| 补肾强骨方含药血清对滑膜成纤维细胞增殖及PCNA和Bcl-2表达的影响 |
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Received:May 04, 2012
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| 期刊信息：《中国骨伤》2012年25卷，第11期，第942-945页 |
| DOI：10.3969/j.issn.1003-0034.2012.11.016 |
| 基金项目:上海市科委资助项目(编号：09411967400);上海市长宁区青年课题项目(编号：20094Q03001) |
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目的：探讨补肾强骨方是否可以抑制类风湿关节炎患者滑膜成纤维细胞增殖及PCNA mRNA、Bcl-2 mRNA表达.
方法：制作补肾强骨方含药血清,体外分离培养类风湿关节炎患者的滑膜细胞,并鉴定、传代.实验分组：含20%空白血清组、20%补肾强骨方高剂量含药血清组、20%补肾强骨方低剂量含药血清组、20%雷公藤多苷片含药血清组.以雷公藤多苷片含药血清组含药血清作为阳性对照,将20%浓度的含药血清加入培养传代的第3代滑膜细胞,继续培养.观察药物对滑膜细胞增殖及PCNA mRNA、Bcl-2 mRNA表达的影响.
结果：与空白对照组相比,补肾强骨方含药血清具有抑制滑膜成纤维细胞增殖的作用(P<0.000 1),且高剂量更显着.与空白对照血清相比,补肾强骨方含药血清均能显着抑制滑膜成纤维细胞PCNA mRNA、Bcl-2 mRNA表达(P<0.000 1).
结论：补肾强骨方含药血清能够显着抑制滑膜成纤维细胞增殖,可能是通过调节PCNA mRNA、Bcl-2 mRNA表达来实现的. |
| [关键词]:滑膜 成纤维细胞 中草药 补肾 |
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| Effect of Bushen Qianggu decoction(补肾强骨方) on the proliferation of synovial fibroblasts and expression of PCNA and Bcl-2 |
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Abstract:
Objective:To observe the effects of Bushen Qianggu decoction(补肾强骨方,BQD) on synovial fibroblasts proliferation and PCNA and Bcl-2 expression.
Methods:Serum containing BQD was made and synovial fibroblasts were separated and cultured and passaged in vitro. Four groups were divided as 20% blank control group,serum containing 20% Tripterygium wilfordii multi-glycosides drug(TWMD),20% of serum containing high and low of BQD,respectively. Serum containing drugs of different concentration were added into the synovial fibroblasts of the third generation,and then the synovial fibroblasts were cultured continued. The effects of different drugs on synovial fibroblasts and PCNA and Bcl-2 expression were observed.
Results:Compared with the control serum,BQD-containing serum promoted the apoptosis of synovial fibroblasts(P<0.000 1); especially,high dose could inhibit proliferation. The expression of PCNA and Bcl-2 was significantly lower in BQD-containing serum (P<0.000 1 vs control group).
Conclusion:BQD can promote the apoptosis of synovial fibroblasts by improving of expression of PCNA and Bcl-2,which may be one of the mechanisms of BQD in preventing and treating osteoporosis of rheumatoid arthritis. |
| KEYWORDS:Synovial membrane Fibroblasts Drugs,Chinese herbal Reinforcing kidney |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 高华利,欧阳桂林,黄新星,李宁丽,肖涟波.补肾强骨方含药血清对滑膜成纤维细胞增殖及PCNA和Bcl-2表达的影响[J].中国骨伤,2012,25(11):942~945 |
| 英文格式: | GAO Hua-li,OUYANG Gui-lin,HUANG Xin-xing,LI Ning-li,XIAO Lian-bo.Effect of Bushen Qianggu decoction(补肾强骨方) on the proliferation of synovial fibroblasts and expression of PCNA and Bcl-2[J].zhongguo gu shang / China J Orthop Trauma ,2012,25(11):942~945 |
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