| 重楼总皂苷对多发骨折-脂多糖两次打击模型大鼠血清细胞因子水平的影响 |
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Received:May 20, 2008
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| 作者 | Author | 单位 | Unit | E-Mail |
| 周满红 |
ZHOU Man-hong |
遵义医学院附属医院急诊科，贵州 遵义 563003 |
Department of Emergency,the Affiliated Hospital of Zunyi Medical College,Zunyi 563003,Guizhou,China |
manhongzhou@sina.com |
| 贺华经 |
HE Hua-jing |
遵义医学院附属医院急诊科，贵州 遵义 563003 |
Department of Emergency,the Affiliated Hospital of Zunyi Medical College,Zunyi 563003,Guizhou,China |
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| 潘勇 |
PAN Yong |
遵义医学院附属医院急诊科，贵州 遵义 563003 |
Department of Emergency,the Affiliated Hospital of Zunyi Medical College,Zunyi 563003,Guizhou,China |
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| 单立婧 |
SHAN Li-jing |
遵义医学院附属医院急诊科，贵州 遵义 563003 |
Department of Emergency,the Affiliated Hospital of Zunyi Medical College,Zunyi 563003,Guizhou,China |
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| 马懿 |
MA Yi |
遵义医学院附属医院急诊科，贵州 遵义 563003 |
Department of Emergency,the Affiliated Hospital of Zunyi Medical College,Zunyi 563003,Guizhou,China |
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| 刘洋 |
LIU Yang |
遵义医学院附属医院急诊科，贵州 遵义 563003 |
Department of Emergency,the Affiliated Hospital of Zunyi Medical College,Zunyi 563003,Guizhou,China |
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| 期刊信息：《中国骨伤》2008年21卷，第9期，第662-664页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目:贵州省科学技术基金资助(编号：黔科合J[2005]2075) |
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目的：探讨重楼总皂苷(RPTS)对多发骨折-脂多糖两次打击模型大鼠血清TNF-α、IL-1β及IL-6水平的影响。
方法：68只Wistar大鼠随机分成5组，除空白对照组外，其余各组按多发骨折-脂多糖两次打击模型标准制模。制模成功后1 h, 除空白对照组和模型对照组外各组予以不同浓度的RPTS灌胃，干预后6 h采血，以ELISA法检测血清中TNF-α、IL-1β及IL-6浓度。
结果：大鼠血清TNF-α、IL-1β及IL-6浓度，模型对照组与空白对照组相比较明显升高 (P<0.001),而RPTS各干预组与模型对照组相比较却明显下降(P<0.001), 差异均有统计学意义。
结论：重楼总皂苷可以降低多发骨折-脂多糖两次打击模型大鼠血清中的TNF-α、IL-1β及IL-6水平。 |
| [关键词]:重楼总皂苷 骨折 肿瘤坏死因子-α 白细胞介素-1β 白细胞介素-6 |
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| Effects of rhizoma paridis total saponins on levels of cytokines in blood serum of two-hit rat model induced by multiple fractures and lipopolysaccharide |
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Abstract:
Objective: To investigate the effect of rhizoma paridis total saponins(RPTS)on the levels of tumor necrosis factor alpha(TNF-α),interleukin-1 beta(IL-1β) and interleukin-6(IL-6) in blood serum of two-hit rat model induced by multiple fractures and lipopolysaccharide.
Methods: Sixty-eight Wistar rats were randomly divided into five groups. The models were made in four groups(expect the blank control group)in accordance with the standard of two-hit animal model induced by multiple fractures and lipopolysaccharide. At 1 hour after models made,the rats in RPTS groups were given rhizoma paridis total saponins with different concentrations by intragastric administration. Six hours later,the concentrations of TNF-α、IL-1β and IL-6 in the blood serum of all rats were detected by ELISA(enzyme linked immunosorbent assay).
Results: The concentrations of TNF-α、IL-1β and IL-6 in blood serum of rats in the model group were remarkably higher than those in the blank control group(P<0.001),and these in the RPTS groups were remarkably lower than those in the model group(P<0.001).
Conclusion: RPTS can decrease the levels of TNF-α and IL-6 and IL-1β in the blood serum of rats subjected to two-hit induced by multiple fractures and lipopolysaccharide. |
| KEYWORDS:Rhizoma paridis total saponins Fractures Tumor necrosis factor-alpha Interleukin-1 beta Interleukin-6 |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 周满红,贺华经,潘勇,单立婧,马懿,刘洋.重楼总皂苷对多发骨折-脂多糖两次打击模型大鼠血清细胞因子水平的影响[J].中国骨伤,2008,21(9):662~664 |
| 英文格式: | ZHOU Man-hong,HE Hua-jing,PAN Yong,SHAN Li-jing,MA Yi,LIU Yang.Effects of rhizoma paridis total saponins on levels of cytokines in blood serum of two-hit rat model induced by multiple fractures and lipopolysaccharide[J].zhongguo gu shang / China J Orthop Trauma ,2008,21(9):662~664 |
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