| 补肾活血法防治大鼠膝骨性关节炎的实验研究 |
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Received:November 22, 2011
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| 作者 | Author | 单位 | Unit | E-Mail |
| 季卫锋 |
JI Wei-feng |
浙江中医药大学骨痹研究室分子基因实验室, 浙江 杭州 310006
浙江中医药大学附属第一医院骨伤科 |
Department of Molecular Pharmacology, Laboratory of Molecular Genetics, Institute of Gubi Syndrome, Zhejiang University of Traditional Chinese Medicine, Hangzhou 310006, Zhejiang, China |
jiweifeng@zjtcm.net |
| 施伟峰 |
SHI Wei-feng |
浙江中医药大学骨痹研究室分子基因实验室, 浙江 杭州 310006 |
Department of Molecular Pharmacology, Laboratory of Molecular Genetics, Institute of Gubi Syndrome, Zhejiang University of Traditional Chinese Medicine, Hangzhou 310006, Zhejiang, China |
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| 陈林 |
CHEN Lin |
浙江中医药大学骨痹研究室分子基因实验室, 浙江 杭州 310006 |
Department of Molecular Pharmacology, Laboratory of Molecular Genetics, Institute of Gubi Syndrome, Zhejiang University of Traditional Chinese Medicine, Hangzhou 310006, Zhejiang, China |
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| 马镇川 |
MA Zhen-chuan |
浙江中医药大学附属第一医院骨伤科 |
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| 袁小凤 |
YUAN Xiao-feng |
浙江中医药大学生命科学学院 |
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| 许健 |
XU Jian |
浙江中医药大学生命科学学院 |
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| 厉驹 |
LI Ju |
浙江中医药大学附属第一医院骨伤科 |
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| 童培建 |
TONG Pei-jian |
浙江中医药大学骨痹研究室分子基因实验室, 浙江 杭州 310006
浙江中医药大学附属第一医院骨伤科 |
Department of Molecular Pharmacology, Laboratory of Molecular Genetics, Institute of Gubi Syndrome, Zhejiang University of Traditional Chinese Medicine, Hangzhou 310006, Zhejiang, China |
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| 肖鲁伟 |
XIAO Lu-wei |
浙江中医药大学骨痹研究室分子基因实验室, 浙江 杭州 310006
浙江中医药大学附属第一医院骨伤科 |
Department of Molecular Pharmacology, Laboratory of Molecular Genetics, Institute of Gubi Syndrome, Zhejiang University of Traditional Chinese Medicine, Hangzhou 310006, Zhejiang, China |
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| 期刊信息：《中国骨伤》2012年25卷，第3期，第246-250页 |
| DOI：10.3969/j.issn.1003-0034.2012.03.018 |
| 基金项目:浙江省中医药科研项目(编号:2008yb002),浙江省教育厅研究生科技创新项目(编号:YK2009066) |
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目的:探讨补肾活血法、活血法、补肾法、祛风法对SD大鼠膝骨性关节炎血液流变学及滑液IL-1β、TNF-α的作用及其机制,明确筋痹到骨痹的演变及中药疗效的比较。
方法:取体重为(200±15) g的3月龄雄性SD大鼠180只,采用木瓜蛋白酶大鼠膝关节腔注射的方法制作膝骨性关节炎动物模型,随机分为活血组、预防组、祛风组、补肾组、补肾活血组和模型组。于中药灌胃后30、60、90 d分批处死动物后进行各种指标的检测,包括对大鼠的精神状态、活动度、皮毛、体重、关节肿胀情况,大体形象,血流变学,炎症指标和HE染色的病理切片。
结果:大鼠造模成功后出现膝关节瘀血肿胀,爬行困难。中药治疗30,60,90 d后对比,补肾活血汤组血液流变学各项指标较模型组差异有统计学意义;病理组织学表现软骨面较模型组光滑,完整,软骨细胞排列整齐;白细胞介素-1β(IL-1β)及肿瘤坏死因子α(TNF-α)含量明显低于模型组。模型组早期IL-1β及TNF-α含量高于晚期。
结论:膝骨性关节炎早期主要表现为滑膜炎症,早期炎症反应较晚期严重。补肾活血方能抑制大鼠膝关节软骨损伤,改善血液循环及抑制局部炎症反应。早期活血方、补肾活血方均有一定疗效,但晚期补肾活血方疗效较活血方,补肾方更显着。 |
| [关键词]:骨关节炎 膝关节 大鼠 血液流变学 |
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| Experimental study on invigorating kidney and activating blood(补肾活血) on preventing and curing SD rats with knee osteoarthritis |
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Abstract:
Objective:To explore the effects and mechanism of invigorating kidney and activating blood(补肾活血),invigorating kidney(补肾) and expelling wind(祛风) on hemorheology,IL-1β and TNF-α of SD rats with knee osteoarthritis,then definite the evolution of muscle certified turning into heumatism and compare the effect of Chinese herbal.
Methods:One hundred and eighty SD rats with 3-month-old(each weight was 185 to 215 g) received intra-articular injection of papain solution for establishing knee OA models. All rats were randomly divided into activating blood group(活血组),preventing group,expelling wind group(祛风组),invigorating kidney group(补肾组),invigorating kidney and activating blood group(补肾活血)and model group. Laboratory indexes were obtained at the 30th,60th,90th days after gastric perfusion,which including state of mind,activity,fur,weight,joint swelling,largely image,hemorheology,inflammation and HE pathological appearance.
Results:After operation,rats appeared blood stasis and swelling and difficulty crawling. There was significant difference of hemorheology in invigorating kidney and activating blood group(补肾活血);the content of IL-1β and TNF-α was obviously lower than model group(P<0.05).While the content of IL-1β and TNF-α on the early stage was obviously higher than late stage(P<0.05).
Conclusion:Knee osteoarthritis mainly show synovial inflammation at the early stage,inflammation at early stage is more severe than late; invigorating kidney and activating blood decoction(补肾活血方) can inhibit the knee cartilage injury,improve blood circulation and prevent local inflammatory reaction. Activating blood decoction(活血方) and invigorating kidney and activating blood Decoction(补肾活血方) have certain curative effect in early time,but the effects of invigorating kidney and activating blood Decoction(补肾活血方) is more effective than other on the late stage. |
| KEYWORDS:Osteoarthritis Knee joint Rats Hemorheology |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 季卫锋,施伟峰,陈林,马镇川,袁小凤,许健,厉驹,童培建,肖鲁伟.补肾活血法防治大鼠膝骨性关节炎的实验研究[J].中国骨伤,2012,25(3):246~250 |
| 英文格式: | JI Wei-feng,SHI Wei-feng,CHEN Lin,MA Zhen-chuan,YUAN Xiao-feng,XU Jian,LI Ju,TONG Pei-jian,XIAO Lu-wei.Experimental study on invigorating kidney and activating blood(补肾活血) on preventing and curing SD rats with knee osteoarthritis[J].zhongguo gu shang / China J Orthop Trauma ,2012,25(3):246~250 |
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