| 右归饮防治人工关节假体周围骨溶解的实验研究 |
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Received:May 25, 2012
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| 作者 | Author | 单位 | Unit | E-Mail |
| 蔡运火 |
CAI Yun-huo |
浙江中医药大学,浙江 杭州 310053 |
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| 赵鹏 |
ZHAO Peng |
浙江中医药大学,浙江 杭州 310053 |
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| 毛强 |
MAO Qiang |
浙江中医药大学,浙江 杭州 310053 |
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| 鲍航行 |
BAO Hang-xing |
浙江中医药大学,浙江 杭州 310053 |
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| 王金法 |
WANG Jin-fa |
浙江中医药大学,浙江 杭州 310053 |
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| 胡淼锋 |
HU Miao-feng |
浙江中医药大学,浙江 杭州 310053 |
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| 肖鲁伟 |
XIAO Lu-wei |
浙江中医药大学,浙江 杭州 310053 |
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| 童培建 |
TONG Pei-jian |
浙江省中医院,浙江 杭州 310006 |
Zhejiang Province Chinese Medicine Hospital, Hangzhou 310006, Zhejiang, China |
tongpeijian@163.com |
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| 期刊信息：《中国骨伤》2012年25卷，第12期，第1010-1015页 |
| DOI：10.3969/j.issn.1003-0034.2012.12.010 |
| 基金项目:国家自然科学基金(编号:30873276) |
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目的：观察右归饮和鲑鱼降钙素防治人工关节假体周围骨溶解的效果。
方法：将8周龄大的32只SD雄性大鼠,体重(250±20) g,随机分为4组:空白对照组、模型组、鲑鱼降钙素组和右归饮组,每组8只。空白对照组不做处理,另24只大鼠水合氯醛麻醉后,经髌旁内侧入路行双膝关节手术暴露,从股骨髁间窝钻孔至股骨髓腔,将包含高密度聚乙烯颗粒的凝胶注入孔中,然后放入钛钉,用骨蜡封闭窗口后逐层缝合。造模完成后,空白对照组和模型对照组均用生理盐水灌胃,右归饮组灌服右归饮汤剂,降钙素组则采用臀大肌注射鲑鱼降钙素。给药10周后处死大鼠,取大鼠动脉血和双侧股骨组织进行血生化、影像形态学、组织病理学和分子基因生物学检测。
结果：右归饮组和降钙素组破骨细胞激活的关键基因表达均受抑制。右归饮组OPG、Ca、ALP血清水平显着高于降钙素组(P<0.01);右归饮组血清RANKL含量显着低于降钙素组(P<0.01);两组RANK、Trap5b、P水平差异无统计学意义。
结论：右归饮和降钙素均有延缓和治疗人工关节假体周围骨吸收的功效,且右归饮比鲑鱼降钙素更能促进成骨形成。右归饮促进成骨,抑制破骨的功效为人工关节假体周围骨溶解提供了一个新的治疗手段。 |
| [关键词]:关节 假体 降钙素 大鼠 |
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| Experimental study on Yougui recipe(右归饮)in preventng osteolysis surrounding artificial prosthesis |
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Abstract:
Objective:To explore effects of Yougui recipe(右归饮) and salmon calcitonin acetate in preventing osteolysis surrounding artificial prosthesis.
Methods:Thirty-two SD male rats with weighted(250±20) g,aged 8 weeks,were randomly divided into four groups:blank group,model group,salmon calcitonin acetate group and Yougui recipe(右归饮) group,and 8 rats in each group. Blank group did not undergo any process,other 24 rats underwent anesthesia by chloral hydrate,their knee joints were exposed through medial patellar side,drilling from fermoral condyle nest to marrow cavity,high density of polythlene particles were injected into hole,titanium nail were put into,bone wax closed the window,then suturing step by step. After the molding,saline were used to gavaged in blank group and model group,Yougui recipe(右归饮) for Yougui recipe(右归饮) group,salmon calcitonin maximus injection for calcitonin group. After 10 weeks' mediation,rats were executed,and arterial blood and bilateral femoral organization were collected to biochemical,imaging morphology,tissue pathology and molecular biology detection.
Results:The key gene expression of activiting osteoclast were inhibited in Yougui recipe(右归饮) group and calcitonin group. The level of OPG,Ca,ALP in Yougui recipe(右归饮) group were higher than calcitonin group(P<0.01); the content of RANKL were lower(P<0.01). There were no significance meaning in RANK,Trap5b,P between two groups.
Conclusion:Both of Yougui recipe(右归饮) and calcitonin can slow and treat surrounding osteolysis of artificial joint prosthesis,and Yougui recipe(右归饮) has better effect in promoting bone formation. The effect of Yougui recipe(右归饮) in promoting bone formation,inhibiting osteoclasts to provide a new method to treating surrounding osteolysis of artificial joint prosthesis. |
| KEYWORDS:Joints Prostheses and implants Calcitoin Rats |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 蔡运火,赵鹏,毛强,鲍航行,王金法,胡淼锋,肖鲁伟,童培建.右归饮防治人工关节假体周围骨溶解的实验研究[J].中国骨伤,2012,25(12):1010~1015 |
| 英文格式: | CAI Yun-huo,ZHAO Peng,MAO Qiang,BAO Hang-xing,WANG Jin-fa,HU Miao-feng,XIAO Lu-wei,TONG Pei-jian.Experimental study on Yougui recipe(右归饮)in preventng osteolysis surrounding artificial prosthesis[J].zhongguo gu shang / China J Orthop Trauma ,2012,25(12):1010~1015 |
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