| 不同治法方药对激素性股骨头坏死的骨组织
形态学影响 |
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Received:April 12, 2010
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| 作者 | Author | 单位 | Unit | E-Mail |
| 万蓉 |
WAN Rong |
中国中医科学院中药研究所, 北京 100700 |
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| 林诗富 |
LIN Shi-fu |
中国中医科学院望京医院 |
Institute of Chinese Materia Medicine, China Academy of Chinese Medical Sciences, Beijing 100700, China |
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| 林娜 |
LIN Na |
中国中医科学院中药研究所, 北京 100700 |
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linna888@163.com |
| 肖春生 |
XIAO Chun-sheng |
中国中医科学院望京医院 |
Institute of Chinese Materia Medicine, China Academy of Chinese Medical Sciences, Beijing 100700, China |
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| 孔祥英 |
KONG Xiang-ying |
中国中医科学院中药研究所, 北京 100700 |
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| 陈卫衡 |
CHEN Wei-heng |
中国中医科学院望京医院 |
Institute of Chinese Materia Medicine, China Academy of Chinese Medical Sciences, Beijing 100700, China |
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| 期刊信息：《中国骨伤》2010年23卷，第12期，第915-919页 |
| DOI：10.3969/j.issn.1003-0034.2010.12.010 |
| 基金项目:国家自然科学基金面上项目(编号：30472131) |
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目的：观察比较健脾化痰、活血通络与补肾壮骨、活血通络两种不同治法方药对激素性股骨头坏死形态学影响。
方法：健康来航鸡64只，随机分为正常组，模型组，健脾化痰、活血通络组(简称健脾组)和补肾壮骨、活血通络组(简称补肾组),每组16只。分别于实验后8、16周取股骨头，采用HE 和Masson染色方法观察其组织病理学形态。
结果：与正常组相比，模型组髓腔内脂肪细胞面积增多(t=-12.9,P<0.01),股骨头内不成熟胶原面积明显增大(t=-2.0,P<0.05) ,骨小梁内空骨陷窝率显着增高(t=-3.7,P<0.05).与模型组比较，2个给药组均能明显减少髓腔内脂肪细胞面积(F=26.8,13.5,P<0.01)和股骨头内不成熟胶原面积(F=4.6,P<0.01).
结论：健脾化痰、活血通络和补肾壮骨、活血通络两种不同治法方药均可减轻激素性股骨头坏死的组织形态病理学改变，前者发挥药效的时间相对较早。 |
| [关键词]:股骨头坏死 病理学 健脾 补肾 活血通络 |
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| Effects of different Chinese drugs on bone histomorphology of hormone induced femoral head necrosis |
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Abstract:
Objective: To evaluate and compare the histopathology effects and mechanisms of the methods of “invigorating spleen to remove phlem & promoting blood circulation to remove meridian obstruction” and “invigorate the kidney & promoting blood circulation to remove meridian obstruction” preventing hormone induced femoral head necrosis in hens.
Methods: Sixty-four healthy hens were randomly divided into 4 groups:blank control group,model group,Jianpi group(with therapeutics of invigorating spleen to remove phlem),Bushen group (with the effect of warming kidney for duresis). All hens were injected intramuscularly with Medrat once a week for 16 weeks but normal saline in blank control group. Bilateral femoral heads were dissected on 8 weeks or 16 weeks. Paraffin tissue sections were prepared to detect histopathologic change with hematoxylin and eosin,or mason stainning.
Results: Histological analysis showed that Huogu recipe I and Huogu recipeⅡcan promote osteogenesis and repair osteonecrosis,increase blood circulation of bone marrow,and inhibit pimelosis of bone marrow. Compared with blank control group,the areas of adipose cells increased significantly(t=-12.9,P<0.01),the area of immature collagen increased significantly(t=-2.0,P<0.05) and the ratio of empty lacuna in medullary cavity(t=-3.7,P<0.05). Compared with model group,both the area of adipose cells and the ratio of empty lacuna decreased in Jianpi group and Bushen group(F=26.8,13.5,P<0.01),so it was with the Bushen group immature collagen(F=4.6,P<0.01).
Conclusion: Both the methods of “invigorating spleen to remove phlem & promoting blood circulation to remove meridian obstruction” and “invigorate the kidney & promoting blood circulation to remove meridian obstruction ”can prevent hormone induced femoral head necrosis. The time taking effect in the method of “invigorating spleen to remove phlem” was shorter. |
| KEYWORDS:Femur head necrosis Pathology Strengthening spleen Reinforcing kidney Activate blood dredging collaterals |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 万蓉,林诗富,林娜,肖春生,孔祥英,陈卫衡.不同治法方药对激素性股骨头坏死的骨组织
形态学影响[J].中国骨伤,2010,23(12):915~919 |
| 英文格式: | WAN Rong,LIN Shi-fu,LIN Na,XIAO Chun-sheng,KONG Xiang-ying,CHEN Wei-heng.Effects of different Chinese drugs on bone histomorphology of hormone induced femoral head necrosis[J].zhongguo gu shang / China J Orthop Trauma ,2010,23(12):915~919 |
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