| 黄芪与当归对体外培养糖尿病骨髓干细胞增殖和血管内皮生长因子表达的影响 |
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Received:November 09, 2010
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| 期刊信息：《中国骨伤》2011年24卷，第8期，第652-655页 |
| DOI：10.3969/j.issn.1003-0034.2011.08.009 |
| 基金项目:浙江省中医药项目(编号:Y2007CA081);温州市科技局项目(编号:Y0090127) |
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目的: 观察黄芪、当归及二者合用对体外培养大鼠骨髓干细胞增殖的影响,并探讨其可能机制.
方法: 自2009年7月至2010年2月,选择5只雄性200～220 g SD大鼠高糖高脂饲料喂养4周后,给予链脲佐菌素(STZ)按照30 mg/kg腹腔内注射2次诱发2型糖尿病,1周后检测血糖≥16.7 mmol/L为2型糖尿病造模成功.密度梯度法分离5只大鼠骨髓干细胞,分为空白对照组(A组)、黄芪组(B组)、当归组(C组)、黄芪当归组(D组).空白对照组加入无血清DMEM 100 μl,黄芪组、当归组、黄芪当归组分别加入等量的用无血清DMEM配制的黄芪煎液、当归煎液、黄芪当归合煎液,终浓度分别为黄芪1 100 mg/L,当归1 100 mg/L,黄芪当归组含黄芪1 100 mg/L、当归220 mg/L.各组细胞培养14 d后,采用MTT法测定细胞增殖,ELISA法检测细胞培养上清液VEGF蛋白浓度,Western-Blot检测骨髓干细胞VEGF蛋白表达.
结果: 与空白对照组比较,黄芪组和黄芪当归组的骨髓干细胞增殖水平、上清液VEGF蛋白浓度、骨髓干细胞VEGF蛋白表达水平均明显增高(P<0.05 或P<0.01),且黄芪当归组的以上作用均较黄芪组明显增强(P<0.05或P<0.01).
结论: 单用黄芪或其与当归合用均具有刺激体外培养骨髓干细胞增殖的作用,可能与其促进VEGF蛋白表达增加有关,但单用当归无上述作用. |
| [关键词]:黄芪 当归 血管内皮细胞生长因子类 细胞增殖 骨髓 |
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| Effects of Astragalus and Angelica on bone marrow stem cells proliferation and VEGF protein expression in vitro |
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Abstract:
Objective: To observe the effects of Astragalus and Angelica on bone marrow stem cells(BMSC)proliferation in vitro and investigate its possible mechanism.
Methods: Five 200 to 220 g SD rats were fed with a high fat diet for 4 weeks and given 30 mg/kg streptozotocin (STZ) twice develop typeⅡdiabetes from July 2009 to February 2010. The rats with blood glucose concentrations of 16.7 mmol/L or more were considered diabetic. Bone Marrow Stem Cells(BMSC)were collected and isolated by density gradient centrifugation. The BMSC were divided into 4 groups,including empty control group,Astragalus group,Angelica group and Astragalus plus Angelica group. DMEM of 100 μl was added in empty control group. DMEM of 100 μl containing Astragalus (1 100 mg/L),Angelica(1 100 mg/L) and Astragalus(1 100 mg/L) combine with Angelica(220 mg/L) were added in Astragalus group,Angelica group and Astragalus plus Angelica group respectively. The cell proliferation was detected by MTT method,and the concentration of VEGF in the supernatant was determined by ELISA. The VEGF expression was analyzed by Western Blot after 14 days incubation.
Results: The BMSC proliferation and the VEGF concentration in the supernatant and the BMSC VEGF protein expression significantly increased in Astragalus group and Astragalus plus Angelica group compared to those of empty control group(P<0.05 or P<0.01). The above effects were more strong in Astragalus plus Angelica group(P<0.05).
Conclusion: Astragalus with Angelica or used separately could promote BMSC proliferation. The mechanism might induce the VEGF protein expression in BMSC. And the independent use of Angelica has no above effect. |
| KEYWORDS:Astragalus membranaceus Angelica sinensis Vascular endothelial growth factors Cell proliferation Bone marrow |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 沈斌,陈雷,周凯,金可可.黄芪与当归对体外培养糖尿病骨髓干细胞增殖和血管内皮生长因子表达的影响[J].中国骨伤,2011,24(8):652~655 |
| 英文格式: | SHEN Bin,CHEN Lei,ZHOU Kai,JIN Ke-ke.Effects of Astragalus and Angelica on bone marrow stem cells proliferation and VEGF protein expression in vitro[J].zhongguo gu shang / China J Orthop Trauma ,2011,24(8):652~655 |
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