| 膝关节骨关节炎发病过程中HSP70对软骨细胞凋亡的影响 |
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Received:February 21, 2012
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| 作者 | Author | 单位 | Unit | E-Mail |
| 徐守宇 |
XU Shou-yu |
浙江中医药大学附属第三医院康复科,浙江 杭州 310005
日本顺天堂大学医学部,日本 东京 113-8421 |
Department of Rehabilitation,the 3rd Hospital Affiliated to Zhejiang University of Traditional Chinese Medicine,Hangzhou 310005,Zhejiang,China |
overnightjo@msn.com |
| 姚新苗 |
YAO Xin-miao |
浙江中医药大学附属第三医院康复科,浙江 杭州 310005 |
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| 翟云 |
ZHAI Yun |
浙江中医药大学附属第三医院康复科,浙江 杭州 310005 |
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| 潘文胜 |
PAN Wen-sheng |
浙江大学医学院附属第二医院病理科,浙江 杭州 310009 |
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| 方针 |
FANG Zhen |
浙江中医药大学附属第三医院康复科,浙江 杭州 310005 |
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| 何帮剑 |
HE Bang-jian |
浙江中医药大学附属第三医院康复科,浙江 杭州 310005 |
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| 许晶虹 |
XU Jing-hong |
浙江大学医学院附属第二医院病理科,浙江 杭州 310009 |
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| 期刊信息：《中国骨伤》2012年25卷，第10期，第846-851页 |
| DOI：10.3969/j.issn.1003-0034.2012.10.015 |
| 基金项目:1.浙江省教育厅高校科研计划基金项目(编号:Y200907379);2.浙江省“重中之重”学科建设经费资助课题(浙教高科[2008]255号) |
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目的：观察热休克蛋白70(HSP70)和半胱氨酸天冬氨酸蛋白酶-3蛋白 (caspase-3)在膝关节骨关节炎(knee osteoarthritis,KOA)发病过程中的表达,探讨其相关性.
方法：40只3月龄SD大鼠分为两组,实验组30只,对照组10只.实验组采用Hulth 法复制膝骨性关节炎模型,作前交叉韧带切断加内侧1/3半月板切除术,对照组不作特殊处理.造模后,不采取任何措施,自由饲养.分别在术后1、2、4周取材股骨端和胫骨端关节,对标本组织进行肉眼、免疫组化及光镜观察,采用Mankin改良的关节软骨病理评分标准进行组织学评分.
结果：肉眼及光镜观察,实验组均可见KOA的改变,如滑膜增生、表层软骨糜烂等;同时,免疫组化中,术后1、2、4周HSP70的表达逐渐增加,caspase-3的表达先增加后降低,而对照组并无类似改变.Mankin评分显示1周分别和2、4周比较,差异均有统计学意义(P < 0.01).
结论：膝关节骨关节炎发病过程中,热休克蛋白抑制软骨细胞凋亡,并对软骨细胞具有保护作用,这将为临床上诸保守治疗提供一定程度的客观科学依据. |
| [关键词]:骨关节炎,膝 热休克蛋白70 半胱氨酸天冬氨酸蛋白酶-3 软骨细胞 细胞凋亡 |
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| Effect of HSP70 on apoptotic of cartilage cells in knee osteoarthritis |
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Abstract:
Objective:To investigate the expression and relationship between HSP70 and caspase-3 in knee osteoarthritis.
Methods:Forty adult SD rats were divided into experimental group and control group. Thirty rats in experimental group,anterior cruciate ligament transaction (ACLT) was cut off and partial meniscectomy of 1/3 inside incision were performed to reproduce knee osteoarthritis (KOA) model according to Hulth methos,and the other 10 rats was treated with nothing as control group. The rats were sent to the cage and free to move. At 1,2 and 4 weeks later,the arthritis cartilage of femoral and tibial end were observed through immunohistochemistry staining and light microscope. Meanwhile,Mankin scale system was adopted for histomorphology evaluation.
Results:Changes of KOA such as hyperplastic synovium,erosion on the surface of cartilage and so on were found in experiment group,the expression of HSP70 was augmentation all the time,but the expression of caspase-3 was reduction 1 week later; no similar changes were found in control group. Mankin scale system showed that there were significant differences in the first week as compared with the second week and 4th week(both P < 0.01).
Conclusion: Heat shock protein inhibit the apoptosis of cartilage cells and protect the cartilage cells in knee osteoarthritis,the conservative treatment for clinical provide objective scientific basis. |
| KEYWORDS:Osteoarthritis, knee Heat-shock proteins 70 Caspase-3 Chrondrocytes Apoptosis |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 徐守宇,姚新苗,翟云,潘文胜,方针,何帮剑,许晶虹.膝关节骨关节炎发病过程中HSP70对软骨细胞凋亡的影响[J].中国骨伤,2012,25(10):846~851 |
| 英文格式: | XU Shou-yu,YAO Xin-miao,ZHAI Yun,PAN Wen-sheng,FANG Zhen,HE Bang-jian,XU Jing-hong.Effect of HSP70 on apoptotic of cartilage cells in knee osteoarthritis[J].zhongguo gu shang / China J Orthop Trauma ,2012,25(10):846~851 |
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