| 强骨宝方载药血清灭活与否对成骨细胞增殖功能的影响 |
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Received:November 23, 2007
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| 期刊信息：《中国骨伤》2008年21卷，第6期，第429-431页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目:福建省青年科技人才创新项目(编号：2001J062) |
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目的：探讨强骨宝方糖尿病鼠血清灭活与否对成骨细胞增殖的影响。
方法：采用胰蛋白酶-Ⅱ型胶原酶消化法从1～2日龄SD大鼠颅盖骨中分离出成骨细胞，倒置显微镜下观察其形态，碱性磷酸酶(ALPase)染色法、钙化结节染色、Van-Gieson染色鉴定细胞后用灭活和不灭活的不同时效(灌胃3、5 d,末次灌胃1、3 h)、不同浓度(5%、10%、20%)的强骨宝方糖尿病模型鼠血清加入成骨细胞培养体系，作用一定的时间，应用MTT比色法检测载药血清灭活对成骨细胞增殖能力的影响。
结果：强骨宝方灌胃3、5 d,末次灌胃1、3 h的5%、10%、20%糖尿病鼠不灭活血清与灭活组相比差异有统计学意义，以不灭活载药血清对成骨细胞增殖功能的促进作用较强(P<0.01或P<0.05).
结论：强骨宝方糖尿病模型鼠血清灭活与否影响成骨细胞的增殖功能，以不灭活载药血清的作用最佳。 |
| [关键词]:成骨细胞 细胞增殖 血清学试验 中药疗法 |
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| Experimental study on the effects of inactivated and un-inactivated pharmaco-serum of diabetic rats fed with Chinese herbs Qianggubao decoction(强骨宝方) on the proliferation of osteoblast cultured in vitro |
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Abstract:
Objective: To study the effect of inactivated and un-inactivated pharmaco-serum of diabetic rats fed with Chinese herbs Qianggubao decoction(强骨宝方)on the proliferation of osteoblast cells(OB)cultured in vitro.
Methods: OB was isolated from the skull of newly born SD rats aged 1 to 2 days by means of Trypsin-collagenase digestion and identified by image analysis under inverted microscope,V-G collagen staining,ALP staining,calcification nod staining etc. After the OB was identified,in activated and un-inactivated pharmaco-serum of diabetic rats fed with Qianggubao decoction(强骨宝方) of different phase(rats were fed with medicine 3 days or 5 days after last fed with medicine 1 hour or 3 hours) and concentration(5%,10%,20%) were added to the OB and incubated. After determined times,the effects of the proliferation of osteoblasts were detected by MTT analysis.
Results: There was significant difference between un-inactivated pharmaco-serum and inactivated pharmaco-serum on the proliferation of osteoblasts,and un-inactivated serum had stronger effects to improve the proliferation of osteoblasts(P<0.01 or P<0.05).
Conclusion: Un-inactivated and inactivation pharmaco-serum of diabetic rats fed with Chinese herbs Qianggubao decoction(强骨宝方)can influence the proliferation of,and the un-inactivated pharmaco-serum has stronger effects. |
| KEYWORDS:Osteoblasts Cell proliferation Serologic tests Treatment with Chinese herbs |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 陈智能,苏友新,杨连梓,郑良朴,林久茂,王培清.强骨宝方载药血清灭活与否对成骨细胞增殖功能的影响[J].中国骨伤,2008,21(6):429~431 |
| 英文格式: | CHEN Zhi-neng,SU You-xin,YANG Lian-zi,ZHENG Liang-pu,LIN Jiu-mao,WANG Pei-qing.Experimental study on the effects of inactivated and un-inactivated pharmaco-serum of diabetic rats fed with Chinese herbs Qianggubao decoction(强骨宝方) on the proliferation of osteoblast cultured in vitro[J].zhongguo gu shang / China J Orthop Trauma ,2008,21(6):429~431 |
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