| rhBMP-2在老年兔后外侧脊柱融合中作用的实验研究 |
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Received:June 02, 2009
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| 作者 | Author | 单位 | Unit | E-Mail |
| 段兴威 |
DUAN Xing-wei |
吉林大学中日联谊医院骨科,吉林 长春 130033 |
Department of Orthopaedics,China-Japan Union Hospital,Jilin University,Changchun 130033,Jilin,China |
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| 武汉 |
WU Han |
吉林大学中日联谊医院骨科,吉林 长春 130033 |
Department of Orthopaedics,China-Japan Union Hospital,Jilin University,Changchun 130033,Jilin,China |
drwuhan@yahoo.com.cn |
| 谷长跃 |
GU Chang-yue |
吉林大学中日联谊医院骨科,吉林 长春 130033 |
Department of Orthopaedics,China-Japan Union Hospital,Jilin University,Changchun 130033,Jilin,China |
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| 于彦 |
YU Yan |
澳大利亚新南威尔士大学威尔士王子医院骨科研究室 |
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| 杨小玉 |
YANG Xiao-yu |
吉林大学中日联谊医院骨科,吉林 长春 130033 |
Department of Orthopaedics,China-Japan Union Hospital,Jilin University,Changchun 130033,Jilin,China |
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| 魏君 |
WEI Jun |
吉林大学中日联谊医院骨科,吉林 长春 130033 |
Department of Orthopaedics,China-Japan Union Hospital,Jilin University,Changchun 130033,Jilin,China |
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| 段德生 |
DUAN De-sheng |
吉林大学中日联谊医院骨科,吉林 长春 130033 |
Department of Orthopaedics,China-Japan Union Hospital,Jilin University,Changchun 130033,Jilin,China |
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| 期刊信息：《中国骨伤》2010年23卷，第1期，第28-32页 |
| DOI：10.3969/j.issn.1003-0034.2010.01.009 |
| 基金项目:国家自然科学基金资助项目(编号：30772209) |
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目的：通过对老年和青年兔采用基因重组人骨形态发生蛋白-2(rhBMP-2)和自体骨移植进行腰椎后外侧横突间融合手术实验研究，揭示rhBMP-2在老年动物脊柱融合中的作用效果。
方法：采用24只雌性新西兰大白兔，体重3.5～4.5 kg,6月龄(青年)和24月龄(老年)各12只。随机分成4组：①青年自体髂骨移植组[ICBG(Y),6只];②青年rhBMP-2组[rhBMP-2(Y),6只];③老年自体髂骨移植组[ICBG(O),6只];④老年rhBMP-2组[rhBMP-2(O),6只].同一天施行腰椎后外侧横突间融合术。rhBMP-2和自体髂骨分别植入两侧L5-L5横突间。术后3、6周分别处死一半。结果采用手动触诊，X线摄片，CT三维扫描和组织学评估。
结果：X线片及三维CT显示rhBMP-2组的融合率均高于自体髂骨移植组，而青年各组融合率比老年组高，各组之间比较差异具有统计学意义(P<0.05).CT三维重建显示由于rhBMP-2是液态，有流动性，成骨范围较大，不局限于计划融合区。组织学显示老年rhBMP-2组成骨面积较青年rhBMP-2组小，而且骨小梁结构也不如后者密集。
结论：与自体髂骨移植比较，rhBMP-2可显着增强老年兔脊柱后外侧融合率和新骨生成量，加快融合时间，可以取代自体髂骨移植。rhBMP-2促进骨再生能力受年龄影响。 |
| [关键词]:骨形态发生蛋白质类 脊柱融合术 骨质疏松 骨移植 兔 |
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| Experiment of rhBMP-2 in posterolateral spinal fusion in aged rabbits |
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Abstract:
Objective: Posterolateral intertransverse process fusion was performed in aged and young adult female rabbits lumbar spine using recombinant human bone morphogenetic protein-2(rhBMP-2 ) and autograft to reveal the function of rhBMP-2 on spinal fusion on aged animals.
Methods: A total of 24 female New Zealand white rabbits included 12 young adult of 6 months and 12 aged of 2-year-old,was divided into 4 groups:①young adult autologous iliac crest bone group[ICBG(Y),n=6);②young adult rhBMP-2/absorbable collagen sponge(ACS) group[BMP-2(Y),n=6];③aged autologous iliac crest bone group[ICBG(O),n=6];④aged rhBMP-2/ACS group[BMP-2(O),n=6]. All were underwent posterolateral fusion in same day. rhBMP-2 and autologous iliac crest bone was implant bilateral L5-L6 intertransverse processes,respectively. Half of the rabbits were sacrificed at 3,6 weeks following surgery,respectively. The results were assessed by manual palpation,radiographs,computed tomographic scans(3D) and histology.
Results: Six weeks after surgery,radiography,computed tomography and histology indicated the different result in healing in the posterolateral fusion using rhBMP-2 compared to ICBG(P<0.05). Aged BMP-2 group showed significantly higher fusion rates than Aged ICBG group.
Conclusion: This study demonstrated rhBMP-2 can increase the posterolateral fusion rate and new bone quality in aged rabbitss than autograft,it may take the place of ICBG. But its role is effected by age. |
| KEYWORDS:Bone morphogenetic protein Spinal fusion Osteoporosis Bone transplantation Rabbits |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 段兴威,武汉,谷长跃,于彦,杨小玉,魏君,段德生.rhBMP-2在老年兔后外侧脊柱融合中作用的实验研究[J].中国骨伤,2010,23(1):28~32 |
| 英文格式: | DUAN Xing-wei,WU Han,GU Chang-yue,YU Yan,YANG Xiao-yu,WEI Jun,DUAN De-sheng.Experiment of rhBMP-2 in posterolateral spinal fusion in aged rabbits[J].zhongguo gu shang / China J Orthop Trauma ,2010,23(1):28~32 |
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