| 强骨宝方对体外培养成骨细胞影响的实验研究 |
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Received:July 07, 2006
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| 作者 | Author | 单位 | Unit | E-Mail |
| 苏友新 |
SU You-xin |
福建中医学院骨伤系,福建福州350108 |
Department of Traumatology and Orthopaedics,the Fujan College of Traditional Chinese Medicine,Fuzhou 350108,Fujian,China |
suyouxin777@homtail. com |
| 郑良朴 |
ZHENG Liang-pu |
福建中西医结合研究院 |
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| 陈智能 |
CHEN Zhi-neng |
福建中医学院骨伤系,福建福州350108 |
Department of Traumatology and Orthopaedics,the Fujan College of Traditional Chinese Medicine,Fuzhou 350108,Fujian,China |
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| 杨连梓 |
YANG Lian-zi |
福建中医学院骨伤系 |
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| 王和鸣 |
Wang He-ming |
福建中医学院骨伤系,福建福州350108 |
Department of Traumatology and Orthopaedics,the Fujan College of Traditional Chinese Medicine,Fuzhou 350108,Fujian,China |
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| 期刊信息：《中国骨伤》2007年20卷，第6期，第394-396页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目:福建省青年科技人才创新项目(编号:2001J62) |
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目的:探讨不同浓度强骨宝方提取液直接添加对体外培养成骨细胞增殖、分化与矿化功能的影响。
方法:采用胰蛋白酶-Ⅱ型胶原酶消化法从2只1~2日龄SD乳鼠颅盖骨中分离出成骨细胞,鉴定细胞并传代培养后,分为对照组与4个实验组,实验组分别用终浓度为100、50、10、5μg/ml的强骨宝方提取液加入成骨细胞培养体系,对照组用不含强骨宝方提取液的培养基培养,应用MTT比色法、ALP含量测定、矿化结节形成等分别观察其对成骨细胞增殖、分化及矿化能力的影响。
结果:100、50及10μg/ml浓度的强骨宝方提取液均具有促进体外成骨细胞增殖、分化与矿化的作用,以100μg/ml与50μg/ml促进作用最强。
结论:每毫升含生药2g的强骨宝方提取液,pH=7.0,50μg/ml的添加浓度可能最适合于体外培养成骨细胞的增殖、分化及矿化成骨能力。 |
| [关键词]:成骨细胞 细胞培养 细胞增殖 细胞分化 补肾药 |
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| Effect of Qianggubao decoction(强骨宝方)on osteoblast cultured in vitro |
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Abstract:
Objective: To study the effect of Qianggubao decoction(强骨宝方)extract of different concentration on proliferation,differentiation and mineralization of osteoblast(OB)cultured in vitro.
Methods: The OB was isolated from the skull of two SD rats which were 1 to 2 days after born by means of Trypsin-type Ⅱcollagenase enzymatic digestion.After identified and subcultured,the OB was divided into control group and 4 experimental groups.Different concentration of 100,50,10 and 5 μg/ml Qianggubao decoction(强骨宝方)extrac tw ere added to theOB in 4 exper im enta l g roups. The OB in contro l group w as cultu red w ith no rm al nutr ient m ed ium without Qianggubao decoc tion( 强骨宝方) extract. The e ffects of Qianggubao decoction(强
骨宝方) extract on the pro lifera tion, differentition and m ineralization of osteob lasts w as obse rved byMTT analysis, ALP ana lys is
and m ine ra liza tion forma tion respectively.
Results: TheQ ianggubao decoc tion( 强骨宝方) extract o f 100, 50 and 10g /ml
prom oted proliferation, d ifferen tiation andm inera lization of osteoblast, in which theQianggubao decoc tion( 强骨宝方) extract o f
100g/ml and 50g /ml had the better effects.
Conclusion: The Qianggubao decoction (强骨宝方) ex tract of pH = 70,
50g/mlwh ich conta ins 2 grams crude drug in 1 ml is most suitable for pro liferation, differentition and mineralization of osteo??
blastic cultured in vitro. |
| KEYWORDS:Osteoblasts Cell culture Cell proliferation Cell differentiation Kidney-reinforcing drugs |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 苏友新,郑良朴,陈智能,杨连梓,王和鸣.强骨宝方对体外培养成骨细胞影响的实验研究[J].中国骨伤,2007,20(6):394~396 |
| 英文格式: | SU You-xin,ZHENG Liang-pu,CHEN Zhi-neng,YANG Lian-zi,Wang He-ming.Effect of Qianggubao decoction(强骨宝方)on osteoblast cultured in vitro[J].zhongguo gu shang / China J Orthop Trauma ,2007,20(6):394~396 |
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