| 股密葆含药血清对高浓度地塞米松干预后成骨细胞增殖分化作用的影响 |
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Received:November 13, 2013
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| 作者 | Author | 单位 | Unit | E-Mail |
| 宋奕 |
SONG Yi |
上海中医药大学, 上海 201203 |
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| 姜宏 |
JINAG Hong |
苏州市中医医院, 江苏 苏州 215009 |
Suzhou Hospital of Traditional Chinese Medicine, Suzhou 215009, Jiangsu, China |
honghong751@126.com |
| 丁道芳 |
DING Dao-fang |
上海市中医药研究院骨伤科研究所, 上海 200021 |
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| 李玲慧 |
LI Ling-hui |
上海中医药大学, 上海 201203 |
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| 杜国庆 |
DU Guo-qing |
上海中医药大学, 上海 201203 |
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| 刘锦涛 |
LIU Jin-tao |
苏州市中医医院, 江苏 苏州 215009 |
Suzhou Hospital of Traditional Chinese Medicine, Suzhou 215009, Jiangsu, China |
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| 詹红生 |
ZHAN Hong-sheng |
上海市中医药研究院骨伤科研究所, 上海 200021 |
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| 期刊信息：《中国骨伤》2014年27卷，第8期，第668-672页 |
| DOI：10.3969/j.issn.1003-0034.2014.08.012 |
| 基金项目:国家自然科学基金资助项目（编号：81073114，81072830）；江苏省中医药管理局2010年度康缘中医药科技创新基金（编号：HZ1013KY）；苏州市科技计划项目（编号：SYS201257） |
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目的：观察股密葆含药血清对高浓度地塞米松干预后成骨细胞增殖分化的影响。
方法：用多次酶消化法分离出生24 h内新生SD大鼠颅盖骨成骨细胞，P1代细胞进行实验。将不同浓度地塞米松（0、10-8、10-7、 10-6、10-5、10-4 mol/L）干预成骨细胞，1周后进行碱性磷酸酶（alkaline phosphatase，ALP）染色，3周后进行矿化结节染色。根据上述实验结果，进一步选择10-5 mol/L 地塞米松干预成骨细胞1周，继而改换空白鼠血清 及高、中、低浓度股密葆含药血清培养基，培养1周后进行ALP染色，Western-blot检测Ⅰ型胶原及增殖细胞核抗原（proliferating cell nuclear antigen，PCNA）表达，培养2周后进行矿化结节染色。
结果：生理浓度地塞米 松（10-8 mol/L）可促进成骨细胞ALP的表达及矿化结节产生，而高浓度地塞米松（10-7~10-4 mol/L）可抑制上述结果，股密葆含药血清可逆转高浓度地塞米松对成骨细胞ALP、矿化结节、Ⅰ型胶原及PCNA的抑制作用。结论 ：高浓度地塞米松可抑制成骨细胞增殖分化，而这种抑制作用可被股密葆含药血清逆转。 |
| [关键词]:地塞米松 成骨细胞 细胞增殖 中草药 |
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| Effects of serum containing Gumibao（股密葆） on the proliferation and differentiation of osteoblast induced by dexamethasone |
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Abstract:
Objective: To investigate the effects of serum containing Gumibao(股密葆) on the proliferation and differentiation of osteoblast induced by dexamethasone.
Methods: Osteoblasts were extracted from skulls in newly born (within 24 hours) SD rats,and digested with collagenase. The first passage of cells were used for experiments. Cells were cultured in the medium containing different concentrations of dexamethasone(0、10-8、10-7、 10-6、10-5、10-4 mol/L). Alkaline phosphatase staining were carried out after 1 week and numbers of mineralized nodes with alizarin red staining were observed after 3 weeks. Accordingly,following the treatment of 10-5 mol/L dexamethasone for 1 week,cells were cultured in the medium with serum containing Gumibao(股密葆). One week after Cumibao(股密葆) treatment, cells were stained with Alkaline phosphatase and collagenⅠand PCNA were examined by Western-blot. However,the observation of numbers of mineralized nodes with alizarin red stain required one more week.
Results: High concentration of dexamethasone could inhibit the expression of PCNA,collagen I,alkaline phosphatase and reduce the number of mineralized nodes of osteoblast,while serum containing Gumibao(股密葆) could reverse the inhibition.
Conclusion: High concentration of dexamethasone could inhibit the proliferation and differentiation of osteoblastic cells,while serum containing Gumibao (股密葆) could reverse the inhibition. |
| KEYWORDS:Dexamethasone Osteoblasts Cell proliferation Drugs,Chinese herbal |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 宋奕,姜宏,丁道芳,李玲慧,杜国庆,刘锦涛,詹红生.股密葆含药血清对高浓度地塞米松干预后成骨细胞增殖分化作用的影响[J].中国骨伤,2014,27(8):668~672 |
| 英文格式: | SONG Yi,JINAG Hong,DING Dao-fang,LI Ling-hui,DU Guo-qing,LIU Jin-tao,ZHAN Hong-sheng.Effects of serum containing Gumibao（股密葆） on the proliferation and differentiation of osteoblast induced by dexamethasone[J].zhongguo gu shang / China J Orthop Trauma ,2014,27(8):668~672 |
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