| 小针刀对肌张力增高大鼠脊髓和背根神经节内P物质影响的研究 |
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Received:May 25, 2010
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| 期刊信息：《中国骨伤》2010年23卷，第9期，第692-695页 |
| DOI：10.3969/j.issn.1003-0034.2010.09.016 |
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目的：探讨骨骼肌受压后初级感觉神经元兴奋性递质(substance P,SP)在背根神经节(dorsal root ganglion,DRG)内的合成、脊髓中枢端末梢的释放机制，观察小针刀针刺的影响。
方法：采用大鼠股薄肌70 kPa受压模型。24只大鼠随机均分为3组，分别为空白组、压迫组、针刀组。除空白组6只外，其余18只大鼠左侧设为压迫组，右侧设为针刀组，组内分1、2、3 d不同组，每组6只。两组同时进行70 kPa压强压迫，每天压迫1次，每次2 h.同时于造模后对针刀组股薄肌进行小针刀针刺。用免疫组织化学方法结合图像分析技术检测各组大鼠股薄肌受压后及针刺后背根神经节和相应脊髓节段内P物质的表达变化。
结果：在DRG中，空白组针刀侧阳性反应明显强于对侧，P<0.01;针刀1、2 d 组SP表达整合光密度(integrated optical density,IOD)较压迫组低，P<0.05.在脊髓中，与压迫组比较，针刀1、3 d组SP阳性表达area和IOD数值较高，P<0.01;2 d组两指标则低于对侧。
结论：小针刀针刺可明显减低骨骼肌受压后DRG内疼痛物质SP的合成，SP在中枢末梢的释放在短期内(3 d)未见明显变化。 |
| [关键词]:P物质 神经节 脊髓 疼痛 小针刀 |
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| Effects of small needle knife on the substance P in the dorsal root ganglion and spinal cord of rats |
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Abstract:
Objective: To study the mechanism of synthesis of substance P (SP) in the dorsal root ganglion(DRG) and the release of it in the dorsal horn of the spinal cord of rats after compression of skeletal muscle,and to observe the influence of small needle knife.
Methods: Sustained pressure of 70 kPa was applied to rats,muscular tissues for 2 hours. The rats were divided into three groups:normal,control and experiment group respectively. In all rats except the six normal ones,the lower legs were compressed once one day. The left leg was considered as the control group,the right left was experiment group,which were divided into the 1st day,the 2nd day and the 3rd day within the two groups. Experiment group was treated with small needle knife after the muscular tissue was compressed. After completing the stimulation,the DRG related to the muscle and part of spinal cord were removed for the qualification of SP-like immunoreactivity using immunohistochemistry. The dark brown stains on the DRG and on the REXed laminaeⅠandⅡ in the dorsal horn of the spinal cord were counted by Image-Pro Plus software.
Results: SP-like immunoreactivity in the side treated by the small needle knife was enhanced comparing with the counterpart in DRG in normal group(P<0.01). The integrated optical density of SP like immunoreactivity of the DRG in the experiment group were significantly reduced compared with the control group(P<0.05). However,the release of SP from spinal cord in experiment group was lower than that in the control group at the 1st day and the 3rd day(P<0.01),with the opposite result of the 2nd day.
Conclusion: Based on the fact that SP is a nociceptive neurotransmitter,the present study suggests that tension relaxation by small needle knife reduces expression of SP in the DRG,and shows no effects on the release of SP from the spinal cord in short term(3 days). |
| KEYWORDS:Substance P Ganglia Spinal cord Pain Small needle knife |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 王金荣,王永志,董福慧,钟红刚,王德龙,王萱.小针刀对肌张力增高大鼠脊髓和背根神经节内P物质影响的研究[J].中国骨伤,2010,23(9):692~695 |
| 英文格式: | WANG Jin-rong,WANG Yong-zhi,DONG Fu-hui,ZHONG Hong-gang,WANG De-long,WANG Xuan.Effects of small needle knife on the substance P in the dorsal root ganglion and spinal cord of rats[J].zhongguo gu shang / China J Orthop Trauma ,2010,23(9):692~695 |
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