| 重组人骨保护素与重组核因子κb活化因子受体蛋白对破骨前体细胞分化的影响 |
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Received:June 20, 2012
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| 作者 | Author | 单位 | Unit | E-Mail |
| 熊琦 |
XIONG Qi |
中国人民解放军总医院骨科, 北京 100853 |
Department of Orthopaedics, General Hospital of Chinese PLA, Beijing 100853, China |
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| 张里程 |
ZHANG Li-cheng |
中国人民解放军总医院骨科, 北京 100853 |
Department of Orthopaedics, General Hospital of Chinese PLA, Beijing 100853, China |
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| 张立海 |
ZHANG Li-hai |
中国人民解放军总医院骨科, 北京 100853 |
Department of Orthopaedics, General Hospital of Chinese PLA, Beijing 100853, China |
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| 姚琦 |
YAO Qi |
中国人民解放军总医院骨科, 北京 100853 |
Department of Orthopaedics, General Hospital of Chinese PLA, Beijing 100853, China |
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| 唐佩福 |
TANG Peifu |
中国人民解放军总医院骨科, 北京 100853 |
Department of Orthopaedics, General Hospital of Chinese PLA, Beijing 100853, China |
pftang301@126.com |
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| 期刊信息：《中国骨伤》2013年26卷，第4期，第324-327页 |
| DOI：10.3969/j.issn.1003-0034.2013.04.015 |
| 基金项目:国家自然科学基金(编号:81000796;30973068) |
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目的: 对比重组人骨保护素(rhOPG-Fc)与重组核因子κb活化因子受体蛋白(rhRANK)对破骨前体细胞分化的影响。
方法: 采用成骨细胞与破骨前体细胞RAW264.7混合培养, 在地塞米松、1,25(OH)2VitD3诱导下生成破骨细胞的方法。研究分3组:rhRANK组:10-5 g/L;rhOPG-Fc组:10-5 g/L;空白对照组。作用9 d后观察破骨细胞数目和形态, 抗酒石酸酸性磷酸酶(TRAP)染色阳性细胞个数, 骨磨片吸收陷窝计数。
结果: 在空白对照组, 小鼠成骨细胞与破骨前体细胞RAW264.7混合培养6 d后, 开始出现多核细胞, 9 d时可见大量成熟多核细胞, 经TRAP染色证实为成熟破骨细胞, 而rhRANK组及rhOPG-Fc 组TRAP染色阳性多核细胞数较对照组均减少, 特别是rhRANK组减少更明显。骨片吸收陷窝计数显示rhRANK组及rhOPG-Fc 组较对照组也明显减少, 而相对来说, rhRANK组较rhOPG-Fc组更少。
结论: rhOPG-Fc与rhRANK均可以有效抑制破骨前体细胞分化成为成熟破骨细胞, 且rhRANK较rhOPG-Fc抑制效果更明显。 |
| [关键词]:骨保护素 核因子κb活化因子受体蛋白 破骨前体细胞 细胞分化 |
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| Effects of recombinant human osteoprotegerin and recombinant RANK protein on the differentiation of osteoclast precursors |
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Abstract:
Objective: To compare the effect of recombinant OPG-Fc and recombinant RANK protein on the differentiation of osteoclast precursors.
Methods: Mouse osteoblasts cell lines were incubated with osteoclast precursors cell lines RAW 264.7 for 9 days with 10-5 g/L rhRANK or rhOPG-Fc or PBS added to the coculture system. TRAP stain positive cells counting and cortical bone pit formation counting were performed in the 9th day.
Results: Multinuleated TRAP stain positive cells were observed in the cocluture systems after 6 days incubation,and plenty of mature osteoclasts could be observed in the 9th day. With the addition of 10-5 g/L rhOPG-Fc or rhRANK,multinucleated giant cells and cortical bone pit formation couting decreased significantly compared with the control group,and the rhRANK group decreased more significantly than the rhOPG-Fc group.
Conclusions: Both rhOPG-Fc and rhRANK can inhabit the differentiation of osteoclast precursors and prevent them forming mature osteoclasts,moreover,the rhRANK shows the significant inhabition effect than the rhOPG-Fc. |
| KEYWORDS:Osteoprotegerin (OPG) Receptor activator of nuclear factor κb protein (RANK) Osteoclast precursor Cell differantiation |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 熊琦,张里程,张立海,姚琦,唐佩福.重组人骨保护素与重组核因子κb活化因子受体蛋白对破骨前体细胞分化的影响[J].中国骨伤,2013,26(4):324~327 |
| 英文格式: | XIONG Qi,ZHANG Li-cheng,ZHANG Li-hai,YAO Qi,TANG Peifu.Effects of recombinant human osteoprotegerin and recombinant RANK protein on the differentiation of osteoclast precursors[J].zhongguo gu shang / China J Orthop Trauma ,2013,26(4):324~327 |
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