model with the type of Kidney-Yin deficiency |
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Abstract:
Objective: To establish the steriod-induced osteoporosis model with the type of Kidney-Yin deficiency.
Methods: Totally 45 female Kunming mice were randomly divided into normal group,model group and Liuwei Dihuang pills(六味地黄丸)group. The model was established by intramuscular injecting of Dexamethasone. Liuwei Dihuang pills(六味地黄丸)group was administered orally with Liuwei Dihuang pills(六味地黄丸). The signs and symptoms of mice were observed dynamically. All the animals were sacrificed at the end of the 6th weeks. The level of ACTH,cAMP,cGMP,TSH and E2 in serum were detected to evaluate deficiency of Kidney-Yin. Morphological changes and bone density were observed to evaluate osteoporosis.
Results: ①Compared with control group,mice in model group appeared obvious Kidney-Yin deficiency symptoms,including hair dry,restlessness,excitability,hard stool,and yellow. ②Compared with control group,the weight of mice in model group gained slower (P<0.01); the index of adrenal gland,liver and spleen decreased (P<0.01,P<0.01,P<0.01);the level of ACTH and TSH increased (P<0.01,P<0.01),the level of E2 decreased (P<0.01) and the ratio of cAMP/cGMP increased (P<0.05). ③Compared with control group,the bone density of lumbar vertebra and femur in model group were significantly decreased (P<0.01,P<0.05); HE staining revealed osteoporosis in model group mice. ④However,the Liuwei Dihuang pills(六味地黄丸) group can partly antagonize the inhibition of the HPA axis,alter the disordered sex hormone and the ratio of cAMP/cGMP,and reverse the osteoporosis partly.
Conclusion: The model of osteoporosis with type of Kidney-Yin deficiency could be established by Dexamethasone intramuscular injection. With less interference,it wight be a stable and reliable modeling method for integration of disease and syndrome in TCM. |
| KEYWORDS:Kidney-Yin deficiency Osteoporosis Dexamethasone Animal model |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 夏炳江,童培建,孙燕,周罗瑜,金红婷.地塞米松致骨质疏松肾阴虚型病证结合模型构建与评价的实验研究[J].中国骨伤,2014,27(8):673~679 |
| 英文格式: | XIA Bing-jiang,TONG Pei-jian,SUN Yan,ZHOU Luo-yu,JIN Hong-ting.Methods and evaluations on the sterioid-induced osteoporosis mice model with the type of Kidney-Yin deficiency[J].zhongguo gu shang / China J Orthop Trauma ,2014,27(8):673~679 |
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