| 股骨头骨骺缺血再灌流关节软骨诱导型一氧化氮合成酶及热休克蛋白70表达的免疫组化研究 |
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Received:January 04, 2007
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| 作者 | Author | 单位 | Unit | E-Mail |
| 张敬东 |
ZHANG Jing-dong |
温州医学院附二院骨科,浙江温州325027 |
Department of Orthopeadic,the Second Hospital of Wenzhou Medical College,Wenzhou 325027,Zhejiang,China |
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| 陈华 |
CHEN Hua |
温州医学院附二院骨科,浙江温州325027 |
Department of Orthopeadic,the Second Hospital of Wenzhou Medical College,Wenzhou 325027,Zhejiang,China |
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| 温宏 |
WEN Hong |
温州医学院附二院骨科,浙江温州325027 |
Department of Orthopeadic,the Second Hospital of Wenzhou Medical College,Wenzhou 325027,Zhejiang,China |
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| 毛方敏 |
MAO Fang-min |
温州医学院附二院骨科,浙江温州325027 |
Department of Orthopeadic,the Second Hospital of Wenzhou Medical College,Wenzhou 325027,Zhejiang,China |
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| 李彬 |
LI Bin |
温州医学院附二院骨科,浙江温州325027 |
Department of Orthopeadic,the Second Hospital of Wenzhou Medical College,Wenzhou 325027,Zhejiang,China |
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| 期刊信息：《中国骨伤》2007年20卷，第8期，第537-539页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目:浙江省教委资助课题(20030807);温州市科技局资助课题(Y2003A158) |
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目的:探讨发育期髋关节股骨头骨骺关节软骨缺血再灌流后的诱导型一氧化氮合成酶(iNOS)及热休克蛋白70(HSP70)的表达。
方法:制作4周龄SD大鼠缺血再灌流及对照模型,每组各40只,于术后3、6、12、24、48h、5d、2、4周不同时点对股骨头骨骺关节软骨iNOS及HSP70进行免疫组织化学检测。
结果:缺血再灌流组24、48h、5d、2周时点的关节软骨浅层、中层iNOS表达增强(P<0.05),缺血再灌流组24、48h、5d有关节软骨浅层、中层HSP70的增强表达(P<0.05)。
结论:发育期髋关节缺血再灌流损害后存在关节软骨的iNOS损害机制及HSP70内源性细胞保护机制。 |
| [关键词]:一氧化氮合成酶 热休克蛋白 缺血再灌流 关节软骨 |
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| Expression of iNOS and HSP70 on articular cartilage of femur head epiphyses after ischemia-reperfusion |
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Abstract:
Objective:To explore the expression of iNOS and HSP70 in articular cartilage of femur head epiphyses after dealed with ischemia-reperfusion.
Methods:Four-weeks Sprague-Dawley rats were classified randomly two groups:experiment group and control group,40 animals in each group.Articular cartilage of femur head epiphyses was collected in 3,6,12,24,48 hours,5 days,2 and 4 weeks after the operation.Expression of iNOS and HSP70 were analyzed by immunohistochemical technique.
Results:The expression of iNOS were significantly increased from 24 hours to 2 weeks after dealed with ischemia-reperfusion in experiment group(P<0.05),the expression of HSP70 were significantly increased from 24 hours to 5 days in experiment group(P<0.05).
Conclusion:Both iNOS injury and HSP70 protection exist in articular cartilage in development stage after ischemia-reperfusion. |
| KEYWORDS:Nitric oxide synthetase,iNOS Heat-shock protein,HSP70 Ischemia-reperfusion Articular cartilage |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 张敬东,陈华,温宏,毛方敏,李彬.股骨头骨骺缺血再灌流关节软骨诱导型一氧化氮合成酶及热休克蛋白70表达的免疫组化研究[J].中国骨伤,2007,20(8):537~539 |
| 英文格式: | ZHANG Jing-dong,CHEN Hua,WEN Hong,MAO Fang-min,LI Bin.Expression of iNOS and HSP70 on articular cartilage of femur head epiphyses after ischemia-reperfusion[J].zhongguo gu shang / China J Orthop Trauma ,2007,20(8):537~539 |
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