| 适宜电刺激对成骨细胞平面培养下细胞增殖分化及BMP-2表达的实验研究 |
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Received:January 13, 2012
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| 作者 | Author | 单位 | Unit | E-Mail |
| 王伟 |
WANG Wei |
温州医学院第二临床学院,浙江 温州 325027 |
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| 李秀翠 |
LI Xiu-cui |
温州医学院第二临床学院,浙江 温州 325027 |
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| 程少文 |
CHENG Shao-wen |
温州医学院第二临床学院,浙江 温州 325027 |
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| 陈庆玉 |
CHEN Qing-yu |
温州医学院第二临床学院,浙江 温州 325027 |
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| 彭磊 |
PENG Lei |
海南医学院附属医院急诊创伤中心,海南 海口 570102 |
Department of Orthopaedics,Hospital Affiliated to Medical College of Hannan,Haikou 570102,Hannan,China |
xiaobo197518@163.com |
| 林忠勤 |
LIN Zhong-qin |
温州医学院第二临床学院,浙江 温州 325027 |
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| 张伟 |
ZHANG Wei |
温州医学院第二临床学院,浙江 温州 325027 |
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| 寇冬权 |
KOU Dong-quan |
温州医学院第二临床学院,浙江 温州 325027 |
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| 期刊信息：《中国骨伤》2012年25卷，第7期，第587-591页 |
| DOI：10.3969/j.issn.1003-0034.2012.07.014 |
| 基金项目:浙江省科技厅大学生孵化项目研究内容(编号：2010R413060) |
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目的：观察适宜电流刺激成骨细胞在平面培养下成骨细胞增殖及分化,明确是否具有促进增殖、分化作用,并观察电流刺激下BMP-2表达的变化。
方法： 将来自家兔幼仔颅骨提取的成骨细胞进行平面培养,2代之后进行分类培养,实验组进行电流刺激,并给予相同的刺激时间和刺激强度,对照组未进行电流刺激。之后在不同时间点进行细胞增殖、分化检测及BMP-2蛋白表达分析。
结果：实验组8 d内光镜下可见大量成骨细胞增殖,成骨细胞呈多形性改变,6～8 d细胞内出现少量钙化点,而对照组骨细胞增殖缓慢。实验组分化明显,碱性磷酸酶及钙结节染色阳性,碱性磷酸酶定量分析显示逐渐增加。实验组经免疫组化分析显示BMP-2量逐渐增加。
结论：电刺激可促进成骨细胞的增殖分化而达到实现细胞数目的短时间增加,细胞内部经过免疫组化染色后显示BMP-2表达增加。 |
| [关键词]:成骨细胞 电刺激 细胞增殖 骨基质形成蛋白(BMP-2) 动物实验 |
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| Proliferation,differentiation and BMP-2 expression of osteoblasts cultured in the plane under suitable electrical stimulation |
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Abstract:
Objective: To observe proliferation and differentiation of osteoblasts cultured in the plane on appropriate electrical stimulation,to specify whether it promote the proliferation,and observe expression of BMP-2 on electrical stimulation.
Methods:Osteoblasts were extracted from the skull of rabbit offspring and cultured. Cells after the 2nd generations were cultured. In experimental group,cells had electrical stimulation,and same stimulation time and intensity were given. In control group cells had not electrical stimulation. The proliferation and differentiation were detected at different time,and BMP-2 protein expression was analyzed.
Results:The cell morphology of experimental group in 8 days under the light microscope was observed and showed a lot of proliferation of osteoblasts,pleomorphic changes,in 6 to 8 days a small amount of Calcified spots was also observed;while in the control group,proliferation was slower. Differentiation of the experimental group was significantly,alkaline phosphatase staining and calcium nodules were positive,quantitative analysis of alkaline phosphatase increaseed significantly. Experimental group showed that BMP-2 was gradually increased by immunohistochemistry analysis.
Conclusion:Electrical stimulation can promote the proliferation and differentiation of osteoblasts and achieved the increasement the number of cells in short-term,intracellular staining by immunohistochemistry showed the increasement in expression of BMP-2. |
| KEYWORDS:Osteoblasts Electric stimulation Cell proliferation Bone morphogenetic protein (BMP-2) Animal experimentation |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 王伟,李秀翠,程少文,陈庆玉,彭磊,林忠勤,张伟,寇冬权.适宜电刺激对成骨细胞平面培养下细胞增殖分化及BMP-2表达的实验研究[J].中国骨伤,2012,25(7):587~591 |
| 英文格式: | WANG Wei,LI Xiu-cui,CHENG Shao-wen,CHEN Qing-yu,PENG Lei,LIN Zhong-qin,ZHANG Wei,KOU Dong-quan.Proliferation,differentiation and BMP-2 expression of osteoblasts cultured in the plane under suitable electrical stimulation[J].zhongguo gu shang / China J Orthop Trauma ,2012,25(7):587~591 |
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