| 鹿茸多肽抗鼠软骨细胞老化的机制初探 |
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Received:January 28, 2008
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| 期刊信息：《中国骨伤》2008年21卷，第8期，第617-620页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目:福建省科技计划重大资助项目(编号：2004Y018) |
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目的：对体外传代培养的大鼠软骨细胞进行鹿茸多肽干预对照实验，检测老化相关因子，初步探讨鹿茸多肽抗软骨细胞复制性老化的机制。
方法：进行大鼠软骨细胞取材及传代培养，对第4代软骨细胞进行鹿茸多肽干预，并与第2、3、4代软骨细胞进行对照实验，采用免疫细胞化检测p16、pRb、E2F、CyclinD、CDK4等因子表达水平，TRAP-ELISA(telomerase repeat amplification protocol assay-enzyme linked immunosorbent assay)检测端粒酶活性，进而考察鹿茸多肽对软骨细胞老化过程中各因子的影响。
结果：随着细胞复制性老化p16、pRb、CyclinD表达显着上升(P<0.01),E2F、CDK4、端粒酶表达显着下降(P<0.01);鹿茸多肽干预后p16、pRb、CyclinD表达比第4代软骨细胞表达显着下降(P<0.01),E2F、CDK4、端粒酶表达显着上升(P<0.01).
结论：鹿茸多肽可以逆向影响老化相关调控因子的表达来实现其抗软骨细胞退变老化的作用。 |
| [关键词]:鹿茸多肽 软骨细胞 细胞衰老 |
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| The initial mechanism's investigation of pilose antler polypeptides resisting replicative senescence of rat chondrocyte |
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Abstract:
Objective: To investigate the mechanism of pilose antler polypeptides(PAP) resisting replicative senescence of rat chondrocyte serially subcultivated in vitro by means of PAP interfering and controlled experiment.
Methods: The successive tert-generation(2nd passage,3rd passage,4th passage) chondrocytes and the 4th passage cells intervented by PAP were studied for senenscence mechanism. In this course,immunocytochemistry was applied for p16,pRb,E2F,CyclinD,CDK4 and TRAP-ELISA(telomerase repeat amplification protocol assay- enzyme linked immunosorbent assay) was applied for telomerase activation to observe targets' changing regarding to senescence and the function of PAP.
Results: Along with cell's replicative senescence,p16,pRb and Cyclin D express significantly rised(P<0.01),while E2F,CDK4 and telomerase express significantly lowerd(P<0.01). Meanwhile, in PAP interfered group compared with which in 4th passage group,p16,pRb and Cyclin D express significantly lowerd(P<0.01),while E2F,CDK4 and telomerase express significantly rised(P<0.01).
Conclusion: PAP has function that it reversingly affect the express of factors which controlling cell life cycle and cell growth to postpone chondrocyte senenscence. |
| KEYWORDS:Pilose antler polypeptides Chondrocytes Cell aging |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 陈晓东,林建华.鹿茸多肽抗鼠软骨细胞老化的机制初探[J].中国骨伤,2008,21(8):617~620 |
| 英文格式: | CHEN Xiao-dong,LIN Jian-hua.The initial mechanism's investigation of pilose antler polypeptides resisting replicative senescence of rat chondrocyte[J].zhongguo gu shang / China J Orthop Trauma ,2008,21(8):617~620 |
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