| 河蚌提取物葡聚糖对软骨细胞Wnt通路的调控作用研究 |
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Received:November 10, 2013
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| 作者 | Author | 单位 | Unit | E-Mail |
| 韦宋谱 |
WEI Song-pu |
上海中医药大学附属曙光医院骨伤科, 上海 201203
台州恩泽医疗中心(集团)路桥医院中医骨伤科, 浙江 台州 318050 |
Department of Orthopaedics, Shuguang Hospital Affiliated to Shanghai University of TCM, Shanghai 201203, China |
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| 丁道芳 |
DING Dao-fang |
上海中医药大学附属曙光医院骨伤科, 上海 201203 |
Department of Orthopaedics, Shuguang Hospital Affiliated to Shanghai University of TCM, Shanghai 201203, China |
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| 王学宗 |
WANG Xue-zong |
上海中医药大学附属曙光医院骨伤科, 上海 201203 |
Department of Orthopaedics, Shuguang Hospital Affiliated to Shanghai University of TCM, Shanghai 201203, China |
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| 庞坚 |
PANG Jian |
上海中医药大学附属曙光医院骨伤科, 上海 201203 |
Department of Orthopaedics, Shuguang Hospital Affiliated to Shanghai University of TCM, Shanghai 201203, China |
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| 郑昱新 |
ZHENG Yu-xin |
上海中医药大学附属曙光医院骨伤科, 上海 201203 |
Department of Orthopaedics, Shuguang Hospital Affiliated to Shanghai University of TCM, Shanghai 201203, China |
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| 徐勤光 |
XU Qin-guang |
上海中医药大学附属曙光医院骨伤科, 上海 201203 |
Department of Orthopaedics, Shuguang Hospital Affiliated to Shanghai University of TCM, Shanghai 201203, China |
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| 曹月龙 |
CAO Yue-long |
上海中医药大学附属曙光医院骨伤科, 上海 201203 |
Department of Orthopaedics, Shuguang Hospital Affiliated to Shanghai University of TCM, Shanghai 201203, China |
ningtcm@126.com |
| 詹红生 |
ZHAN Hong-sheng |
上海中医药大学附属曙光医院骨伤科, 上海 201203 |
Department of Orthopaedics, Shuguang Hospital Affiliated to Shanghai University of TCM, Shanghai 201203, China |
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| 期刊信息：《中国骨伤》2014年27卷，第6期，第461-465页 |
| DOI：10.3969/j.issn.1003-0034.2014.06.005 |
| 基金项目:国家自然科学基金（编号：81072830）；上海市教委创新项目（编号：11YZ64）；上海中医药事业发展三年行动计划项目（编号：ZYSNXD-CC-YJXYY） |
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| 目的： 探讨河蚌肉提取物葡聚糖HBP-A（anodonta glucan HBP-A）对体外软骨细胞Wnt通路的调控作用。方法： 体外培养大鼠软骨细胞，添加IL-1β（10 ng/ml）诱导分化，分为空白组，IL-1β组，IL-1β+IWP-2（5 μM，Wnt通路抑制剂）组，IL-1β+ HBP-A（0.3 mg/ml）组，IL-1β+IWP-2 +HBP-A共5组培养，提取各组细胞RNA和蛋白，采用Rt-PCR检测各组细胞Wnt-3a、β-catenin（24、48、72 h）及MMP-13（72 h）的基因表达；采用Western-blot检测β-catenin、MMP-13、Sox-9和Coll-Ⅱ蛋白的表达水平（48 h）.结果： 细胞经IL-1β诱导分化，Wnt-3a基因表达升高，β-catenin以及MMP-13基因和蛋白表达升高，Sox-9和Coll-Ⅱ蛋白表达下调。添加HBP-A干预可以抑制 IL-1β诱导下软骨细胞Wnt-3a基因的高表达、β-catenin以及MMP-13基因和蛋白的高表达，上调Sox-9和Ⅱ型胶原蛋白的表达。IWP-2和HBP-A合用时，Wnt-3a、β-catenin基因以及β-catenin蛋白表达最低，Sox-9蛋白表达最高。结论： HBP-A可通过降低Wnt/β-catenin信号通路表达，延缓软骨细胞分化，并且可与Wnt抑制剂协同调节Wnt-3a、β-catenin和Sox-9因子的表达。 |
| [关键词]:组织提取物 软骨细胞 葡聚糖类 基因表达调控 |
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| Regulating effect of anodonta glucan HBP-A on chondrocytes through Wnt pathway |
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| Abstract:Objective: To investigate regulation function of anodonta glucan HBP-A on chondrocytes through Wnt pathway in vitro. Methods: Rat chondrocytes were cultured and differentiated induced with IL-1β(10 ng/ml) in vitro. Chondrocytes were divided into five groups:IL-1βgroup,IL-1β+IWP-2(5 μM,Wnt pathway inhibitor) group,IL-1β+ HBP-A (0.3 mg/ml) group and IL-1β+IWP-2 +HBP-A group. Wnt-3a,β-catenin (24 h,48 h,72 h) and MMP-13(72 h) genes expression were detected by Rt-PCR,while β-catenin,MMP-13,Sox-9 and coll-Ⅱ(48 h) protein expression were measured by Western-blot. Results: After induction of IL-1β,gene expression of Wnt-3a,β-catenin and MMP-13 were increased,so were the protein expression of β-catenin and MMP-13. In contrast,protein expression of Sox-9 and Coll-Ⅱ were declined. Following addition of HBP-A,Wnt-3a,β-catenin and MMP-13 were shown as induction of IL-1β,but protein expression of Sox-9 and Coll-Ⅱ were upgraded. Combining HBP-A with IWP-2 led to the lowest level in Wnt-3a,β-catenin gene and β-catenin protein expression and highest expression of Sox-9 protein. Conclusion: HBP-A could not only delay the differentiation of chondrocytes through downgrading the signal expression of Wnt/β-catenin,but also adjust the expression of Wnt-3a,β-catenin and Sox-9 when combinated with the Wnt inhibitor. |
| KEYWORDS:Tissue extracts Chondrocytes Glucans Gene expression regulation |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 韦宋谱,丁道芳,王学宗,庞坚,郑昱新,徐勤光,曹月龙,詹红生.河蚌提取物葡聚糖对软骨细胞Wnt通路的调控作用研究[J].中国骨伤,2014,27(6):461~465 |
| 英文格式: | WEI Song-pu,DING Dao-fang,WANG Xue-zong,PANG Jian,ZHENG Yu-xin,XU Qin-guang,CAO Yue-long,ZHAN Hong-sheng.Regulating effect of anodonta glucan HBP-A on chondrocytes through Wnt pathway[J].zhongguo gu shang / China J Orthop Trauma ,2014,27(6):461~465 |
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