| 祛风止痛胶囊对关节炎模型大鼠抗炎作用及机制的实验研究 |
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Received:August 07, 2009
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| 期刊信息：《中国骨伤》2009年22卷，第11期，第848-851页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
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目的：研究中药祛风止痛胶囊对关节炎模型大鼠的关节炎症、肿胀、结构破坏的影响，探讨其抗炎作用机制。
方法：50只雄性SD大鼠随机分为5组：正常对照组，模型组，祛风止痛胶囊低、中、高剂量组，每组10只。除正常对照组外，其他4组应用Ⅱ型胶原-弗氏完全佐剂诱导大鼠关节炎，用祛风止痛胶囊连续灌胃给药2周进行治疗，测量大鼠后肢足爪体积，观察大鼠踝关节组织病理学变化，并用ELISA方法检测大鼠血清中白细胞介素(IL)-10、肿瘤坏死因子(TNF)-α的含量。
结果：与模型组比较，祛风止痛胶囊降低了大鼠后足爪肿胀程度。大鼠踝关节组织病理学变化显示，祛风止痛胶囊减轻滑膜组织的异常增生，炎性细胞浸润呈剂量依赖性的降低；大鼠血清中促炎细胞因子TNF-α的水平变化不明显，而抑制炎症的细胞因子IL-10水平明显升高。
结论：中药祛风止痛胶囊通过上调抑制炎症的细胞因子IL-10的水平而抑制了大鼠关节炎的滑膜增生和软骨的破坏，提示祛风止痛胶囊可作为类风湿关节炎的治疗药物。 |
| [关键词]:抗风湿药 类风湿性关节炎 白细胞介素10 肿瘤坏死因子α 动物实验 |
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| Anti-inflammatory effect of Qufeng Zhitong(祛风止痛)capsule on the collagen-induced arthritis rat |
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Abstract:
Objective: To investigate the role of anti-inflammatory effect and it's mechansim of Qufeng Zhitong capsule on the collagen-induced arthritis rat through the study of arthritis,joint inflammation and joint erosions.
Methods: Fifty male SD mice were assigned to 5 groups:normal control group,arthritis model group,and Qufeng Zhitong capsule treatment group(including low,medium and high doses),each group contains 10 rats. Arthritis were induced by immunization with type Ⅱcollagen (CII) in complete Freund's adjuvant(CFA). After administered Qufeng Zhitong capsule 2 weeks,the volume of back foot of arthritis rat were detected,and the histology changes of the ankle joints of the arthritis rat were studied. The serum levels of IL-10 and TNF-α were measured by ELISA.
Results: After treated with Qufeng Zhitong capsule,the swelling of the paw of the rats were significantly decreased compared with the contrals. Histology study demonstrated that the Qufeng Zhitong capsule treatment could reduce the synovium hyperplasia and inflammatory cell infiltration,the inhibitory effects were in accordance with the doses of the drug. The serum levels of pro-inflammatory cytokine of TNF-α in the arthritis rat were not changed significantly,while the serum levels of anti-inflammatory cytokine of IL-10 were elevated significantly after treated with Qufeng Zhitong capsule.
Conclusion: Qufeng Zhitong capsule can inhibit the synovium hyperplasia and cartilage erosion by stimulating the serum levels of anti-inflammatory cytokine of IL-10 and suggest it can be a candidate of treatment of rheumatoid arthritis. |
| KEYWORDS:Antirheumatic agents Arthritis,rheumatoid Interleukin-10 Tumor necrosis factor-alpha Animal experimentation |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 张薇,李涛,李萍,王梅,高小利.祛风止痛胶囊对关节炎模型大鼠抗炎作用及机制的实验研究[J].中国骨伤,2009,22(11):848~851 |
| 英文格式: | ZHANG Wei,LI Tao,LI Ping,WANG Mei,GAO Xiao-li.Anti-inflammatory effect of Qufeng Zhitong(祛风止痛)capsule on the collagen-induced arthritis rat[J].zhongguo gu shang / China J Orthop Trauma ,2009,22(11):848~851 |
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