| 聚乙二醇化骨形态发生蛋白-2基因转染兔骨髓间充质干细胞及其表达测定 |
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Received:June 03, 2013
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| 作者 | Author | 单位 | Unit | E-Mail |
| 丁立峰 |
DING Li-feng |
中国医科大学附属盛京医院创伤骨科, 辽宁 沈阳 110004 |
Department of Trauma, Shengjing Hospital of Chinese Medical University, Shenyang 110004, Liaoning, China |
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| 郑刚 |
ZHENG Gang |
中国医科大学附属盛京医院创伤骨科, 辽宁 沈阳 110004 |
Department of Trauma, Shengjing Hospital of Chinese Medical University, Shenyang 110004, Liaoning, China |
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| 杨军 |
YANG Jun |
中国医科大学附属盛京医院创伤骨科, 辽宁 沈阳 110004 |
Department of Trauma, Shengjing Hospital of Chinese Medical University, Shenyang 110004, Liaoning, China |
yangj1@sj-hospital.org |
| 周振东 |
ZHOU Zhen-dong |
中国医科大学附属盛京医院创伤骨科, 辽宁 沈阳 110004 |
Department of Trauma, Shengjing Hospital of Chinese Medical University, Shenyang 110004, Liaoning, China |
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| 李建军 |
LI Jian-jun |
中国医科大学附属盛京医院创伤骨科, 辽宁 沈阳 110004 |
Department of Trauma, Shengjing Hospital of Chinese Medical University, Shenyang 110004, Liaoning, China |
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| 期刊信息：《中国骨伤》2014年27卷，第1期，第48-53页 |
| DOI：10.3969/j.issn.1003-0034.2014.01.013 |
| 基金项目:辽宁省自然科学基金资助项目（编号：20082118）；辽宁省教育厅科学技术研究资助项目（编号：L2010652） |
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目的：聚乙二醇化骨形态发生蛋白-2基因（PEG/BMP-2）纳米颗粒转染兔骨髓间充质干细胞（rBMSCs），检测骨形态发生蛋白-2（BMP-2）在靶细胞中的表达。
方法：原代分离培养rBMSCs，分别采用PEG/BMP-2、脂质体/BMP-2转染细胞，采用流式细胞仪检测转染效率，采用Western Blot和real time RT-PCR方法检测BMP-2表达。
结果：成功制备出PEG/BMP-2纳米颗粒并将PEG/BMP-2转染至rBMSCs，转染细胞中BMP-2呈高表达，且与脂质体转染法相比，具有更高的转染效率。
结论：PEG/BMP-2纳米颗粒转染rBMSCs可高表达BMP-2，为骨缺损治疗修复提供新的治疗方法。 |
| [关键词]:间充质干细胞 骨形态发生蛋白质类 纳米颗粒 转染 兔 |
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| Study on bone mesenchymal stem cells transfected by polyethylene glycol/bone morphogenetic protein-2 |
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Abstract:
Objective: Polyethylene glycol/bone morphogenetic protein-2（PEG/BMP-2） nanoparticles were transfected into Rabbit bone mesenchymal stem cells（rBMSCs） and the expression of BMP-2 was detected.
Methods: Dissociated rBMSCs were primarily cultured in vitro and BMP-2 gene was transfected into rBMSCs by PEG/BMP-2 nanoparticals and lipofectamine,respectively. The efficiency of transfection was detected by flow cytometry and the expression of BMP-2 was detected by Western Blot and real time RT-PCR.
Results: PEG/BMP-2 nanoparticals were successfully synthesized and transfected into rBMSCs. Compared with the lipofectamine transfection group,PEG/BMP-2 transfection group had higher efficiency and higher BMP-2 expression.
Conclusion: PEG/BMP-2 nanoparticals transfected rBMSCs highly expressed BMP-2,which provided novel strategies for the treatment of bone defect. |
| KEYWORDS:Mesenchymal stem cells Bone morphogenetic proteins Nanoparticals Transfection Rabbits |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 丁立峰,郑刚,杨军,周振东,李建军.聚乙二醇化骨形态发生蛋白-2基因转染兔骨髓间充质干细胞及其表达测定[J].中国骨伤,2014,27(1):48~53 |
| 英文格式: | DING Li-feng,ZHENG Gang,YANG Jun,ZHOU Zhen-dong,LI Jian-jun.Study on bone mesenchymal stem cells transfected by polyethylene glycol/bone morphogenetic protein-2[J].zhongguo gu shang / China J Orthop Trauma ,2014,27(1):48~53 |
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