| 联合注射外源性IFNγ和IGF-1治疗小鼠骨骼肌损伤 |
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Received:April 18, 2008
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| 作者 | Author | 单位 | Unit | E-Mail |
| 陈疾忤 |
CHEN Ji-wu |
复旦大学运动医学中心 华山医院运动医学与关节镜外科，上海 200040 |
The Fudan University Sports Medicine Center,Department of Sports Medcine & Arthroscopic Surgery,Huashan Hospital Affiliated to Fudan Univeristy,Shanghai 200040,China |
jeevechan@yahoo.com.cn |
| 陈世益 |
CHEN Shi-yi |
复旦大学运动医学中心 华山医院运动医学与关节镜外科，上海 200040 |
The Fudan University Sports Medicine Center,Department of Sports Medcine & Arthroscopic Surgery,Huashan Hospital Affiliated to Fudan Univeristy,Shanghai 200040,China |
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| 李宏云 |
LI Hong-yun |
复旦大学运动医学中心 华山医院运动医学与关节镜外科，上海 200040 |
The Fudan University Sports Medicine Center,Department of Sports Medcine & Arthroscopic Surgery,Huashan Hospital Affiliated to Fudan Univeristy,Shanghai 200040,China |
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| 尚西亮 |
SHANG Xi-liang |
复旦大学运动医学中心 华山医院运动医学与关节镜外科，上海 200040 |
The Fudan University Sports Medicine Center,Department of Sports Medcine & Arthroscopic Surgery,Huashan Hospital Affiliated to Fudan Univeristy,Shanghai 200040,China |
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| 吴子英 |
WU Zi-ying |
复旦大学运动医学中心 华山医院运动医学与关节镜外科，上海 200040 |
The Fudan University Sports Medicine Center,Department of Sports Medcine & Arthroscopic Surgery,Huashan Hospital Affiliated to Fudan Univeristy,Shanghai 200040,China |
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| 期刊信息：《中国骨伤》2008年21卷，第6期，第434-437页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目:上海市体育局十一五科技攻关计划资助(编号：06JT0170) |
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目的：观察局部联合注射外源性人胰岛素样生长因子-1(human insulin-like growth factor-1,hIGF-1)和人γ干扰素(human interferon γ，hIFNγ)对于小鼠急性钝挫伤骨骼肌修复过程中再生和纤维化的影响。
方法：64只8 周龄雄性C3H小鼠，制作右侧腓肠肌钝挫伤动物模型，随机分为4组，即A组(注射hIFNγ)、B组(注射hIGF-1)、C组(联合注射hIFNγ和hIGF-1)、D组(注射生理盐水).挫伤后第10天，A、B、C、D组小鼠，腓肠肌损伤处分别注射不同药物进行干预。于干预前(伤后7 d),和干预后4、18、32 d,各组随机抽取4只小鼠进行损伤腓肠肌取材，以荧光定量PCR和免疫荧光化学染色方法检测不同时点Ⅱb 型肌球蛋白重链(myosin heavy chain-Ⅱb,MHC-Ⅱb) 及波形蛋白(Vimentin)的表达。
结果：①干预后各时点，B、C组小鼠损伤骨骼肌局部MHC-Ⅱb表达较A、D组明显高；②干预后各时间点A、B、C组小鼠损伤骨骼肌局部Vimentin表达较D组低，其中以A组和B组更明显。
结论：①骨骼肌急性损伤后，局部注射hIGF-1有明显促进骨骼肌纤维再生，和一定程度抑制纤维化的作用。②局部注射hIFNγ仅表现出抑制纤维化的作用，且比hIGF-1更加明显。③联合应用hIGF-1和hIFNγ，能够同时促进骨骼肌再生和抑制纤维化，有效地促进损伤骨骼肌的愈合。 |
| [关键词]:人γ干扰素 胰岛素样生长因子-1 创伤和损伤 肌，骨骼 肌球蛋白重链 波形蛋白 |
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| Effect of exogenous interferon γ on the healing of injured skeletal muscle following injury |
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Abstract:
Objective: To observe the influence of combined injection with human interferon(hIFNγ) and human insulin-link growth factor-1(hIGF-1) on regeneration and fibrosis of skeletal muscle after acute contusion.
Methods: A standard contusion model was reproduced at the right gastrocnemius in 64 male mice of 7 to 12 weeks. All the mice were randomly divided into 4 groups,such as group A(injection with hIFNγ),group B(injection with hIGF-1),group C(injection with hIGF-1 and hIFNγ),and group D(injection with physiological saline as control). All injections were introduced on day 10 after injury at local injured gastrocnemius. Before intervention(7 d following injury),and 4 d,18 d,32 d after intervention,the local injured gastrocnemius were harvested from 4 mice of each group. Then the expression of MHC-Ⅱb and vimentin was detected by real time PCR and immunohistochemistry techniques.
Results: ①At the each time following intervention,the expression of MHC-Ⅱb mRNA and protein in local injured muscle of group B and C were significantly higher than those of group A and D. ② After intervention,the expression of vimentin mRNA and protein in local injured muscle of group A,group B,and group C were more inhibited than those of group D. The inhibition of vimentin expression in group A and C was significant.
Conclusion: ①It is indicated that injection of hIGF-1 into the injured skeletal muscle following acute contusion could enhance muscle regeneration,and inhibit fibrosis to some extent. ② It is identified that hIFNγ injected into injured muscle has the effect of anti-fibrosis,which is more significant than that of hIGF-1. ③Combined injection with hIGF-1 and hIFNγ could improve muscle regeneration and inhibit fibrosis simultaneously,and promote the healing of injured muscle. |
| KEYWORDS:Human interferon γ Human insulin-like growth factor-1 Wounds and injuries Muscle,skeletal Myosin heavy chain Vimentin |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 陈疾忤,陈世益,李宏云,尚西亮,吴子英.联合注射外源性IFNγ和IGF-1治疗小鼠骨骼肌损伤[J].中国骨伤,2008,21(6):434~437 |
| 英文格式: | CHEN Ji-wu,CHEN Shi-yi,LI Hong-yun,SHANG Xi-liang,WU Zi-ying.Effect of exogenous interferon γ on the healing of injured skeletal muscle following injury[J].zhongguo gu shang / China J Orthop Trauma ,2008,21(6):434~437 |
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