| 金刚健骨片调节整合素β1和αⅤβ3表达水平的实验研究 |
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Received:June 25, 2012
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| 作者 | Author | 单位 | Unit | E-Mail |
| 杨少锋 |
YANG Shao-feng |
湖南中医药大学附属第一医院,湖南 长沙 410007 |
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| 李玲慧 |
LI Ling-hui |
上海中医药大学附属曙光医院石氏伤科医学中心,上海 201203 |
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| 陈青 |
CHEN Qing |
湖南中医药大学附属第一医院,湖南 长沙 410007 |
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| 姚共和 |
YAO Gong-he |
湖南中医药大学附属第一医院,湖南 长沙 410007 |
The First Hospital Affiliated to Hunan University of Traditional Chinese Medicine,Changsha 410007,Hunan,China |
proygh@163.com |
| 邓博 |
DENG Bo |
湖南中医药大学附属第一医院,湖南 长沙 410007 |
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| 向剑锋 |
XIANG Jian-feng |
湖南中医药大学附属第一医院,湖南 长沙 410007 |
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| 聂颖 |
NIE Ying |
湖南中医药大学附属第一医院,湖南 长沙 410007 |
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| 罗振华 |
LUO Zhen-hua |
湖南中医药大学附属第一医院,湖南 长沙 410007 |
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| 郭彦涛 |
GUO Yan-tao |
湖南中医药大学附属第一医院,湖南 长沙 410007 |
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| 期刊信息：《中国骨伤》2013年26卷，第2期，第138-141页 |
| DOI：10.3969/j.issn.1003-0034.2013.02.014 |
| 基金项目:国家教育部新教师基金项目资助 (编号：20094323120005) |
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目的：通过对去势大鼠整合素表达水平的观察,揭示金刚健骨片治疗骨质疏松症的作用机制.
方法：将50只10月龄Wistar雌性大鼠随机分为5组：福善美组(FSM)、金刚健骨片组(JGJG)、骨松宝颗粒组(GSB)、模型组(OVX)、假手术组(SHAM).治疗组及模型组行卵巢摘除术,假手术组切除相同重量的肠系膜.统一饲养13周造模成功后,治疗组分别以相应药物连续给药13周,JGJG组每只大鼠每天给药0.13 g,GSB组每只每天给药0.86 g,FSM组每只每天给药0.28 mg,模型组及假手术组以生理盐水替代.治疗结束后处死动物取出骨组织,进行骨密度测定,并利用免疫组化法测定整合素αⅤβ3及β1表达水平.
结果：骨密度测定结果显示,治疗组的股骨密度、整合素β1均较OVX组有明显提高;治疗组整合素αⅤβ3明显低于OVX组.
结论：金刚健骨片能显着提高去势大鼠的股骨密度、整合素β1的表达水平,并可降低整合素αⅤβ3的阳性表达率,从而干预成骨-破骨细胞偶联,达到治疗目的. |
| [关键词]:骨质疏松 整合素类 卵巢切除术 骨密度 |
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| Effect of Jingang Jiangu pill(金刚健片) on expression of integrin β1 and αⅤβ3 in ovariectomized osteoporosis model rats |
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Abstract:
Objective:To investigate the regulatory effect of Jingang Jiangu pill(金刚健片,JGJG) on expression of integrin in ovariectomized rats.
Methods:Fifty ovariectomized 10 months old female rats were randomly divided into 5 groups:Fushanmei group (FSM),Jingang Jiangu pill(金刚健片)group(JGJG),Gusongbao granule group(GSB),Model group(OVX),Sham group. After ovariectomized,the rats were raised in the same environment for 13 weeks. The rats in JGJG group took 0.13 g JGJG pill orally each day for each rat;the rats in GSB group took 0.86 g GSB granule orally each day for each rat;the rats in FSM group took 0.28 mg FSM orally each day for each rat;and the rats in OVX and sham groups took sodium. The treatment duration of rats in above 5 groups was 13 weeks. Bone mineral density(BMD) and the expression of integrin β1 and αⅤβ3 were detected in each group after the treatment.
Results:The BMD and the expression of integrin β1 in FSM group,JGJG group and GSB group improved obviously than that of OVX group. There were statistical difference between these groups(P < 0.05). The expression of integrin αvβ3 of the three treating groups significantly depressed.
Conclusion:The JGJG pill improves BMD and express of integrin β1 in ovariectomized rats and reduces express of integrin αⅤβ3 through the regulation of the coupling of osteoblasts and osteoclasts. |
| KEYWORDS:Osteoporosis Integrins Ovariectoy Bone density |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 杨少锋,李玲慧,陈青,姚共和,邓博,向剑锋,聂颖,罗振华,郭彦涛.金刚健骨片调节整合素β1和αⅤβ3表达水平的实验研究[J].中国骨伤,2013,26(2):138~141 |
| 英文格式: | YANG Shao-feng,LI Ling-hui,CHEN Qing,YAO Gong-he,DENG Bo,XIANG Jian-feng,NIE Ying,LUO Zhen-hua,GUO Yan-tao.Effect of Jingang Jiangu pill(金刚健片) on expression of integrin β1 and αⅤβ3 in ovariectomized osteoporosis model rats[J].zhongguo gu shang / China J Orthop Trauma ,2013,26(2):138~141 |
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