| 利水渗湿法对髓核自身免疫模型大鼠血清IgG、IgM及IL-1β、IL-8的影响 |
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Received:November 10, 2010
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| 作者 | Author | 单位 | Unit | E-Mail |
| 朱立国 |
ZHU Li-guo |
中国中医科学院望京医院脊柱二科, 北京 100102 |
Department of the Second Spine, Wangjing Hospital of China Academy of Traditional Chinese Medicine Sciences, Beijing 100102, China |
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| 陈忻 |
CHEN Xin |
中国中医科学院望京医院脊柱二科, 北京 100102 |
Department of the Second Spine, Wangjing Hospital of China Academy of Traditional Chinese Medicine Sciences, Beijing 100102, China |
15101161150@139.com |
| 于杰 |
YU Jie |
中国中医科学院望京医院脊柱二科, 北京 100102 |
Department of the Second Spine, Wangjing Hospital of China Academy of Traditional Chinese Medicine Sciences, Beijing 100102, China |
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| 高景华 |
GAO Jing-hua |
中国中医科学院望京医院脊柱二科, 北京 100102 |
Department of the Second Spine, Wangjing Hospital of China Academy of Traditional Chinese Medicine Sciences, Beijing 100102, China |
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| 王尚全 |
WANG Shang-quan |
中国中医科学院望京医院脊柱二科, 北京 100102 |
Department of the Second Spine, Wangjing Hospital of China Academy of Traditional Chinese Medicine Sciences, Beijing 100102, China |
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| 冯敏山 |
FENG Min-shan |
中国中医科学院望京医院脊柱二科, 北京 100102 |
Department of the Second Spine, Wangjing Hospital of China Academy of Traditional Chinese Medicine Sciences, Beijing 100102, China |
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| 银河 |
YIN He |
北京中医药大学基础医学院 |
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| 期刊信息：《中国骨伤》2011年24卷，第4期，第327-331页 |
| DOI：10.3969/j.issn.1003-0034.2011.04.018 |
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目的：探讨利水渗湿法对大鼠髓核引起的自身免疫及免疫炎症反应的影响, 为腰椎间盘突出症的中药免疫治疗提供依据。
方法：将40只雄性Wistar大鼠按体重分层随机分组的方法分为假手术组(A组)、模型对照组(B组)、秋水仙碱片组(C组)、轻腰汤加味组(D组), 每组10只。B、C、D组进行造模：通过手术将大鼠尾椎髓核移植于臀部肌中, 造成自身免疫及免疫炎症反应；A组只做假手术处理。各组大鼠于术后第3天开始灌胃, A组和B组用蒸馏水(10 ml/kg), C组和D组分别用秋水仙碱片混悬液(10 ml/kg, 0.01 mg/ml)和轻腰汤加味水煎液(10 ml/kg, 1.035 g/ml), 每天1次, 连续18 d.于术后21 d处死动物, 应用ELISA法检测血清中免疫球蛋白G(IgG)、免疫球蛋白M(IgM)及白介素-1β(IL-1β)、白介素-8(IL-8)的含量, 并观察移植髓核周围组织的病理变化。
结果：与A组比较, B组血清IgG、IgM及IL-1β、IL-8 含量明显升高, 差异有统计学意义(P<0.01);与B组比较, C、D组血清IgG、IgM及IL-1β、IL-8含量明显降低, 差异有统计学意义(P<0.05或P<0.01);病理切片显示, A组手术部位无明显免疫炎症反应, C、D组移植髓核周围组织局部免疫炎症反应与B组比较明显减轻。
结论：利水渗湿法对大鼠髓核引起的自身免疫及免疫炎症反应, 具有较好的抑制作用。 |
| [关键词]:腰椎 椎间盘移位 自身免疫 炎症 利水渗湿 |
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| Effect of removing dampness and promoting diuresis(利水渗湿)method on IgG, IgM and IL-1β, IL-8 in serum of rats with autoimmunity induced by nucleus pulposus |
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Abstract:
Objective: To explore the effects of removing dampness and promoting diuresis(利水渗湿)method on autoimmune and immuno-inflammatory response caused by nucleus pulposus of rats, in order to provide the basis for the treatment of lumbar disc herniation with Chinese medical immunotherapy.
Methods: Forty male Wistar rats were devided into 4 groups randomly according to body weight layer:sham operation group(group A), model contrast group(group B), colchicine tablets group(group C), modified Qingyao decoction(轻腰汤加味) group (group D). There were 10 rats in each group. Nucleus pulposus of coccygeal vertebra was transplanted to the gluteal muscle by operation in groups B, C, D, which can lead to autoimmune and immuno-inflammatory response of rats;the rats of group A were only treated with sham operation. At the 3rd day after operation, the rats were fed through intragastric administration, the group A and B with distilled water(10 ml/kg), the group C and D respectively with suspension of colchicine tablets(10 ml/kg, 0.01 mg/ml) and water-decocted liquid of modified Qingyao decoction(轻腰汤加味)(10 ml/kg, 1.035 g/ml), once a day and continuous medication for 18 days. All rats were killed at the 21th day after operation. The immunoglobulin G(IgG), immunoglobulin M(IgM) and interleukin-1β(IL-1β), interleukin-8(IL-8) level in serum of different groups were detected by ELISA method. At the same time, surrounding tissues of the transplanted nucleus pulposus were observed by pathological section.
Results: The level of lgG, IgM, IL-1β, IL-8 in serum of group B was significantly higher than that of group A(P<0.01), while the level of lgG, IgM, IL-1β, IL-8 in serum of group C, D was significantly lower than that of group B(P<0.05 or P<0.01). Moreover, pathological section indicated that immuno-inflammatory response was hardly found in surgical site of group A, while local immuno-inflammatory response of surrounding tissues of the transplanted nucleus pulposus of group C and D was much lighter than that of group B.
Conclusion: Removing dampness and promoting diuresis(利水渗湿)method could inhibit autoimmune and immuno-inflammatory response caused by nucleus pulposus of rats. |
| KEYWORDS:Lumbar vertebrae Intervertebral disk displacement Autoimmunity Inflammation Diuresis-removing dampness |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 朱立国,陈忻,于杰,高景华,王尚全,冯敏山,银河.利水渗湿法对髓核自身免疫模型大鼠血清IgG、IgM及IL-1β、IL-8的影响[J].中国骨伤,2011,24(4):327~331 |
| 英文格式: | ZHU Li-guo,CHEN Xin,YU Jie,GAO Jing-hua,WANG Shang-quan,FENG Min-shan,YIN He.Effect of removing dampness and promoting diuresis(利水渗湿)method on IgG, IgM and IL-1β, IL-8 in serum of rats with autoimmunity induced by nucleus pulposus[J].zhongguo gu shang / China J Orthop Trauma ,2011,24(4):327~331 |
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