| 活血化瘀中药对激素性股骨头缺血坏死模鼠TGF-β1表达的实验研究 |
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Received:December 25, 2008
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| 期刊信息：《中国骨伤》2009年22卷，第8期，第596-598页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目:福建省卫生厅科技项目(编号：WZY0611) |
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目的：观察活血化瘀中药对激素性股骨头缺血性坏死大鼠转化生长因子β1(TGF-β1)表达的影响，进一步探讨其防治激素性股骨头坏死的作用机制。
方法：采用清洁级SD大鼠40只，随机分为空白组4只和模型组36只，模型组每周2次腹腔注射醋酸泼尼松龙24.5 mg/kg,经6周诱导出早期激素性股骨头缺血坏死的模型，空白组每周2次腹腔注射同等剂量的生理盐水。6周后处死空白组4只和模型组4只，进行光、电镜观察，确定造模成功。随后将其余32只模型组大鼠再随机分为治疗组16只和对照组16只，分别灌服桃红四物汤和生理盐水，于灌胃后第6、8周检测血清中TGF-β1的含量；检测股骨头局部TGF-β1mRNA的转录及股骨头局部TGF-β1的表达。
结果：①图像分析仪分析：治疗组股骨头局部TGF-β1较对照组表达明显增强，两组有明显差异(P<0.01).②血清TGF-β1含量检测：治疗组血清中TGF-β1的表达增强，与对照组相比有明显差异(P<0.01).③股骨头局部TGF-β1 mRNA的转录：治疗组在第6周时表达即增强，在第8周时表达又降低，而对照组在第6周时表达减少，在第8周时未检测到TGF-β1 mRNA的表达，两组有明显差异(P<0.05,P<0.01).
结论：活血化瘀中药可促进激素性股骨头缺血坏死模鼠股骨头局部TGF-β1 mRNA的转录水平以及股骨头局部TGF-β1的表达，促进坏死股骨头的修复。 |
| [关键词]:股骨头坏死 泼尼松龙 转化生长因子β 活血祛瘀剂 |
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| Effect on transforming growth factor-β1 of glucocorticoid-induced avascular necrosis of femoral head in rats by treatment of activating blood circulation of Chinese herbal medicine |
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Abstract:
Objective: To establish rat models of Steroid-avascular necrosis of femoral head,observe the effects of activating blood circulation of chinese herbal medicine on genetic expression of transforming growth factor-β1(TGF-β1). To interpret the mechanism of the effect on Steroid-avascular necrosis of femoral head by activating blood circulation,and offer a effective method to clinical.
Methods: Cleaner-40 SD rats,half males and half females,weight(200±20) g,were randomly divided into 2 groups:4 rats were in common group and 36 rats were in medel group. The rats in medel group were administered with 24.5 mg/kg hydroxyprednisone twice a week peritoneal injection for 6 weeks induced to femur head necrosis. The rats in common group were through gluteus injection as control. There were 4 rats were killed in each group after 6 weeks,to be assure that the model were succed. All surplus rats were divided into treatment group and control group:the treatment group were administrated with activating blood circulation of Chinese herbal medicine 12.3 ml/kg per day,the control group were administrated with sodium chloride 12.3 ml/kg per day. Then,after 6 and 8 weeks,killed the animal and detected all indexes.
Results: 1.The expression of TGF-β by immunohistochemistry and image analysis:the expression of femoral head TGF-β increased significantly in treatment group than in control group and two group had significant differences. 2.Serum levels of TGF-β1:in the treatment group serum expression of TGF-β1 increased,compared with the control group had significant difference(P<0.01). 3.The femoral head local TGF-β1 mRNA transcription:treatment group in the first six weeks,expressed that the increase in the first eight weeks,expressed also reduced,and the control group in the first six weeks,expressed a decrease in the first eight weeks,was not detected to TGF-β1 mRNA expression of difference between the two groups was significant (P<0.01). TGF-β1 mRNA in serum and the femoral head with local expression of TGF-β1 in consistency.
Conclusion: Rat abdominal cavity prednisolone acetate injection plus intermittent standing avascular necrosis modeling stability,good repeatability. Taohongsiwu through the promotion of hormone-ischemic necrosis of the femoral head rat model of TGF-β1 mRNA transcription,and promote expression of TGF-β1. |
| KEYWORDS:Femur head necrosis Prednisolone Transforming growth factor beta Blood act stasis remov agents |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 齐振熙,康靖东,李树强.活血化瘀中药对激素性股骨头缺血坏死模鼠TGF-β1表达的实验研究[J].中国骨伤,2009,22(8):596~598 |
| 英文格式: | QI Zhen-Xi,KANG Jing-dong,LI Shu-qiang.Effect on transforming growth factor-β1 of glucocorticoid-induced avascular necrosis of femoral head in rats by treatment of activating blood circulation of Chinese herbal medicine[J].zhongguo gu shang / China J Orthop Trauma ,2009,22(8):596~598 |
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