| 四黄散对木瓜蛋白酶诱导的兔急性滑膜炎疗效及作用机制研究 |
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Received:July 16, 2007
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| 作者 | Author | 单位 | Unit | E-Mail |
| 吴权 |
WU Quan |
宁波市中医院，浙江 宁波 315010 |
Ningbo Hospital of TCM,Ningbo 315010,Zhejiang,China |
WuQuan96@sina.com |
| 李启运 |
LI Qi-yun |
宁波市中医院，浙江 宁波 315010 |
Ningbo Hospital of TCM,Ningbo 315010,Zhejiang,China |
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| 周旭光 |
ZHOU Xu-guang |
宁波市中医院，浙江 宁波 315010 |
Ningbo Hospital of TCM,Ningbo 315010,Zhejiang,China |
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| 柯春海 |
HE Chun-hai |
宁波市中医院，浙江 宁波 315010 |
Ningbo Hospital of TCM,Ningbo 315010,Zhejiang,China |
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| 期刊信息：《中国骨伤》2008年21卷，第1期，第42-45页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
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目的：通过用四黄散治疗木瓜蛋白酶诱导的兔急性滑膜炎，有显着疗效的同时，研究四黄散对急性滑膜炎的作用机制。
方法：新西兰白兔32只，分为空白组、造模组、四黄散高剂量组(2 g/kg)、四黄散低剂量组(1 g/kg)、英太青组和野菊花组(溶媒).木瓜蛋白酶注射法制作兔膝关节急性滑膜炎模型，治疗组分别予四黄散(分高低剂量)外敷，野菊花湿敷，英太青软膏外抹。空白组和造模组不予治疗。硝酸还原酶法测NO浓度，ELISA法测IL-1含量，标本病理观察，统计学检验。
结果：四黄散高剂量组使滑膜NO浓度明显降低(P <0.01).四黄散高、低剂量组、英太青组均能降低IL-1含量(P <0.05).滑膜病理观察评分统计，四黄散高剂量组、四黄散低剂量组、野菊花组均能显着降低得分 (P <0.005,P<0.05).四黄散高剂量组显着抑制滑膜内衬炎性细胞聚集，明显改善造模组软骨的退变征象。
结论：四黄散可以降低NO和IL-1浓度，改善滑膜内衬炎性细胞浸润，减轻关节肿胀疼痛，改善关节活动功能，一定程度上减缓软骨的损伤退变。 |
| [关键词]:骨关节炎 外治法 中医现代化 |
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| Study of therapeutic effect and mechanism of Sihuang powder(四黄散)treating acute synovitis in experimental rabbit induced by papain injection |
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Abstract:
Objective: To prove the therapeutic effects of Sihuang powder(四黄散)(composed by four traditional Chine- se herbs:root of baikal skullcap,bark of amur corktree,root of sorrel rhubarb,fruit of cape jasmine,which were mixed with wild Chrysanthemum flower solution)in treating acute synovitis in experimental rabbit knee osteoarthritic models induced by papain injection and to explore its mechanism.
Methods: Thirty-two New-Zealand white rabbits were divided into 6 groups:blank group,model group,Sihuang powder with high dosage group(2 g/kg),Sihuang powder with low dosage group(1 g/kg),Yingtaiqing group and wild Chrysanthemum flower group. The latter four groups were treated respectively with low and high dose Sihuang powder synovium and cartilage were tested concentrations of nitrogen monoxide(NO)and IL-1 level and then were prepared for pathologic and histologic observation 10 days later. Cartilage pathologic changes were record and synovium pathologic changes were valued by means of Mankin's value system.
Results: The NO concentration of synovium in Sihuang powder with high dosage group was lower than that of model group, and there was significantly differences between the two groups(P <0.01). The IL-1 level of synovium was falled after treated with Sihuang powder with high dosage(P<0.05). Sihuang powder with low dosage and Yingtaiqing also could restrain IL-1's release(P<0.05). In Mankin's value system,Sihuang powder with high dosage almost eliminated inflammatory cells infiltrating in synovium,which was seldom found in other groups. The value of Sihuang powder with high dosage group was the lowest in treatment groups(P<0.005). Sihuang powder with low dosage group and wild Chrysanthemum flower group also decreased the degree of inflammatory in synovium(P<0.05).
Conclusion: Sihuang powder can reduce the concentration of NO and IL-1 and improve inflammatory cell infiltrate in lining cells of synovium. Moreover,it can alleviate swelling and pain of joint,improve joint movement and postpone degeneration of the cartilage. |
| KEYWORDS:Osteoarthritis External treatment Modernization of TCM |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 吴权,李启运,周旭光,柯春海.四黄散对木瓜蛋白酶诱导的兔急性滑膜炎疗效及作用机制研究[J].中国骨伤,2008,21(1):42~45 |
| 英文格式: | WU Quan,LI Qi-yun,ZHOU Xu-guang,HE Chun-hai.Study of therapeutic effect and mechanism of Sihuang powder(四黄散)treating acute synovitis in experimental rabbit induced by papain injection[J].zhongguo gu shang / China J Orthop Trauma ,2008,21(1):42~45 |
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