| IL-12诱导类风湿关节炎和骨关节炎外周血T淋巴细胞STAT4活化状态的比较 |
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Received:December 11, 2010
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| 作者 | Author | 单位 | Unit | E-Mail |
| 孙振杰 |
SUN Zhen-jie |
北京市第六医院骨科, 北京 100007 |
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| 张育军 |
ZHANG Yu-jun |
北京大学人民医院中心实验室 |
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| 孙铁铮 |
SUN Tie-zheng |
北京大学人民医院关节病诊疗研究中心, 北京 100035 |
Arthritis Clinic and Research Center, People's Hospital of Peking University, Beijing 100035, China |
suntiezheng1973@yahoo.com.cn |
| 熊奡 |
XIONG Ao |
深圳市北京大学深圳医院骨科 |
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| 刘瑞波 |
LIU Rui-bo |
北京市第六医院骨科, 北京 100007 |
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| 吕厚山 |
LV Hou-shan |
北京大学人民医院关节病诊疗研究中心, 北京 100035 |
Arthritis Clinic and Research Center, People's Hospital of Peking University, Beijing 100035, China |
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| 期刊信息：《中国骨伤》2011年24卷，第4期，第295-298页 |
| DOI：10.3969/j.issn.1003-0034.2011.04.008 |
| 基金项目:首都医学发展基金资助课题(编号：TS199923) |
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目的：比较类风湿关节炎和骨关节炎外周血T淋巴细胞在IL-12作用下诱导STAT4酪氨酸磷酸化水平的差异。
方法：2007年5月至2009年8月分离提纯20例类风湿关节炎患者[均为女性；年龄28～55岁, 平均(45.0±13.0)岁]和20例骨关节炎患者[均为女性；年龄55～75岁, 平均(67.0±9.6)岁]的外周血T淋巴细胞, 流式细胞仪进行T淋巴细胞纯度鉴定。用50 ng/ml IL-12分别作用0、10、30、60 min后, 提取蛋白进行浓度测量, 然后进行Western Blot检测, 经蛋白条带灰度扫描, 比较IL-12作用各时间点类风湿关节炎和骨关节炎T淋巴细胞STAT4酪氨酸磷酸化水平的差异。
结果：经过对外周血单核细胞的分离纯化, T细胞纯度达到91%以上；IL-12在对骨关节炎和类风湿关节炎T细胞作用30 min后达到活化峰值, 在各个时间点(10、30、60 min)类风湿关节炎外周血T淋巴细胞活化水平均高于骨关节炎。
结论：类风湿关节炎外周血T淋巴细胞在IL-12作用下STAT4异常活化。 |
| [关键词]:关节炎，类风湿 淋巴细胞 白细胞介素-12 |
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| Comparison of the status of STAT4 tyrosine phosphorylation in peripheral T-lymphocytes induced by IL-12 in rheumatoid arthritis and osteoarthritis |
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Abstract:
Objective: To compare the levels of STAT4 tyrosine phosphorylation in peripheral T-lymphocytes induced by IL-12 in rheumatoid arthritis(RA) and osteoarthritis(OA).
Methods: From May 2007 to August 2009, peripheral blood mononuclear cells(PBMCs) were isolated from RA patients[RA group, all the cases were female, the age was from 28 to 55 years with an average of (45.0±13.0) years] and OA patients[OA group, all the cases also were female;the age was from 55 to 75 years with an average of (67.0±9.6) years]. The purity of T-lymphocytes from PBMCs was accredited by flow cytometry. The IL-12 of 50 ng/ml added in T-lymphocytes, the levels of STAT4 tyrosine phosphorylation were detected by western blot after different time intervals(0, 10, 30, 60 min).
Results: The purity of T-lymphocytes were above 91% through diremption and depuration for peripheral blood monouclear cells. The levels of STAT4 tyrosine phosphorylation in T-lymphocytes from RA induced by IL-12 were higher than that from OA in the different times(10, 30, 60 min);after 30 min, its levels from RA and OA achieved to crest value.
Conclusion: STAT4 in peripheral T-lymphocytes of rheumatoid arthritis was more easily to be activated than osteoarthritis. |
| KEYWORDS:Arthritis, rheumatoid Lymphocytes Interleukin-12 |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 孙振杰,张育军,孙铁铮,熊奡,刘瑞波,吕厚山.IL-12诱导类风湿关节炎和骨关节炎外周血T淋巴细胞STAT4活化状态的比较[J].中国骨伤,2011,24(4):295~298 |
| 英文格式: | SUN Zhen-jie,ZHANG Yu-jun,SUN Tie-zheng,XIONG Ao,LIU Rui-bo,LV Hou-shan.Comparison of the status of STAT4 tyrosine phosphorylation in peripheral T-lymphocytes induced by IL-12 in rheumatoid arthritis and osteoarthritis[J].zhongguo gu shang / China J Orthop Trauma ,2011,24(4):295~298 |
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