| 大剂量甲基强的松龙对大鼠急性脊髓损伤后神经细胞Bcl-2表达及细胞凋亡的影响 |
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Received:April 15, 2009
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| 期刊信息：《中国骨伤》2009年22卷，第9期，第692-694页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目:国家自然科学基金资助项目(编号：30500509),河北省自然科学基金资助项目(编号：C2006000840) |
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目的：观察大剂量甲基强的松龙(MP)治疗对大鼠急性脊髓损伤(ASCI)后神经细胞凋亡及凋亡基因Bcl-2的影响。
方法：选取48只雌性SD大鼠随机等分为2组，对照组与治疗组，按Nystr?觟m法制备大鼠急性脊髓损伤模型。治疗组伤后30 min 经腹膜腔注入MP 30 mg/kg,以后每小时腹膜腔注入MP 5.4 mg/kg,维持24 h;对照组应用生理盐水替代MP,处理方法同治疗组。两组分别于伤后4、8 h及1、3、7、14 d灌注固定后取材。免疫组织化学检测损伤段脊髓内Bcl-2蛋白表达，TUNEL检测细胞凋亡，染色结果应用图像分析仪进行半定量分析。
结果：大鼠ASCI后4 h即可见脊髓内TUNEL阳性细胞，8 h表达达高峰，此后表达量逐渐下降，14 d时仍可见少量阳性细胞。凋亡相关蛋白Bcl-2在伤后4 h即可见表达，伤后1 d达高峰，伤后14 d仍有表达。与对照组相比，治疗组伤后8 h、1 d和3 d时凋亡细胞数减少有统计学意义，伤后8 h和1 d Bcl-2蛋白表达增高有统计学意义。
结论：大剂量甲基强的松龙治疗可抑制大鼠ASCI后神经细胞凋亡，并增加凋亡相关蛋白Bcl-2的表达。 |
| [关键词]:脊髓损伤 激素类 细胞凋亡 基因，Bcl-2 动物实验 |
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| Effects of high dose methylprednisolone on cell apoptosis and Bcl-2 expression after acute spinal cord injuries in rats |
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Abstract:
Objective: To investigate the effect of high dose methylprednisolone(MP) on cell apoptosis and Bcl-2 expression after acute spinal cord injury(ASCI).
Methods: The 48 female rats were randomly divided into two groups:control group and experimental group. The spinal cord of rats were wounded at the level of T9,10 in moderate degree for each group. Thirty minutes after ASCI,the subjects in experimental group received MP injection through intraperitoneal with dosage of 30 mg/kg. Then a dosage of 5.4 mg/kg of MP was given through intraperitoneal injection every hour in 24 hours. The subjects in the control group received Normal Saline instead of MP with the same method. The impaired spinal cords were harvested on 4 h,8 h,1 d,3 d,7 d and 14 d after injury,and at each time point 4 rats were sacrificed in each group. The terminal deoxynucleotidal transferase-mediated DUTP-biotin nick end labeling(TUNEL) and immunohistochemical measurement were used to observe neural apoptosis and Bcl-2 expression.
Results: Apoptosis cells were noted primarily at 4 h after injury,with a maximum presence in lesion center at 8 h. The apoptotic index of MP-treated group had distinctly less than that of control group in 8 h,1 d and 3 d after trauma. The Bcl-2 increased in the experimental group.
Conclusion: The administration of the high dose methylprednisolone(MP) can inhibite the apoptosis after acute spinal cord injury in rats and increase the expression of Bcl-2. |
| KEYWORDS:Spinal cord injuries Hormones Apoptosis Genes,Bcl-2 Animal experimentation |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 马利杰,张军军,吴昊天.大剂量甲基强的松龙对大鼠急性脊髓损伤后神经细胞Bcl-2表达及细胞凋亡的影响[J].中国骨伤,2009,22(9):692~694 |
| 英文格式: | MA Li-jie,ZHANG Jun-jun,WU Hao-tian.Effects of high dose methylprednisolone on cell apoptosis and Bcl-2 expression after acute spinal cord injuries in rats[J].zhongguo gu shang / China J Orthop Trauma ,2009,22(9):692~694 |
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