| 循环静水压力对维持兔软骨细胞表型的影响 |
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Received:July 20, 2006
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| 作者 | Author | 单位 | Unit | E-Mail |
| 黄正 |
HUANG Zheng |
上海市交通大学医学院附属瑞金医院伤骨科研究所,上海200025 |
The Institute of Orthopaedics and Trauma,the Affiliated Ruijin Hospital of Jiaotong University of Shanghai,Shanghai 200025,China |
hardeesss@126.com |
| 傅文彧 |
FU Wen-yu |
上海市交通大学医学院附属瑞金医院伤骨科研究所,上海200025 |
The Institute of Orthopaedics and Trauma,the Affiliated Ruijin Hospital of Jiaotong University of Shanghai,Shanghai 200025,China |
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| 冯伟 |
FENG Wei |
上海市交通大学医学院附属瑞金医院伤骨科研究所,上海200025 |
The Institute of Orthopaedics and Trauma,the Affiliated Ruijin Hospital of Jiaotong University of Shanghai,Shanghai 200025,China |
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| 张凤华 |
ZHANG Feng-hua |
上海市交通大学医学院附属瑞金医院伤科 |
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| 朱雅萍 |
ZHU Ya-ping |
上海市交通大学医学院附属瑞金医院伤骨科研究所,上海200025 |
The Institute of Orthopaedics and Trauma,the Affiliated Ruijin Hospital of Jiaotong University of Shanghai,Shanghai 200025,China |
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| 魏立 |
WEI Li |
上海市交通大学医学院附属瑞金医院伤骨科研究所,上海200025 |
The Institute of Orthopaedics and Trauma,the Affiliated Ruijin Hospital of Jiaotong University of Shanghai,Shanghai 200025,China |
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| 周琦 |
ZHOU Qi |
上海市交通大学医学院附属瑞金医院伤骨科研究所,上海200025 |
The Institute of Orthopaedics and Trauma,the Affiliated Ruijin Hospital of Jiaotong University of Shanghai,Shanghai 200025,China |
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| 黄好 |
HUANG Hao |
上海市仁和医院口腔科 |
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| 杜宁 |
DU Ning |
上海市交通大学医学院附属瑞金医院伤科 |
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| 期刊信息：《中国骨伤》2007年20卷，第3期，第180-183页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目:国家自然基金资助(编号:30271628);;上海市自然科学基金(编号:03ZR14084);;上海市教委基金(编号:02XJ21021,04BB22);;上海市卫生局课题(编号:034077) |
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目的:本研究将兔软骨细胞放在静水压力负载装置中培养,并传代,施加不同频率和大小的静水压,观察其对兔软骨细胞表型的影响。
方法:用酶消化法获取兔关节软骨细胞,分别在不同的压力和频率下作普通培养瓶贴壁的单层培养,并传代。40 kPa循环静水加压为实验组,40 kPa持续静水加压培养和常压培养作为对照。Ⅰ型胶原和Ⅱ型胶原免疫组化,苏木素套核染色,倒置显微镜观察摄像。以RT-PCR方法检测软骨细胞中蛋白聚糖、聚集蛋白聚糖、Ⅰ型胶原和Ⅱ型胶原mRNA的表达。
结果:在循环静水压力下培养,软骨细胞有较高的细胞增殖率,培养至第7代未见明显表型改变,仍然表达Ⅱ型胶原、蛋白聚糖和聚集蛋白聚糖等。常压培养,软骨细胞传代至第5代以后,逐渐失去软骨细胞的特有表型。而持续静水压力培养的软骨细胞传代至第3代以后就开始逐渐失去软骨细胞的特有表型。
结论:一定的循环静水压力能维持软骨细胞的合成分泌功能,有助于软骨细胞特有表型的稳定。 |
| [关键词]:软骨细胞 压力 细胞培养技术 |
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| Effects of cyclic hydrostatic pressure on the stability of chondrocyte phenotype in vitro |
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Abstract:
Objective: To investigate how to maintain the chondrocytes phenotype under cyclic hydrostatic pressure.
Methods: Isolated rabbit chondrocytes which were obtained by using protease digestion method were cultured in monolayer and propagated under cyclic hydrostatic pressure(in experimental group),sustained hydrostatic pressure,or normal pressure(in control group).The proliferation and the intracellular matrix of chondrocytes were observed under light microscopy.The expression of proteoglycan,aggrecan,collagen type Ⅰ and Ⅱ were detected with reverse transcription-polymerase chain reaction.
Results: Under the cylcic hydrostatic pressure culture,the growth rate was higher than that of other groups without chondrocyte phenotype changing until cultured to the 7th passage.Under the sustained hydrostatic pressure culture,the chondrocytes changed phenotype gradually after cultured to the 3rd passage.Under the normal pressure culture,the chondrocytes changed phenotype after cultured to the 5th passage.
Conclusion: Cyclic hydrostatic pressure system is a promising method for the maintenance of the chondrocytes phenotype. |
| KEYWORDS:Chondrocytes Pressure Cell culture techniques |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 黄正,傅文彧,冯伟,张凤华,朱雅萍,魏立,周琦,黄好,杜宁.循环静水压力对维持兔软骨细胞表型的影响[J].中国骨伤,2007,20(3):180~183 |
| 英文格式: | HUANG Zheng,FU Wen-yu,FENG Wei,ZHANG Feng-hua,ZHU Ya-ping,WEI Li,ZHOU Qi,HUANG Hao,DU Ning.Effects of cyclic hydrostatic pressure on the stability of chondrocyte phenotype in vitro[J].zhongguo gu shang / China J Orthop Trauma ,2007,20(3):180~183 |
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